Retrospective analysis of the treatment of BRCA1 and BRCA2 mutation carriers – the experience of a single-center tertiary institution

INTRODUCTION: Breast cancer gene 1 and 2 (BRCA1/2) mutation carriers are at a higher risk of developing breast cancer. There are several established risk-reducing therapies. Our study aimed to characterize the BRCA1/2 mutation carriers, and to evaluate the implemented treatment methods. MATERIAL AND METHODS: A retrospective analysis of clinical records of 96 female patients hospitalized from October 2019 to December 2022 in the Breast Cancer Unit in Lodz, Poland. RESULTS: Out of 85 BRCA1 and 11 BRCA2 mutation carriers, 96.88% received nipple-sparing or skin-sparing, unilateral or bilateral risk-reducing mastectomies. Out of all the patients, 36 developed 38 breast cancers. One patient was diagnosed with breast cancer 2 years after a bilateral risk-reducing mastectomy. The most common breast cancer subtype was triple-negative breast cancer (73.68%). The patients could receive surgery, chemotherapy, endocrine therapy and radio­therapy. 18 patients had neoadjuvant chemotherapy, in 6 of these patients a complete pathological response (ypT0N0) was achieved. CONCLUSIONS: Oncoplastic bilateral risk-reducing mastectomies are effective and safe procedures

Pengaruh Budaya Organisasi, Komunikasi Interpersonal, dan Perilaku Inovatif, terhadap Motivasi Kerja Guru Sekolah Dasar Negeri di Kabupaten Labuhan Batu

Abstract: The purpose of this research is to determine and analysis: (1) the effect of organizational culture on innovative behavior, (2) the effect of interpersonal communication on innovative behavior, (3) the effect of organizational culture on work motivation, (4) the effect of interpersonal communication on work motivation, and (5) the effect of innovative behavior on work motivation. The research subjects were the teachers of the State Elementary School of Labuhan Batu Regency with a total sample of 142 people taken by means of stratified proportional random sampling, the data were analyzed using path analysis after calculating the correlation of all research variables in the form of a matrix.. The analysis results of the effect exogenous variables on endogenous variables in each substructure obtained (1) organizational culture, interpersonal communication, and innovative behavior have a positive effect on teacher work motivation by 7.7%; 4.7% and 9.7% (2) innovative behavior is influenced by teacher work motivation by 5.3%. The results of the study illustrate that (1) there is a direct positive influence of organizational culture on innovative behavior by 5.24%, (2) there is a direct positive influence of interpersonal communication on innovative behavior of 3.09%, (3) there is a direct positive influence of organizational culture on work motivation, (4) there is an effect of interpersonal communication on work motivation, and (5) there is an influence of innovative behavior on work motivation.. Keyword: Organizational Culture, Interpersonal Communication, Innovative Behavior, and Work Motivation Abstrak: Tujuan penelitian ini adalah untuk mengetahui dan menganalisis: (1) pengaruh budaya organisasi terhadap perilaku inovatif, (2) pengaruh komunikasi interpersonal terhadap perilaku inovatif, (3) pengaruh budaya organisasi terhadap motivasi kerja, (4) pengaruh komunikasi interpersonal terhadap motivasi kerja, dan (5) pengaruh perilaku inovatif terhadap motivasi kerja. Subjek penelitian adalah para guru Sekolah Dasar Negeri Kabupaten Labuhan Batu dengan jumlah sampel sebanyak 283 orang yang diambil dengan cara stratified proportional random sampling, data dianalisis dengan menggunakan analisis jalur setelah dihitung korelasi seluruh variabel penelitian dalam bentuk matriks. Hasil analisis pengaruh variabel eksogen terhadap variabel endogen pada masing-masing substruktur diperoleh (1) budaya organisasi, komunikasi interpersonal, dan perilaku inovatif berpengaruh positif terhadap motivasi kerja guru sebesar 7,7%; 4,7% dan 9,7%  (2) perilaku inovatif dipengaruhi oleh motivasi kerja guru sebesar 5,3%. Hasil penelitian memberikan gambaran bahwa (1) ada pengaruh langsung positif budaya organisasi terhadap perilaku inovatif sebesar 5,24%, (2) ada pengaruh langsung positif komunikasi interpersonal terhadap perilaku inovatif 3,09%, (3) ada pengaruh langsung positif budaya organisasi terhadap motivasi kerja, (4) ada pengaruh komunikasi interpersonal terhadap motivasi kerja, dan (5) ada pengaruh perilaku inovatif terhadap motivasi kerja. Kata Kunci: Budaya Organisasi, Komunikasi Interpersonal, Perilaku Inovatif, dan Motivasi Kerj

