Simulation of Fire Occurrence Based on Historical Data in Future Climate Scenarios and Its Practical Verification

Forest fire is one of the dominant disturbances in the forests of Heilongjiang Province, China, and is one of the most rapid response predictors that indicate the impact of climate change on forests. This study calculated the Canadian FWI (Fire Weather Index) and its components from meteorological record over past years, and a linear model was built from the monthly mean FWI and monthly fire numbers. The significance test showed that fire numbers and FWI had a very pronounced correlation, and monthly mean FWI was suitable for predicting the monthly fire numbers in this region. Then FWI and its components were calculated from the SRES (IPCC Special Report on Emission Scenarios) A2 and B2 climatic scenarios, and the linear model was rebuilt to be suitable for the climatic scenarios. The results indicated that fire numbers would increase by 2.98–129.97% and −2.86–103.30% in the A2 and B2 climatic scenarios during 2020–2090, respectively. The monthly variation tendency of the FWI components is similar in the A2 and B2 climatic scenarios. The increasing fire risk is uneven across months in these two climatic scenarios. The monthly analysis showed that the FFMC (Fine Fuel Moisture Code) would increase dramatically in summer, and the decreasing precipitation in summer would contribute greatly to this tendency. The FWI would increase rapidly from the spring fire season to the autumn fire season, and the FWI would have the most rapid increase in speed in the spring fire season. DMC (Duff Moisture Code) and DC (Drought Code) have relatively balanced rates of increasing from spring to autumn. The change in the FWI in this region is uneven in space as well. In early 21st century, the FWI of the north of Heilongjiang Province would increase more rapidly than the south, whereas the FWI of the middle and south of Heilongjiang Province would gradually catch up with the increasing speed of the north from the middle of 21st century. The changes in the FWI across seasons and space would influence the fire management policy in this region, and the increasing fire numbers and variations in the FWI scross season and space suggest that suitable development of the management of fire sources and forest fuel should be conducted

Investigating the Latency of Lightning-Caused Fires in Boreal Coniferous Forests Using Random Forest Methodology

This study investigates the latency of lightning-caused fires in the boreal coniferous forests of the Greater Khingan Mountains, employing advanced machine learning techniques to analyze the relationship between meteorological factors, lightning characteristics, and fire ignition and smoldering processes. Using the Random Forest Model (RFM) combined with Recursive Feature Elimination with Cross-Validation (RFECV) and SHapley Additive exPlanations (SHAP), the study identifies key factors influencing fire latency. Two methods, Min distance and Min latency, were used to determine ignition lightning, with the Min distance method proving more reliable. The results show that lightning-caused fires cluster spatially and peak temporally between May and July, aligning with lightning activity. The Fine Fuel Moisture Code (FFMC) and precipitation were identified as the most influential factors. This study underscores the importance of fuel moisture and weather conditions in determining latency of lightning-caused fire, offering valuable insights for enhancing early warning systems. Despite limitations in data resolution and the exclusion of topographic factors, this study advances our understanding of lightning-fire latency mechanisms and provides a foundation for more effective wildfire management strategies under climate change

Immunization with Heat Shock Protein A and γ-Glutamyl Transpeptidase Induces Reduction on the Helicobacter pylori Colonization in Mice.

The human gastric pathogen Helicobacter pylori (H. pylori) is a successful colonizer of the stomach. H. pylori infection strongly correlates with the development and progression of chronic gastritis, peptic ulcer disease, and gastric malignances. Vaccination is a promising strategy for preventing H. pylori infection. In this study, we evaluated the candidate antigens heat shock protein A (HspA) and H. pylori γ-glutamyl transpeptidase (GGT) for their effectiveness in development of subunit vaccines against H. pylori infection. rHspA, rGGT, and rHspA-GGT, a fusion protein based on HspA and GGT, were constructed and separately expressed in Escherichia coli and purified. Mice were then immunized intranasally with these proteins, with or without adjuvant. Immunized mice exhibited reduced bacterial colonization in stomach. The highest reduction in bacterial colonization was seen in mice immunized with the fusion protein rHspA-GGT when paired with the mucosal adjuvant LTB. Protection against H. pylori colonization was mediated by a strong systemic and localized humoral immune response, as well as a balanced Th1/Th2 cytokine response. In addition, immunofluorescence microscopy confirmed that rHspA-GGT specific rabbit antibodies were able to directly bind H. pylori in vitro. These results suggest antibodies are essential to the protective immunity associated with rHspA-GGT immunization. In summary, our results suggest HspA and GGT are promising vaccine candidates for protection against H. pylori infection

