Single-kernel ionomic profiles are highly heritable indicators of genetic and environmental influences on elemental accumulation in maize grain (Zea mays)

Peer reviewedPublisher PD

Nuclear localised more sulphur accumulation1 epigenetically regulates sulphur homeostasis in Arabidopsis thaliana

Sulphur (S) is an essential element for all living organisms. The uptake, assimilation and metabolism of S in plants are well studied. However, the regulation of S homeostasis remains largely unknown. Here, we report on the identification and characterisation of the more sulphur accumulation1 (msa1-1) mutant. The MSA1 protein is localized to the nucleus and is required for both S adenosylmethionine (SAM) production and DNA methylation. Loss of function of the nuclear localised MSA1 leads to a reduction in SAM in roots and a strong S-deficiency response even at ample S supply, causing an over- accumulation of sulphate, sulphite, cysteine and glutathione. Supplementation with SAM suppresses this high S phenotype. Furthermore, mutation of MSA1 affects genome-wide DNA methylation, including the methylation of S-deficiency responsive genes. Elevated S accumulation in msa1-1 requires the increased expression of the sulphate transporter genes SULTR1;1 and SULTR1;2 which are also differentially methylated in msa1-1. Our results suggest a novel function for MSA1 in the nucleus in regulating SAM biosynthesis and maintaining S homeostasis epigenetically via DNA methylation

Research On Recycling Of Hardened Mortar From Construction And Demolition Waste

Abstract The recycling issues related to the construction and demolition (C&amp;D) wastes in the sustainable development and the circular economy context represent a continuous challenge for researchers. This paper reveals the possibility to recycle the hardened mortar recovered from C&amp;D wastes. Thus, the recovered hardened mortar with grains size less than 16 mm was used in the concrete structure. The compression resistance of the final concrete was determined using a Heckert 3000 KN testing machine and the results showed a better compressive strength for the samples with C&amp;D waste content than the standard sample of about 1.19 times. This is a good premise for improving the researches related to C&amp;D waste usage in concrete production.</jats:p