Lung development in laminin γ2 deficiency: abnormal tracheal hemidesmosomes with normal branching morphogenesis and epithelial differentiation

BACKGROUND: Laminin γ2 (Lamc2), one of the polypeptides in laminin-332 (laminin-5), is prominent in the basement membrane of alveolar walls and airways of developing and adult lung. Laminins are important for lung morphogenesis and based on its localization, a function for laminin γ2 in lung development has been hypothesized. Targeted deletion of the laminin γ2 gene in mice results in skin blistering and neonatal death at 3–5 days after birth due to failure to thrive. METHODS: Examination of lung development in Lamc2-/- mice through 1–2 days postnatal was accomplished by morphometric analysis, lung bud culture, electron microscopy, immunohistochemical and immunofluorescence staining. RESULTS: Compared to littermate controls, Lamc2-/- lungs were similar in morphology during embryonic life. At post-natal day 1–2, distal saccules were mildly dilated by chord length measurements. Epithelial differentiation as evaluated by immunohistochemical staining for markers of ciliated cells, Clara cells, alveolar type I cells and alveolar type II cells did not reveal a difference between Lamc2-/- and littermate control lungs. Likewise, vascular development, smooth muscle cell differentiation, and elastic fiber formation looked similar, as did airway basement membrane ultrastructure. Branching morphogenesis by lung bud culture was similar in Lamc2-/- and littermate control lungs. Since laminin-332 is important for hemidesmosome formation, we examined the structure of tracheal hemidesmosomes by transmission electron microscopy. Compared to littermate controls, Lamc2-/- tracheal hemidesmosomes were less organized and lacked the increased electron density associated with the basement membrane abutting the hemidesmosome. CONCLUSION: These findings indicate that laminin γ2 and laminin-332, despite their prominence in the lung, have a minimal role in lung development through the saccular stage

Early peripheral endothelial dysfunction predicts myocardial infarct extension and microvascular obstruction in patients with ST-elevation myocardial infarction.

INTRODUCTION AND OBJECTIVES: The role of endothelial dysfunction (ED) in patients with ST-elevation myocardial infarction (STEMI) is poorly understood. Peripheral arterial tonometry (PAT) allows non-invasive evaluation of ED, but has never been used for this purpose early after primary percutaneous coronary intervention (P-PCI). Our purpose was to analyze the relation between ED assessed by PAT and both the presence of microvascular obstruction (MVO) and infarct extension in STEMI patients. METHODS: ED was assessed by the reactive hyperemia index (RHI), measured by PAT and defined as RHI <1.67. Infarct extension was assessed by troponin I (TnI) release and contrast-enhanced cardiac magnetic resonance (ceCMR). MVO was assessed by ceCMR and by indirect angiographic and ECG indicators. An echocardiogram was also performed in the first 12 h. RESULTS: We included 38 patients (mean age 60.0±13.7 years, 29 male). Mean RHI was 1.87±0.60 and 16 patients (42.1%) had ED. Peak TnI (median 118 mg/dl, IQR 186 vs. 67/81, p=0.024) and AUC of TnI (median 2305, IQR 2486 vs. 1076/1042, p=0.012) were significantly higher in patients with ED, who also showed a trend for more transmural infarcts (63.6% vs. 22.2%, p=0.06) and larger infarct mass on ceCMR (median 17.5%, IQR 15.4 vs. 10.1/10.3, p=0.08). Left ventricular ejection fraction (LVEF) was lower and wall motion score index (WMSI) was higher on both echocardiogram and ceCMR in patients with ED. On ceCMR, MVO was more frequent in patients with RHI <1.67 (54.5% vs. 11.1%, p=0.03). ECG and angiographic indicators of MVO all showed a trend toward worse results in these patients. CONCLUSIONS: The presence of ED assessed by PAT 24 h after P-PCI in patients with STEMI is associated with larger infarcts, lower LVEF, higher WMSI and higher prevalence of MVO.info:eu-repo/semantics/publishedVersio

