Interaction of Streptavidin-Based Peptide-MHC Oligomers (Tetramers) with Cell-Surface T Cell Receptors

he binding of oligomeric peptide–MHC (pMHC) complexes to cell surface TCR can be considered to approximate TCR–pMHC interactions at cell-cell interfaces. In this study, we analyzed the equilibrium binding of streptavidin-based pMHC oligomers (tetramers) and their dissociation kinetics from CD8[superscript pos] T cells from 2C-TCR transgenic mice and from T cell hybridomas that expressed the 2C TCR or a high-affinity mutant (m33) of this TCR. Our results show that the tetramers did not come close to saturating cell-surface TCR (binding only 10–30% of cell-surface receptors), as is generally assumed in deriving affinity values (K[subscript D]), in part because of dissociative losses from tetramer-stained cells. Guided by a kinetic model, the oligomer dissociation rate and equilibrium constants were seen to depend not only on monovalent association and dissociation rates (k[subscript off] and k[subscript on]), but also on a multivalent association rate (μ) and TCR cell-surface density. Our results suggest that dissociation rates could account for the recently described surprisingly high frequency of tetramer-negative, functionally competent T cells in some T cell responses.National Institutes of Health (U.S.) (Grant P01 CA097296)National Institutes of Health (U.S.) (Grant R01 GM55767)National Institutes of Health (U.S.) (Grant PO1-AI071195)National Institutes of Health (U.S.). Pioneer Awar

コトバ ノ ハッタツ ト オクレ

Speech is the ability that is given only to humanity. Biped locomotion had changed the form of mouth and throat, which enlarged cerebrum. Language is the means of thinking as well as communication. We learn language to use it not only in our daily life, but also in studying. There are some causes of delay in speech development : hearing disturbance, intellectual deficiency, autism, cerebral palsy, and so on. As there is the critical period, it is important to find the cause and deal with it soon. To promote speech development, both stable everyday life and positive direction are necessary

Circular Distribution of Corona Current of Multiple-Conductor Transmission Line (Ⅵ)

departmental bulletin pape

Increased abundance of frost mRNA during recovery from cold stress is not essential for cold tolerance in adult Drosophila melanogaster.

Frost (Fst) is a candidate gene associated with the response to cold in Drosophila melanogaster because Fst mRNA accumulation increases during recovery from low temperature exposure. We investigated the contribution of Fst expression to chill-coma recovery time, acute cold tolerance and rapid cold hardening (RCH) in adult D. melanogaster by knocking down Fst mRNA expression using GAL4/UAS-mediated RNA interference. In this experiment, four UAS-Fst and one tubulin-GAL4 lines were used. We predicted that if Fst is essential for cold tolerance phenotypes, flies with low Fst mRNA levels should be less cold tolerant than flies with normal levels of cold-induced Fst mRNA. Cold-induced Fst abundance and recovery time from chill-coma were not negatively correlated in male or female flies. Survival of 2 h exposures to sub-zero temperatures in Fst knockdown lines was not lower than that in a control line. Moreover, a low temperature pretreatment increased survival of severe cold exposure in flies regardless of Fst abundance level during recovery from cold stress, suggesting that Fst expression is not essential for RCH. Thus, cold-induced Fst accumulation is not essential for cold tolerance measured as chill-coma recovery time, survival to acute cold stress and RCH response in adult D. melanogaster

セイジン ヘイソクセイ スイミンジ コキュウ ショウガイ ノ ゲンジョウ ニツイテ

There are about 2 million patients with Obstructive sleep apnea hypopnea syndrome (OSAHS) in Japan. The morbidity rate of the OSAHS is considered to increase according to the life style change. After the Bullet-train accident caused by OSAHS patient, the prbrem of sleep apnea hypopnea syndrome has been well recognised. Establishment of diagnostic and treatment method for OSAHS is needed. In this papar, mechanism of upper respiratory obstruction, diagnostic method of outclinic patient and treatment strategy are disscussed. Recently, portable polysomnography recording method and nasal contiuous positive airway pressure (nCPAP) method are widely used for diagnosis and treatment respectively. The weak point and countermeasure of the portable recording and nCPAP are disscussed

Analysis of Nucleotides by Anion Exchange Chromatography

departmental bulletin pape

Study on the compressibility and the consolidation characteristics of clays with developed structure due to cementation,and with prominent creep behavior

広島大学(Hiroshima University)博士(工学)Engineeringdoctora