3,836 research outputs found

    Subsonic near-surface P-velocity and low S-velocity observations using propagator inversion

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    Detailed knowledge of near-surface P- and S-wave velocities is important for processing and interpreting multicomponent land seismic data because (1) the entire wavefield passes through and is influenced by the near-surface soil conditions, (2) both source repeatability and receiver coupling also depend on these conditions, and (3) near-surface P- and S-wave velocities are required for wavefield decomposition and demultiple methods. However, it is often difficult to measure these velocities with conventional techniques because sensitivity to shallow-wave velocities is low and because of the presence of sharp velocity contrasts or gradients close to the earth's free surface. We demonstrate that these near-surface P- and S-wave velocities can be obtained using a propagator inversion. This approach requires data recorded by at least one multicomponent geophone at the surface and an additional multicomponent geophone at depth. The propagator between them then contains all information on the medium parameters governing wave propagation between the geophones at the surface and at depth. Hence, inverting the propagator gives local estimates for these parameters. This technique has been applied to data acquired in Zeist, the Netherlands. The near-surface sediments at this site are unconsolidated sands with a thin vegetation soil on top, and the sediments considered are located above the groundwater table. A buried geophone was positioned 1.05 m beneath receivers on the surface. Propagator inversion yielded low near-surface velocities, namely, 270 ± 15 m/s for the compressional-wave velocity, which is well below the sound velocity in air, and 150 ± 9 m/s for the shear velocity. Existing methods designed for imaging deeper structures cannot resolve these shallow material properties. Furthermore, velocities usually increase rapidly with depth close to the earth's surface because of increasing confining pressure. We suspect that for this reason, subsonic near-surface P-wave velocities are not commonly observed

    The predictive value of pain event-related potentials for the clinical experience of pain

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    Event-related potentials (ERPs) have been found to be related to subjective experience of experimental pain. But how are they related to the subjective experience of clinical pain? The current study investigated the predictive value of the pain ERP for the subjective experience of clinical pain. Event-related potentials in response to experimental pain were measured in 75 chronic low back pain sufferers. In addition, a two-week registration to note the amount of pain they experienced in daily life was done. The results demonstrate that the N2-component at Cz and C4 of the pain ERP (contralateral to the side of the stimulation) were significant predictors of clinical pain, and even stronger predictors than the accompanying subjective ratings of experimental pain. Thus, it seems promising to use event-related potentials as a more objective measure to make predictions about a person's likely pain experience in daily life

    Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes

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    New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistanc

    Allele mining in solanum: conserved homologues of Rpi-blb 1 are identified in Solanum stoloniferum

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    Allele mining facilitates the discovery of novel resistance (R) genes that can be used in breeding programs and sheds light on the evolution of R genes. Here we focus on two R genes, Rpi-blb1 and Rpi-blb2, originally derived from Solanum bulbocastanum. The Rpi-blb1 gene is part of a cluster of four paralogues and is flanked by RGA1-blb and RGA3-blb. Highly conserved RGA1-blb homologues were discovered in all the tested tuber-bearing (TB) and non-tuber-bearing (NTB) Solanum species, suggesting RGA1-blb was present before the divergence of TB and NTB Solanum species. The frequency of the RGA3-blb gene was much lower. Interestingly, highly conserved Rpi-blb1 homologues were discovered not only in S. bulbocastanum but also in Solanum stoloniferum that is part of the series Longipedicellata. Resistance assays and genetic analyses in several F1 populations derived from the relevant late blight resistant parental genotypes harbouring the conserved Rpi-blb1 homologues, indicated the presence of four dominant R genes, designated as Rpi-sto1, Rpi-plt1, Rpi-pta1 and Rpi-pta2. Furthermore, Rpi-sto1 and Rpi-plt1 resided at the same position on chromosome VIII as Rpi-blb1 in S. bulbocastanum. Segregation data also indicated that an additional unknown late blight resistance gene was present in three populations. In contrast to Rpi-blb1, no homologues of Rpi-blb2 were detected in any material examined. Hypotheses are proposed to explain the presence of conserved Rpi-blb1 homologues in S. stoloniferum. The discovery of conserved homologues of Rpi-blb1 in EBN 2 tetraploid species offers the possibility to more easily transfer the late blight resistance genes to potato varieties by classical breeding

