Comparison of glycated hempglobin with HPLC and capillary electrophoresis

Background: Hemoglobin A1c, also called A1c or glycated hemoglobin, is hemoglobin with glucose attached. The A1c test evaluates the average amount of glucose in the blood over the last 2 to 3 months. The higher the level of glucose in the blood, the more glycated hemoglobin is formed. Once the glucose binds to the hemoglobin, it remains there for the life of the red blood cell – normally about 120 days. The predominant form of glycated hemoglobin is referred to as A1c. Testing of HbA1c levels via capillary electrophoresis is a relatively new but well-validated method that separates A1c and other Hb fractions via charge difference at high voltage using electro-osmotic flow. This method can be useful in patients who possess such variant hemoglobins because it has a longer runtime, leading to better resolution.Methods: We have processed random samples coming to our laboratory for HbA1C analysis on both the analyzers Biorad D 10 (HPLC method) and Sebia Flex piercing (Capillary Electrophoresis).Results: The value of t is 0.056748 for paired 't' test. The value of p is 0.954819. The result is not significant at p≤0.05. There is no significant difference between the results obtained from both the equipment.Conclusions: From this study, it is concluded that the results obtained after testing samples in Sebia Flex Piercing II and Biorad D10 are comparable and there is no significant difference in the results obtained. The advantage of of using Sebia is detection of underlying hemoglobinopathies is easier and can serve as passive surveillance in population that will provide additional information for multidisciplinary approach of treatment. Whereas Biorad D10 has benefit of shorter testing time and is cost effective

Study of glucose specimen integrity in various blood collection tubes over a period of 24 hours

Background: Blood glucose testing is performed to diagnose and monitor conditions such as diabetes mellitus and gestational diabetes in pregnant women. Accurate measurement of plasma glucose concentration is an important component. Here, we have compared the glucose estimation in specimen collected in various collection tubes. Methods: The samples were stored at 2-4°C temperature and at 0, 4, 12 and 24 hrs aliquots were taken and were processed. Plasma glucose was determined using the glucose oxidase enzymatic methods on the Beckman coulter AU700. All the results at 0 hour were compared with results at 4 hours, 12 hours and 24 hours. Also results of fluoride tubes were compared with results of EDTA, heparin and plane tubes. Results: We have found no significant difference between results of glucose estimation obtained using samples collected in serum tubes, EDTA tubes, fluoride tube and lithium heparin tube even after 24 hours after separation of serum/plasma. Conclusions: From this study we have concluded that blood samples collected in plane serum tube, fluoride tubes, EDTA tubes and heparin tubes produce similar results for estimation of glucose if the collected samples are centrifuged immediately after collection for separation of plasma and serum

A study to assess cardiac diseases in pregnancy and pregnancy outcome

Background: Heart disease has a significant impact on fetal and maternal health during pregnancy, labor and delivery. In India and other developing countries, rheumatic heart disease is most common. Cardiac disorders are observed in approximately 1% of pregnancies; they account for morbidity and mortality rates. The objective of the study was to analyze the effect of cardiac diseases on maternal and fetal outcome.Methods: Hospital based cohort study was done for a period of 5 years. All pregnant women with cardiac disease were the study participants. Total numbers of women with cardiac disease were 390.Results: It was observed that majority of the study participants were suffering with mitral stenois (30%), followed by mitral regurgitation (25%) and mitral strenosis and regurgitation (12%). Very few women died because of certain complications, atrial fibrillation (1%), congestive cardiac failure (1%) and pulmonary embolism (1%). The overall perinatal mortality was found to be 4% in the present study.Conclusions: Proper screening of the pregnant women to rule out any cardiac disease is required. If timely intervention are taken to treat the pregnant women with cardiac disease the morbidity and mortality in mother and foetus can be prevented to a great extent

Application of DTM Method for Solving Electrical Engineering Problems of Simple Electric Circuits

In this paper Chou�s Method (DTM) for solving initial valve problems involving first order ordinary differential quotations we introduce the concept of DTM & applied it to obtain solution of three examples for demonstration. The results are compare with exact solution & DTM solutions

