Three dimensional tracking of gold nanoparticles using digital holographic microscopy

In this paper we present a digital holographic microscope to track gold colloids in three dimensions. We report observations of 100nm gold particles in motion in water. The expected signal and the chosen method of reconstruction are described. We also discuss about how to implement the numerical calculation to reach real-time 3D tracking

Imaging velocities of a vibrating object by stroboscopic sideband holography

We propose here to combine sideband holography with stroboscopic illumination synchronized with the vibration of an object. By sweeping the optical frequency of the reference beam such a way the holographic detection is tuned on the successive sideband harmonic ranks, we are able to image the instantaneous velocities of the object. Since the stroboscopic illumination is made with an electronic device, the method is compatible with fast (up to several MHz) vibration motions. The method is demonstrated with a vibrating clarinet reed excited sinusoidally at 2 kHz, and a stroboscopic illumination with cyclic ratio 0.15. Harmonic rank up to n = $\pm$100 are detected, and a movie of the instantaneous velocities is reported

Fish embryo multimodal imaging by laser Doppler digital holography

A laser Doppler imaging scheme combined to an upright microscope is proposed. Quantitative Doppler imaging in both velocity norm and direction, as well as amplitude contrast of either zebrafish flesh or vasculature is demonstrated.Comment: Signal Recovery and Synthesis, Jul 2014, Seattle, United States. (Optical Society of America), OSA Technical Digest (online) 2014, paper JTu4A.7., Imaging and Applied Optics 2014, http://www.opticsinfobase.org/abstract.cfm?URI=SRS-2014-JTu4A.

Holographic microscopy for the three-dimensional exploration of light scattering from gold nanomarkers in biological media

The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and the phase-shifting acquisition of the digital holograms. Images are obtained using a novel 3D reconstruction method providing longitudinally undistorted 3D images. A comparative study of the 3D reconstructions of the scattered fields allows us to locate the gold markers which yield, contrarily to the cellular structures, well defined bright scattering patterns that are not angularly titled and clearly located along the optical axis. This characterization is an unambiguous signature of the presence of the gold biological nanomarkers, and validates the capability of digital holographic microscopy to discriminate them from background signals in live cells

Two-step distortion-free reconstruction scheme for holographic microscopy

We propose a three-dimensional holographic reconstruction procedure applicable with no a priori knowledge about the recording conditions enabling distortion-free three-dimensional object reconstruction

Exploring shot noise and Laser Doppler imagery with heterodyne holography

Heterodyne Holography is a variant of Digital Holography, where the optical frequencies of signal and reference arms can be freely adjusted by acousto-optic modulators. Heterodyne Holography is an extremely versatile and reliable holographic technique, which is able the reach the shot noise limit in sensitivity at very low levels of signal. Frequency tuning enables Heterodyne Holography to become a Laser Doppler imaging technique that is able to analyze various kinds of motion.Comment: Information Optics (WIO), 2010 9th Euro-American Workshop on, Helsinki : Finland (2010

Digital Holography at Shot Noise Level

By a proper arrangement of a digital holography setup, that combines off-axis geometry with phase-shifting recording conditions, it is possible to reach the theoretical shot noise limit, in real-time experiments.We studied this limit, and we show that it corresponds to 1 photo-electron per pixel within the whole frame sequence that is used to reconstruct the holographic image. We also show that Monte Carlo noise synthesis onto holograms measured at high illumination levels enables accurate representation of the experimental holograms measured at very weak illumination levels. An experimental validation of these results is done

Video-rate laser Doppler vibrometry by heterodyne holography

We report a demonstration video-rate heterodyne holography in off-axis configuration. Reconstruction and display of 1 Megapixel holograms is achieved at 24 frames per second, with a graphics processing unit. Our claims are validated with real-time screening of steady-state vibration amplitudes in a wide-field, non-contact vibrometry experiment.Comment: Optics Letters (2011) 00