Retrospective analysis of the treatment of BRCA1 and BRCA2 mutation carriers – the experience of a single-center tertiary institution

Introduction.Breast cancer gene 1 and 2 (BRCA1/2) mutation carriers are at a higher risk of developing breast cancer. There are several established risk-reducing therapies. Our study aimed to characterize the BRCA1/2 mutation carriers, and to evaluate the implemented treatment methods. Material and methods.A retrospective analysis of clinical records of 96 female patients hospitalized from October 2019 to December 2022 in the Breast Cancer Unit in Lodz, Poland. Results.Out of 85 BRCA1 and 11 BRCA2 mutation carriers, 96.88% received nipple-sparing or skin-sparing, unilateral or bilateral risk-reducing mastectomies. Out of all the patients, 36 developed 38 breast cancers. One patient was diagnosed with breast cancer 2 years after a bilateral risk-reducing mastectomy. The most common breast cancer subtype was triple-negative breast cancer (73.68%). The patients could receive surgery, chemotherapy, endocrine therapy and radio­therapy. 18 patients had neoadjuvant chemotherapy, in 6 of these patients a complete pathological response (ypT0N0) was achieved. Conclusions.Oncoplastic bilateral risk-reducing mastectomies are effective and safe procedures

The molecular portrait of triple-negative breast cancer: the LAG3 gene single nucleotide polymorphism rs2365094 has no impact on the clinical picture

INTRODUCTION: Triple-negative breast cancer (TNBC) is characterized by a lack of oestrogen, progesterone and humanepidermal growth factor receptors. It is the one of most heterogeneous and highly-aggressive breast cancers, resultingin fast progression. In humans, the lymphocyte activation gene 3 (LAG3) is located on chromosome 12p13 and encodesan immune-regulatory molecule. The aim of the study was to perform a molecular analysis of LAG3 gene polymorphisms. MATERIAL AND METHOD: The presence of single-nucleotide polymorphisms (SNPs) at rs2365094 was determined in 30TNBC patients and 30 healthy controls using a polymerase chain reaction (PCR) and commercially-available TaqManSNP Genotyping Assays. SNP status was then compared with the clinical outcome. RESULTS: The allelic alterations in LAG3 gene SNP in rs2365094 appear to have no influence on the clinicopathologicalpicture among TNBC patients. The carriage rate for a single allele did not differ significantly between patients and controls. CONCLUSIONS: No significant relationship was observed between the rs2365094 SNP status and clinicopathologicaldeterminants

The molecular portrait of triple-negative breast cancer: the LAG3 gene single nucleotide polymorphism rs2365094 has no impact on the clinical picture

Introduction. Triple-negative breast cancer (TNBC) is characterized by a lack of estrogen, progesterone and human epidermal growth factor receptors. It is the one of most heterogeneous and highly-aggressive breast cancers, resulting in fast progression. In humans, the lymphocyte activation gene 3 (LAG3) is located on chromosome 12p13 and encodes an immune-regulatory molecule. The aim of the study was to perform a molecular analysis of LAG3 gene polymorphisms. Material and method. The presence of single-nucleotide polymorphisms (SNPs) at rs2365094 was determined in 30 TNBC patients and 30 healthy controls using polymerase chain reaction (PCR) and commercially-available TaqMan SNP Genotyping Assays. SNP status was the compared with clinical outcome. Results. The allelic alterations in LAG3 gene SNP in rs2365094 appear to have no influence on the clinicopathological picture among TNBC patients. The carriage rate for a single allele did not differ significantly between patients and controls. Conclusions. No significant relationship was observed between rs2365094 SNP status and clinicopathological determinants

The molecular portrait of triple-negative breast cancer: the LAG3 gene single nucleotide polymorphism rs2365094 has no impact on the clinical picture