Sessile solitary osteochondroma at dorsal scapula: a case report

Osteochondroma is the most common benign tumor of the bone that occurs predominantly in metaphyseal regions of the long bones but rarely involves flat bones like scapula. However, this is often the most common primary benign bone tumor affecting scapula. These tumors usually occur in the growing age and cease to extend in size after skeletal maturity. Any increase in swelling of an asymptomatic swelling turning symptomatic should raise the suspicion of a malignancy. Here, we presented a case of swelling over the left scapula of a fourteen-year-old boy by his parents since the past three years, which showed no progress since last 1 year but however they presented to the hospital for cosmetic reasons. We performed an open extra periosteal resection of the osteochondroma using modified Judet’s approach after diagnostic workup and ruling out malignancy and confirmation by post operative histopathological study of resected lesion. Patient was able to perform full range of motion at his left shoulder after 1 month. With no evidence of recurrence even after six months of follow-up post operatively

The accessibility and development impacts of new transit infrastructure : the Circle Line in Chicago

Thesis (M.C.P.)--Massachusetts Institute of Technology, Dept. of Urban Studies and Planning; and, (S.M. in Transportation)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2007.Includes bibliographical references (p. 213-216).This thesis explores the impacts of new transit infrastructure projects on the land use transportation system. Land use and transportation systems are inherently interconnected and form an integrated system; and transport projects affect not just the transportation system, but also land use patterns. Current evaluation frameworks and models applied to new transport infrastructure projects usually focus on transportation system improvements, and so do not reflect the process of land use transportation interaction. To overcome this limitation, this thesis argues that the evaluation of transport projects should consider their accessibility and development impacts; and that this requires models which explicitly incorporate the interaction between land use and transportation. The Circle Line in Chicago is a proposed circumferential rail transit line which will serve the urban core of the Chicago region, and is likely to significantly affect both land use and travel patterns.(cont.) This thesis assesses how existing models can be used to quantify the accessibility and development impacts of this project; and how an existing evaluation framework, developed by the Federal Transit Administration, considers these impacts. In addition, this thesis develops a quasi-integrated land use transportation model for the Chicago region; and applies it to quantify the accessibility and development impacts of the Circle Line project. The frameworks and models developed for the Circle Line project provide an example of how to improve the current FTA evaluation methodology.by Ritesh K. Warade.S.M.in TransportationM.C.P

Brodie’s abscess of proximal shaft radius in 17-year-old male: a case report

Brodies abscess is an uncommon form of osteomyelitis. It is typically found in metaphyseal region of long bones particularly of lower limb. We herein report rare case of Brodies abscess of proximal shaft radius in 17-year-old male presented with pain over proximal aspect of left forearm for 2 months. A plain radiograph showed a translucent lesion of approximately 1.9×1.1 cm. Magnetic resonance imaging (MRI) showed Penumbra sign on T1 and T2 weighted images. Laboratory results showed no inflammatory response. Treatment was done by curettage. Culture report showed Staphylococcus aureus growth. Postoperatively administration of antibiotics was done. Follow up was uneventful. We diagnosed and surgically treated a rare case of Brodies abscess of proximal shaft radius in 17-year-old male. As the upper extremities are areas of unloaded bone, we successfully treated this patient by curettage and antibiotics without bone grafting.

Effect of nitrogen levels on growth and yield of turmeric

An experiment was conducted during 2000-2001 to study the response of two turmeric varieties (Krishna and Selam), five levels of nitrogen (0, 40, 80, 120, 150 kg ha-1) on growth and yield. The results indicated that variety Krishna responded favourably for all the characters to the application of 120 kg N ha-1. The maximum rhizome yield of 277.87 and 225.43 q ha-1, respectively was harvested by the application of 120 kg N ha-1 with turmeric variety 'Krishna'. The interaction of nitrogen x variety was significant only for fresh weight of fingers per plant. &nbsp