LC-MS analysis and antifungal activity of Turnera subulata Sm.

Fungi of the Candida genus are responsible for invasive candidiasis, which affects people all over the world and has high mortality rates. This is due to their virulence factors, which give them great resistance and pathogenicity. In addition, the emergence of multidrug-resistant strains makes it difficult to treat these infections. In this way, natural products have emerged as an alternative to standard drugs, where plants known for their medicinal properties such as Turnera subulata become attractive to research. The present work aimed to analyze the ethanol extract of Turnera subulata leaves against standard strains of Candida albicans, Candida krusei and Candida tropicalis using broth microdilution techniques. The identification of the compounds in T. subulata leaves by LC-MS revealed the presence of a wide variety of substances such as carboxylic acids and terpenes, with flavonoids and fatty acids being more evident. The antifungal assays showed that the extract was not able to inhibit the growth of the tested strains at concentrations with a clinical relevance. However, at higher concentrations, it was able to inhibit the fungal dimorphism of C. albicans and C. tropicalis. It is possible that the T. subulata extract has potential as an inhibitor of fungal virulence factors without affecting the cell viability. Further research should be carried out in order to assess its inhibitory potential for other fungal virulence factors

Never-lasting Effects: John Williams, Pier Paolo Pasolini, Bruno Jasieński, and Non-redemptive Failure

My dissertation examines literary accounts of failure and failed performance largely in the context of the advent of modernity. I read the works of John Williams, Pier Paolo Pasolini, and Bruno Jasieński as case studies to discuss a non-redemptive kind of failure, one where the narrative does not suggest failing as a step to eventual success. Never-lasting Effectsis an attempt to delineate the productive side of failure within a non-future-oriented approach. In parallel with a reading of the above authors, I review the existing narratives about failure, in the main offered by scholars and critics who see it either as a nihilist, apolitical approach or, in a more hopeful way, as a tool of subversion and revolutionary practice under the conditions of late capitalism. My dissertation carves out a theoretical position outside of either of these opposed camps in what appears to be a nascent field of failure studies. Taking my methodology from performance studies and its emphasis on ephemerality, I examine failure synchronically, as it happens in its present. As I argue, there are important political effects produced by failure that cannot continue into the utopian future. My discussions of Williams, Pasolini, and Jasieński offer a new methodology of reading failure that helps us examine and understand those effects better.Doctor of Philosophy (PhD

Knowledge and attitude of ICU nurses, students and patients towards the Austrian organ donation law

BACKGROUND: A survey on the knowledge and attitudes towards the Austrian organ donation legislation (an opt-out solution) of selected groups of the Austrian population taking into account factors such as age, gender, level of education, affiliation to healthcare professions and health related studies was conducted. METHODS: An online survey among 3 target groups (ICU nurses, health science students and non health science students) was performed and results were compared to the answers from transplantation patients to a paper questionnaire. A total of 8415 persons were asked to participate in the survey and 2025 (24%) persons correctly completed the questionnaire. 1945 online responses (ICU nurses n = 185; students of health sciences n = 1277; students of non-health science related courses n = 483) were analysed and data were compared to 80 manually filled-in responses from patients from a previous study. RESULTS: 84% of participants state that they know the Austrian organ donation legislation; this percentage varies significantly (p < 0.05) within the target groups and is influenced by demographic variables of the participants. 74% think that the law is good and 79% do not favour a change. Opinions and attitudes towards the legal situation are positively influenced by the affiliation to healthcare professions and health-related fields of study. Interviewed persons who were aware of the legislation before the survey had a more positive attitude towards the existing legislation (77% versus 74%, p < 0.05). CONCLUSIONS: The information level on Austrian organ donation legislation is high. ICU nurses and those who did not know the law before were most critical towards the existing legislation. Therefore education to increase knowledge in the general population and goal-oriented efforts to increase awareness in the target groups should be emphasized

MICROSURGICAL TECHNIQUES FOR TRANSPLANTATION OF ORGANS CONTAINING LYMPHOID TISSUE

The technical methods of tr ansplantation of spleen, small bowel and hind extremity in rats used in our laboratory have been presented. Vascularized spleen orthotopic and hetero topic grafts and small bowel transplants are used for studies on spontaneous mi­ gration of isotope labelled cells from and through these organs, hind extremity transplants serve as a source of  live bone marrow  tissue