Writing Victimhood: A methodological manifesto for researching digital sexual assault

Non-consensual sharing of intimate images, here called digital sexual assault (DSA), has been a heavily debated subject in Denmark over the past few years. In the polarized public and academic debate, DSA victims are often either subjected to victim blaming or portrayed as ‘broken’ victims with little agency and hope of redemption. This article presents a methodology for working with DSA victims to construct their own alternative and empowering stories of victimhood.Through an experimental methodology based on creative writing, I have included three young women in a process of collectively developing and exploring aspects of their experiences with DSA. With this methodology, I aim to combine activism and research in investigating DSA and actively supporting victims in constructing progressive stories of victimhood; stories that, as activism, work in opposition to oppressive discourses, and, as research, offer insights into complex experiences of victimhood. The article ends in a discussion of change as the prospect of activist research and experimental methodologies and concludes with a “manifesto for writing victimhood” stating activist aims that encourage and value social, personal and political change in and through research

Targeted, homology-driven gene insertion in stem cells by ZFN-loaded 'all-in-one' lentiviral vectors

Biased integration remains a key challenge for gene therapy based on lentiviral vector technologies. Engineering of next-generation lentiviral vectors targeting safe genomic harbors for insertion is therefore of high relevance. In a previous paper (Cai et et, 2014a), we showed the use of integrase-defective lentiviral vectors (IDLVs) as carriers of complete gene repair kits consisting of zinc-finger nuclease (ZFN) proteins and repair sequences, allowing gene correction by homologous recombination (HR). Here, we follow this strategy to engineer ZEN-loaded IDLVs that insert transgenes by a homology-driven mechanism into safe loci. This insertion mechanism is driven by time-restricted exposure of treated cells to ZFNs. We show targeted gene integration in human stem cells, including CD34+ hematopoietic progenitors and induced pluripotent stem cells (iPSCs). Notably, targeted insertions are identified in 89% of transduced iPSCs. Our findings demonstrate the applicability of nuclease-loaded 'all-in-one' IDLVs for site-directed gene insertion in stem cell based gene therapies

11.Hypoxic ventilatory depressionと思われる一症例について(第551回千葉医学会例会・第9回麻酔科例会・第18回千葉麻酔懇話会)

FISH analysis on metaphase nuclei (top panel) of cultured cells derived from peripheral blood leukocytes of the proband of family 2 by using BAC probes for 11p15.5-15.4 (RP11-11A9, 3,236,552-3,356,012, green) and 11q22.3 (RP11-179B7, 104,298,339-104,459,797, red). The green signal on both homologues is visible only at chr11p, demonstrating the presence of an in cis duplication and excluding an unbalanced translocation. FISH analysis on interphase nuclei (bottom panel) using the BACs RP11-699D10 (2.9–3.0 Mb, red) and RP11-11A9 (green), hybridizing within the duplication. Note that single and duplicated signals can be seen on the two homologues, respectively. The red-green-green-red order of the duplicated signals indicates that the duplication is inverted. (PDF 52 kb

Editorial Foreword: Challenging Academic Participation

This issue contributes to the growing criticisms of and challenges to participatory methods and cultural participation by focusing on ‘academic participation’. By academic participation, we refer to the use of participatory methodologies in academic research, but we also aim to expand the term by including reflections on the modes and conditions of taking part — willingly and unwillingly — in academic systems and institutions as such. The articles of this issue invite the reader to reconsider what and how participation looks like in the academy. Taken together, they suggest that we might need to broaden how we understand, apply and critique participation in academic research: from the participatory methods applied in specific research projects, to how we might foster a more participatory academic system that rejects the current individualization, financialization, and exploitation at play

Urine dipstick for predicting intrapartum recto-vaginal colonisation by group B streptococci

Introduction: In pregnant women, bacteriuria with group B streptococci (GBS) may be associated with a high degree of recto-vaginal GBS colonisation and therefore an increased risk of early-onset GBS disease. The aim of this study was to assess the performance of routine use of dipstick urine analysis during pregnancy for prediction of recto-vaginal GBS colonisation at the time of labour. Methods: Among 902 unselected Danish pregnant women, we obtained results from 1) dipstick urine analysis, 2) urine culture carried out during pregnancy, if indicated, and 3) recto-vaginal culture at labour. The inclusion criteria were age > 18 years and gestational age ≥ 37 weeks. Results: Intrapartum recto-vaginal GBS colonisation was predicted by a positive urine dipstick with 5% sensitivity only. Conclusion: Dipstick urine analysis had a low sensitivity for predicting intrapartum recto-vaginal colonisation with GBS. FUNDING: none. TRIAL REGISTRATION: not relevant