Photosynthetic contribution and characteristics of cucumber stem and petiole

Abstract Background Photosynthesis of plant non-leaf blade green tissue has been studied in some plants, but the photosynthesis characteristics of stem and petiole are poorly understood. Cucurbitaceous plants are climbing plants, and have a large biomass of stem and petiole. Understanding the photosynthetic contribution of cucumber stem and petiole to growth and the underlying molecular mechanisms are important for the regulation of growth in cucumber production. Results Here, the photosynthetic capacity of cucumber stem and petiole were proved by 14CO2 uptake. The total carbon fixation of stem and petioles is around 4% to that of one leaf blade in cucumber seedling stage, while the proportion of carbon accumulated in stem and petioles redistributed to sink organs (root and growing point) is increased obviously under leaf less condition. Photosynthetic properties of cucumber stem and petiole were studied using a combination of electron microscopy, chlorophyll fluorescence imaging and isotope tracer to compare with leaf blade using two genotype of cucumber (dark green and light green stems). Compare with leaf blade, chlorophyll contents of cucumber stem and petiole are lower, and accompanying with lower chloroplast number, lower stoma number, but with higher thylakoid grana lamella number and larger stomata size. The total photosynthetic rate of stem and petiole is equivalent to 6 ~ 8% of one leaf blade, but the respiration rates were simiar in all the three tissues, which shown an almost 0 net photosynthetic rate in stems and petioles, and with lower non-photochemical quenching (NPQ). Transcriptome analysis showed that compared with leaf blade, there are significantly different gene expressions in photosynthesis, porphyrin and chlorophyll metabolism, photosynthetic antenna proteins and carbon fixation in stem and petiole. Although with lower Rubisco expression level in stem and petiole, Rubisco and PEPC enzyme activities were both higher in stem and petiole than in leaf blade, suggesting the photosynthetic and respiratory mechanisms in stem and petiole are different from those in leaf blade. Conclusions In this study, we confirmed the photosynthetic contribution to growth of cucumber stem and petiole, and shown their similar photosynthetic pattern in tissue anatomy, molecular biology and physiology.</jats:p

Photosynthetic contribution and characteristics of cucumber stems and petioles

Abstract Background Photosynthesis in the green leafless blade tissues or organs of plants has been studied in some plants, but the photosynthetic characteristics of stems and petioles are poorly understood. Cucurbitaceous plants are climbing plants that have substantial stem and petiole biomass. Understanding the photosynthetic contribution of cucumber stems and petioles to their growth and the underlying molecular mechanisms are important for the regulating of growth in cucumber production. Results In this study, the photosynthetic capacity of cucumber stems and petioles were determined by 14CO2 uptake. The total carbon fixed by the stems and petioles was approximately 4% of that fixed by one leaf blade in the cucumber seedling stage, while the proportion of the carbon accumulated in the stems and petioles that redistributed to sink organs (roots and shoot apexes) obviously increased under leafless conditions. The photosynthetic properties of cucumber stems and petioles were studied using a combination of electron microscopy and isotope tracers to compare these properties of stems and petioles with those of leaf blade using two genotypes of cucumber (dark green and light green). Compared with those of the leaf blades, the chlorophyll contents of the cucumber stems and petioles were lower, and the stems and petioles had lower chloroplast numbers and lower stoma numbers but higher thylakoid grana lamella numbers and larger stoma sizes. The Chl a/b ratios were also decreased in the petioles and stems compared with those in the leaf blades. The total photosynthetic rates of the stems and petioles were equivalent to 6 ~ 8% of that of one leaf blade, but the respiration rates were similar in all the three organs, with an almost net 0 photosynthetic rate in the stems and petioles. Transcriptome analysis showed that compared with the leaf blades, the stems and petioles has significantly different gene expression levels in photosynthesis, porphyrin and chlorophyll metabolism; photosynthetic antenna proteins; and carbon fixation. PEPC enzyme activities were higher in the stems and petioles than in the leaf blades, suggesting that the photosynthetic and respiratory mechanisms in stems and petioles are different from those in leaf blade, and these results are consistent with the gene expression data. Conclusions In this study, we confirmed the photosynthetic contribution to the growth of cucumber stems and petioles, and showed their similar photosynthetic patterns in the terms of anatomy, molecular biology and physiology, which were different from those of cucumber leaf blades. </jats:sec

Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate.

