Examining Muscle Activity Differences During Single and Dual Vector Elastic Resistance Exercises

# Background Elastic resistance exercise is a common part of rehabilitation programs. While these exercises are highly prevalent, little information exists on how adding an additional resistance vector with a different direction from the primary vector alters muscle activity of the upper extremity. # Purpose The purpose of this study was to examine the effects of dual vector exercises on torso and upper extremity muscle activity in comparison to traditional single vector techniques. # Study Design Repeated measures design. # Methods Sixteen healthy university-aged males completed four common shoulder exercises against elastic resistance (abduction, flexion, internal rotation, external rotation) while using a single or dual elastic vector at a fixed cadence and standardized elastic elongation. Surface electromyography was collected from 16 muscles of the right upper extremity. Mean, peak and integrated activity were extracted from linear enveloped and normalized data and a 2-way repeated measures ANOVA examined differences between conditions. # Results All independent variables differentially influenced activation. Interactions between single/dual vectors and exercise type affected mean activation in 11/16 muscles, while interactions in peak activation existed in 7/16 muscles. Adding a secondary vector increased activation predominantly in flexion or abduction exercises; little changes existed when adding a second vector in internal and external rotation exercises. The dual vector exercise in abduction significantly increased mean activation in lower trapezius by 25.6 ± 8.11 %MVC and peak activation in supraspinatus by 29.4 ± 5.94 %MVC (p<0.01). Interactions between single/dual vectors and exercise type affected integrated electromyography for most muscles; the majority of these muscles had the highest integrated electromyography in the dual vector abduction condition. # Conclusion Muscle activity often increased with a second resistance vector added; however, the magnitude was exercise-dependent. The majority of these changes existed in the flexion and abduction exercises, with little differences in the internal or external rotation exercises. # Level of Evidence 3

Finding a Needle in the Virus Metagenome Haystack - Micro-Metagenome Analysis Captures a Snapshot of the Diversity of a Bacteriophage Armoire

Viruses are ubiquitous in the oceans and critical components of marine microbial communities, regulating nutrient transfer to higher trophic levels or to the dissolved organic pool through lysis of host cells. Hydrothermal vent systems are oases of biological activity in the deep oceans, for which knowledge of biodiversity and its impact on global ocean biogeochemical cycling is still in its infancy. In order to gain biological insight into viral communities present in hydrothermal vent systems, we developed a method based on deep-sequencing of pulsed field gel electrophoretic bands representing key viral fractions present in seawater within and surrounding a hydrothermal plume derived from Loki's Castle vent field at the Arctic Mid-Ocean Ridge. The reduction in virus community complexity afforded by this novel approach enabled the near-complete reconstruction of a lambda-like phage genome from the virus fraction of the plume. Phylogenetic examination of distinct gene regions in this lambdoid phage genome unveiled diversity at loci encoding superinfection exclusion- and integrase-like proteins. This suggests the importance of fine-tuning lyosgenic conversion as a viral survival strategy, and provides insights into the nature of host-virus and virus-virus interactions, within hydrothermal plumes. By reducing the complexity of the viral community through targeted sequencing of prominent dsDNA viral fractions, this method has selectively mimicked virus dominance approaching that hitherto achieved only through culturing, thus enabling bioinformatic analysis to locate a lambdoid viral “needle" within the greater viral community “haystack". Such targeted analyses have great potential for accelerating the extraction of biological knowledge from diverse and poorly understood environmental viral communities

Examining Muscle Activity Differences During Single and Dual Vector Elastic Resistance Exercises

Background Elastic resistance exercise is a common part of rehabilitation programs. While these exercises are highly prevalent, little information exists on how adding an additional resistance vector with a different direction from the primary vector alters muscle activity of the upper extremity. Purpose The purpose of this study was to examine the effects of dual vector exercises on torso and upper extremity muscle activity in comparison to traditional single vector techniques. Study Design Repeated measures design. Methods Sixteen healthy university-aged males completed four common shoulder exercises against elastic resistance (abduction, flexion, internal rotation, external rotation) while using a single or dual elastic vector at a fixed cadence and standardized elastic elongation. Surface electromyography was collected from 16 muscles of the right upper extremity. Mean, peak and integrated activity were extracted from linear enveloped and normalized data and a 2-way repeated measures ANOVA examined differences between conditions. Results All independent variables differentially influenced activation. Interactions between single/dual vectors and exercise type affected mean activation in 11/16 muscles, while interactions in peak activation existed in 7/16 muscles. Adding a secondary vector increased activation predominantly in flexion or abduction exercises; little changes existed when adding a second vector in internal and external rotation exercises. The dual vector exercise in abduction significantly increased mean activation in lower trapezius by 25.6 ± 8.11 %MVC and peak activation in supraspinatus by 29.4 ± 5.94 %MVC (p&lt;0.01). Interactions between single/dual vectors and exercise type affected integrated electromyography for most muscles; the majority of these muscles had the highest integrated electromyography in the dual vector abduction condition. Conclusion Muscle activity often increased with a second resistance vector added; however, the magnitude was exercise-dependent. The majority of these changes existed in the flexion and abduction exercises, with little differences in the internal or external rotation exercises. Level of Evidence 3b </jats:sec