    Integration and Usage of an Industrial Network Management System in an Accelerator Controls Environment

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    In the last years the CERN accelerator networks infrastructure has been upgraded to contemporary industrial standards. A commercially available network management tool has been selected to monitor and optimise the usage of the infrastructure. HPOpenview Network Node Manager (NNM) provides concise and indepth views of network and devices connected with their operational status. It provides instant failure detection, can supply alarm information and gathers statistics to allow proactive maintenance thus reducing network congestion and downtime. The heterogeneous community of equipment as installed around CERN's PS, SPS and LEP accelerator complex can be monitored in a uniform manner from a single entry point. The integration of a network specialist tool into the accelerator operations environment required additional developments in information reduction and presentation to create intuitive graphical displays related to the accelerators geographical and functional situation. This report describes the integration of the most recent version of the HPOpenview NNM in the CERN Accelerator's Controls System and details the accelerator controls specific developments

    Finding Stories in 1,784,532 Events: Scaling Up Computational Models of Narrative

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    Information professionals face the challenge of making sense of an ever increasing amount of information. Storylines can provide a useful way to present relevant information because they reveal explanatory relations between events. In this position paper, we present and discuss the four main challenges that make it difficult to get to these stories and our first ideas on how to start resolving them

    Surface-consistent amplitude corrections for single or multicomponent sources and receivers using reciprocity and waveform inversion

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    In land seismics, near-surface conditions often vary within surveys, resulting in differences in source strength and signature. Furthermore, discrepancies between closely spaced recordings are also commonly observed. Processing and interpretation of recorded data require that data are corrected for these source and receiver perturbations in the early stages of processing. However, existing surface-consistent deconvolution techniques are applicable to primary reflection data only, and therefore require that ground roll and multiples are suppressed prior to the application. This is usually performed with multichannel filter operations. The performance of these filter operations, however, rapidly deteriorates in presence of acquisition-related amplitude and phase perturbations. We propose an alternative approach to compensate for acquisition-related amplitude perturbations, which has the advantage of being purely a pre-processing step. It has the following characteristics: (i) it can be applied to complete recordings, hence does not require the isolation of primary reflections in the data, (ii) no assumptions are imposed on the subsurface and (iii) it is applicable to multicomponent data. The procedure is based on reciprocity of the medium response, so that differences between normal and reciprocal traces can be attributed to source and receiver perturbations. The application of reciprocity requires symmetric data acquisition, that is, identical source and receiver patterns, identical locations, and the source orientations have to be identical to the receiver components. Besides reciprocity, additional constraints are required to determine the lateral source and receiver amplitude variations fully. We use criteria based on minimizing total energy differences between adjacent common source and common receiver gathers, and in common offset panels of the medium response. Synthetic tests demonstrate that acquisition-related amplitude differences can be significantly reduced using this method

    Gene transcription analysis during interaction between potato and Ralstonia solanacearum

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    Bacterial wilt (BW) caused by Ralstonia solanacearum (Rs) is an important quarantine disease that spreads worldwide and infects hundreds of plant species. The BW defense response of potato is a complicated continuous process, which involves transcription of a battery of genes. The molecular mechanisms of potato-Rs interactions are poorly understood. In this study, we combined suppression subtractive hybridization and macroarray hybridization to identify genes that are differentially expressed during the incompatible interaction between Rs and potato. In total, 302 differentially expressed genes were identified and classified into 12 groups according to their putative biological functions. Of 302 genes, 81 were considered as Rs resistance-related genes based on the homology to genes of known function, and they have putative roles in pathogen recognition, signal transduction, transcription factor functioning, hypersensitive response, systemic acquired resistance, and cell rescue and protection. Additionally, 50 out of 302 genes had no match or low similarity in the NCBI databases, and they may represent novel genes. Of seven interesting genes analyzed via RNA gel blot and semi-quantitative RT-PCR, six were induced, one was suppressed, and all had different transcription patterns. The results demonstrate that the response of potato against Rs is rapid and involves the induction of numerous various genes. The genes identified in this study add to our knowledge of potato resistance to Rs
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