Human cytomegalovirus (HCMV) enters host by glycoprotein B (gB)-mediated membrane fusion upon receptor-binding to gH/gL-related complexes, causing devastating diseases such as birth defects. Although an X-ray crystal structure of the recombinant gB ectodomain at postfusion conformation is available, the structures of prefusion gB and its complex with gH/gL on the viral envelope remain elusive. Here, we demonstrate the utility of cryo electron tomography (cryoET) with energy filtering and the cutting-edge technologies of Volta phase plate (VPP) and direct electron-counting detection to capture metastable prefusion viral fusion proteins and report the structures of glycoproteins in the native environment of HCMV virions. We established the validity of our approach by obtaining cryoET in situ structures of the vesicular stomatitis virus (VSV) glycoprotein G trimer (171 kD) in prefusion and postfusion conformations, which agree with the known crystal structures of purified G trimers in both conformations. The excellent contrast afforded by these technologies has enabled us to identify gB trimers (303kD) in two distinct conformations in HCMV tomograms and obtain their in situ structures at up to 21 Å resolution through subtomographic averaging. The predominant conformation (79%), which we designate as gB prefusion conformation, fashions a globular endodomain and a Christmas tree-shaped ectodomain, while the minority conformation (21%) has a columnar tree-shaped ectodomain that matches the crystal structure of the "postfusion" gB ectodomain. We also observed prefusion gB in complex with an "L"-shaped density attributed to the gH/gL complex. Integration of these structures of HCMV glycoproteins in multiple functional states and oligomeric forms with existing biochemical data and domain organization of other class III viral fusion proteins suggests that gH/gL receptor-binding triggers conformational changes of gB endodomain, which in turn triggers two essential steps to actuate virus-cell membrane fusion: exposure of gB fusion loops and unfurling of gB ectodomain

<i>H</i>. <i>pylori</i> colonization in mouse stomachs after immunization.

<p>BALB/c mice (n = 10) were immunized intranasally on days 0, 14 and 21 with 30 μg antigen, with or without 10μg adjuvant, as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130391#pone.0130391.t001" target="_blank">Table 1</a>. The same volume of PBS was used as a negative control. Three weeks after the final vaccination boost, mice were orally challenged four times with <i>H</i>. <i>pylori</i> B6. The level of gastric <i>H</i>. <i>pylori</i> colonization was determined by real-time quantitative PCR four weeks post challenge for each mouse. Data are expressed as mean ± S.D. Significant differences between indicated groups are presented as P values.</p

Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate.

Human cytomegalovirus (HCMV) enters host by glycoprotein B (gB)-mediated membrane fusion upon receptor-binding to gH/gL-related complexes, causing devastating diseases such as birth defects. Although an X-ray crystal structure of the recombinant gB ectodomain at postfusion conformation is available, the structures of prefusion gB and its complex with gH/gL on the viral envelope remain elusive. Here, we demonstrate the utility of cryo electron tomography (cryoET) with energy filtering and the cutting-edge technologies of Volta phase plate (VPP) and direct electron-counting detection to capture metastable prefusion viral fusion proteins and report the structures of glycoproteins in the native environment of HCMV virions. We established the validity of our approach by obtaining cryoET in situ structures of the vesicular stomatitis virus (VSV) glycoprotein G trimer (171 kD) in prefusion and postfusion conformations, which agree with the known crystal structures of purified G trimers in both conformations. The excellent contrast afforded by these technologies has enabled us to identify gB trimers (303kD) in two distinct conformations in HCMV tomograms and obtain their in situ structures at up to 21 Å resolution through subtomographic averaging. The predominant conformation (79%), which we designate as gB prefusion conformation, fashions a globular endodomain and a Christmas tree-shaped ectodomain, while the minority conformation (21%) has a columnar tree-shaped ectodomain that matches the crystal structure of the "postfusion" gB ectodomain. We also observed prefusion gB in complex with an "L"-shaped density attributed to the gH/gL complex. Integration of these structures of HCMV glycoproteins in multiple functional states and oligomeric forms with existing biochemical data and domain organization of other class III viral fusion proteins suggests that gH/gL receptor-binding triggers conformational changes of gB endodomain, which in turn triggers two essential steps to actuate virus-cell membrane fusion: exposure of gB fusion loops and unfurling of gB ectodomain

Vaccine formulations in each group.

<p>Vaccine formulations in each group.</p