Il vizio di eccesso di potere: dalla tutela dell'amministrazione alla tutela dall'amministrazione

Dottorato di ricerca in diritto. 7. ciclo. A.a. 1994-95Consiglio Nazionale delle Ricerche - Biblioteca Centrale - P.le Aldo Moro, 7, Rome; Biblioteca Nazionale Centrale - P.za Cavalleggeri, 1, Florence / CNR - Consiglio Nazionale delle RichercheSIGLEITItal

La Passio Callisti e il santuario della Via Aurelia

Giovanni Nino Verrando, La Passio Callisti e il santuario della via Aurelia, p. 1039-1083. In Trastevere, nella seconda metà del IV secolo, era rimasta ben viva una memoria di papa Callisto. Tale memoria, peraltro, non è collegata ad un edificio particolare, tanto meno ad una domus ecclesia, bensì ad un territorio più ampio, ad un'area aperta nell'ambito della quale si era conservato il ricordo del luogo, forse della schola, in cui Callisto aveva a suo tempo operato. La memoria di Callisto era del tutto indipendente dalla basilica Iulii. Tale indipendenza si evidenzia ancora nella Passio Callisti, composta negli ultimi decenni del V secolo. È quindi da questo scritto che ha tratto inspirazione l'autore del Liber pontificalis e non viceversa. Il testo della Passio Callisti ha pure permesso una nuova interpretazione di alcune scene ritrovate nel santuario cimiteriale di papa Callisto al III miglio della via Aurelia.Verrando Giovanni Nino. La Passio Callisti e il santuario della Via Aurelia. In: Mélanges de l'École française de Rome. Antiquité, tome 96, n°2. 1984. pp. 1039-1083

L'innovation dans le projet du GPE : de l'opportunité à la réalisation

L’Innovation est devenue un enjeu majeur pour l’ensemble des décideurs publics dans la déclinaison opérationnelle des politiques publiques. En effet, l’innovation est présentée comme un levier essentiel et efficace pour optimiser la performance, la valeur et les gains engendrés par les projets portés par les autorités publiques. Pour autant, les mégaprojets d’infrastructures publiques, comme le Grand Paris Express (GPE), sont toujours majoritairement conçus selon une conception réglée ou fonctionnelle (loi MOP) et tardent à s’inscrire dans une démarche innovante. Il s’agit donc de mettre en perspective ce constat et d’analyser les actions entreprises par la Société du Grand Paris (SGP), via sa mission Innovation, pour faire évoluer cet état de fait. En effet, au regard de son caractère unique et exceptionnel, le Grand Paris Express est susceptible de faire évoluer les pratiques en faveur de l’innovation et de proposer un nouveau modèle duplicable pour les futurs mégaprojets d’infrastructures

Il santuario di S. Felice sulla via Portuense

Giovanni Nino Verrando, Il santuario di S. Felice sulla via Portuense, p. 331-366. La via Campana, nota dalle fonti anteriori al IV secolo, è la via Portuense, citata dalle fonti posteriori, della quale si accedeva ai santuari paleocristiani. Il santuario di S. Felice, probabilmente, si è inserito nella preesistente basilica di papa Giulio I. Il martire ivi venerato, a partire dell'inizio del VI secolo, è peraltro l'antipapa Felice II, nella sua rinnovata personalità di papa-martire, non un oscuro martire omonimo venuto alla ribalta, sulla base della confusione dei documenti successivi, ad opera degli autori moderni. Il luogo del santuario, mai ritrovato, dovrebbe esser ricercato sulle alture di Vigna Pia, in qualche punto dominante il corso del Tevere. Dovremmo figurarci un edificio subdiale, in quanto il (v. retro) terreno in quei dintorni poco si presta all'escavazione ipogeica, e non può quindi identificarsi con nessuno degli ipogei visti in quella zona nel passato.Verrando Giovanni Nino. Il santuario di S. Felice sulla via Portuense. In: Mélanges de l'École française de Rome. Antiquité, tome 100, n°1. 1988. pp. 331-366