Internet of Things and Machine Learning Applications for Smart Precision Agriculture

Agriculture forms the major part of our Indian economy. In the current world, agriculture and irrigation are the essential and foremost sectors. It is a mandatory need to apply information and communication technology in our agricultural industries to aid agriculturalists and farmers to improve vice all stages of crop cultivation and post-harvest. It helps to enhance the country’s G.D.P. Agriculture needs to be assisted by modern automation to produce the maximum yield. The recent development in technology has a significant impact on agriculture. The evolutions of Machine Learning (ML) and the Internet of Things (IoT) have supported researchers to implement this automation in agriculture to support farmers. ML allows farmers to improve yield make use of effective land utilisation, the fruitfulness of the soil, level of water, mineral insufficiencies control pest, trim development and horticulture. Application of remote sensors like temperature, humidity, soil moisture, water level sensors and pH value will provide an idea to on active farming, which will show accuracy as well as practical agriculture to deal with challenges in the field. This advancement could empower agricultural management systems to handle farm data in an orchestrated manner and increase the agribusiness by formulating effective strategies. This paper highlights contribute to an overview of the modern technologies deployed to agriculture and suggests an outline of the current and potential applications, and discusses the challenges and possible solutions and implementations. Besides, it elucidates the problems, specific potential solutions, and future directions for the agriculture sector using Machine Learning and the Internet of things

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data