Introduction. Triple-negative breast cancer (TNBC) is characterized by a lack of oestrogen, progesterone and human epidermal growth factor receptors. It is the one of most heterogeneous and highly-aggressive breast cancers, resulting in fast progression. In humans, the lymphocyte activation gene 3 (LAG3) is located on chromosome 12p13 and encodes an immune-regulatory molecule. The aim of the study was to perform a molecular analysis of LAG3 gene polymorphisms.  Material and method. The presence of single-nucleotide polymorphisms (SNPs) at rs2365094 was determined in 30 TNBC patients and 30 healthy controls using a polymerase chain reaction (PCR) and commercially-available TaqMan SNP Genotyping Assays. SNP status was then compared with the clinical outcome.  Results. The allelic alterations in LAG3 gene SNP in rs2365094 appear to have no influence on the clinicopathological picture among TNBC patients. The carriage rate for a single allele did not differ significantly between patients and controls.  Conclusions. No significant relationship was observed between the rs2365094 SNP status and clinicopathological determinants.

Liquid biopsy in targeting gene polymorphism related to the response within immunocheckpoint inhibitors therapeutic regimen

Immunotherapy belongs to the group of targeted therapies; it is based on natural immune mechanisms which axis can be promoted or blocked at appropriate points. Breast cancer is the world’s most common cancer among women and in March 2019 the FDA approved the first immunopharmaceutical Atezolizumab, for the treatment of breast cancer. So far, the only registered marker for classification for checkpoint inhibitor therapy has been the presence of PD-L1 receptor expression in tumour cells. A comprehensive search of the literature to elucidate the correlation between PD-1/PD-L1 single nucleotide polymorphism (SNP) and cancer, especially breast cancer or other diseases susceptibility and PD-1/ PD-L1 expression. Seven susceptibility loci was considered: rs41386349, rs7421861, rs36084323, rs11568821, rs2227981, rs10204525, rs2227982. Three of them may be taken into account as potentially helpful in breast cancer patient treatment tailoring: rs36084323, rs2227981, rs2227982

Immediate breast reconstruction in Poland — description of the protocol for the prospective, multicenter, cohort study PSSO_04 under the auspices of the Polish Society of Surgical Oncology

Breast cancer is the most common cancer among women in Poland, and the number of annual diagnoses is projected to rise. Due to early detection and multidisciplinary treatments, over 60% of cases can undergo breast-conserving procedures. However, mastectomy remains necessary for 30–40% of patients. Immediate breast reconstruction (IBR) following mastectomy provides therapeutic, aesthetic, and psychological benefits, yet the best surgical techniques for IBR are still not well established. The PSSO_04 study is a Polish multicenter research initiative to assess the availability of immediate breast reconstructions (IBR) in therapeutic and prophylactic mastectomies. The study will also evaluate surgical and oncological outcomes, as well as the quality of life, in eligible patients. The study’s primary endpoint is to determine the IBR ratio across different procedures. Secondary endpoints will exa­mine surgical complications, radical excision rates, and their relationship with patient quality of life. Data collection will include surgical techniques, patient demographics, and postoperative outcomes, analyzed using dedicated online tools for comprehensive data management. The Polish Society of Surgical Oncology supports this study, which is expected to conclude by March 2028. The findings are anticipated to enhance breast reconstruction practices and improve patient care in Poland

Retrospective analysis of the treatment of BRCA1 and BRCA2 mutation carriers – the experience of a single-center tertiary institution

Introduction.Breast cancer gene 1 and 2 (BRCA1/2) mutation carriers are at a higher risk of developing breast cancer. There are several established risk-reducing therapies. Our study aimed to characterize the BRCA1/2 mutation carriers, and to evaluate the implemented treatment methods. Material and methods.A retrospective analysis of clinical records of 96 female patients hospitalized from October 2019 to December 2022 in the Breast Cancer Unit in Lodz, Poland. Results.Out of 85 BRCA1 and 11 BRCA2 mutation carriers, 96.88% received nipple-sparing or skin-sparing, unilateral or bilateral risk-reducing mastectomies. Out of all the patients, 36 developed 38 breast cancers. One patient was diagnosed with breast cancer 2 years after a bilateral risk-reducing mastectomy. The most common breast cancer subtype was triple-negative breast cancer (73.68%). The patients could receive surgery, chemotherapy, endocrine therapy and radio­therapy. 18 patients had neoadjuvant chemotherapy, in 6 of these patients a complete pathological response (ypT0N0) was achieved. Conclusions.Oncoplastic bilateral risk-reducing mastectomies are effective and safe procedures