Design and Characterisation of Blue Polymer Lasers

Semiconducting polymers have attracted considerable attention as novel gain materials for laser devices. An important future target in this context is the realisation of a thin- film polymer laser diode. Since inorganic semiconductors are amongst the most important devices in modern optoelectronic technology, there is a lot of interest in achieving electrically pumped laser action in organic semiconductors as a way to broadly tunable lasers covering the whole visible spectrum and producing low-cost laser sources for optical networks. This thesis reports the results of a study on the design and characterisation of optically pumped blue and violet emitting polymer lasers. The laser devices are based on a range of materials belonging to the polyfluorene family of conjugated polymers which generally show efficient, low threshold stimulated emission. For future electrically pumped polymer lasers, a further reduction of the threshold is crucial since a low threshold fluence directly translates into low current densities. The optical properties of in total three polyfluorene copolymers are investigated. Lasers based on one of these copolymers are optically-pumped and emission wavelength tuning is demonstrated by altering both grating period and gain polymer thickness, allowing us to cover a part of the spectral region between the blue and ultra-violet that has not been addressed yet by organic semiconductor lasers. Furthermore, a systematic numerical study of the optical environment on the performance of blue emitting lasers on conducting DFB resonators is presented, which is followed by a demonstration of optically-pumped polymer lasers based on ITO gratings. Finally, the results of a systematic study into optically pumped blue emitting polymer lasers based on circular Bragg (CBR) resonators is reported. An optimised design strategy is implemented and involves matching the grating pro files with the nulls and maxima from the Bessel functions that represent the radial distribution of the fi eld in a circular resonator

In vivo mapping of nucleosomes using psoralen-DNA crosslinking and primer extension

By the use of psoralen crosslinking and primer extension, a method was developed which allows the analysis of chromatin structure in vivo. Using a yeast minichromosome, >9 nucleosomes were mapped with a resolution of at least ±30 b

Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system

DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNA:DNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop–mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis

Sumoylation of Smc5 Promotes Error-free Bypass at Damaged Replication Forks

Replication of a damaged DNA template can threaten the integrity of the genome, requiring the use of various mechanisms to tolerate DNA lesions. The Smc5/6 complex, together with the Nse2/Mms21 SUMO ligase, plays essential roles in genome stability through undefined tasks at damaged replication forks. Various subunits within the Smc5/6 complex are substrates of Nse2, but we currently do not know the role of these modifications. Here we show that sumoylation of Smc5 is targeted to its coiled-coil domain, is upregulated by replication fork damage, and participates in bypass of DNA lesions. smc5-KR mutant cells display defects in formation of sister chromatid junctions and higher translesion synthesis. Also, we provide evidence indicating that Smc5 sumoylation modulates Mph1-dependent fork regression, acting synergistically with other pathways to promote chromosome disjunction. We propose that sumoylation of Smc5 enhances physical remodeling of damaged forks, avoiding the use of a more mutagenic tolerance pathway.Ministerio de Ciencia, Innovacion y Universidades (BFU2015-71308-P, PGC2018-097796-B-I00)AGAUR-Generalitat de Catalunya (2017-SGR-569

Energy balance of biogas production from microalgae: Effect of harvesting method, multiple raceways, scale of plant and combined heat and power generation

A previously-developed mechanistic energy balance model for production of biogas from the anaerobic digestion of microalgal biomass grown in open raceway systems was used to consider the energetic viability of a number of scenarios, and to explore some of the most critical parameters affecting net energy production. The output demonstrated that no single harvesting method of those considered (centrifugation, settlement or flocculation) produced an energy output sufficiently greater than operational energy inputs to make microalgal biogas production energetically viable. Combinations of harvesting methods could produce energy outputs 2.3–3.4 times greater than the operational energy inputs. Electrical energy to power pumps, mixers and harvesting systems was 5–8 times greater than the heating energy requirement. If the energy to power the plant is generated locally in a combined heat and power unit, a considerable amount of ‘low grade’ heat will be available that is not required by the process, and for the system to show a net operational energy return this must be exploited. It is concluded that the production of microalgal biogas may be energetically viable, but it is dependent on the effective use of the heat generated by the combustion of biogas in combined heat and power units to show an operational energy retur

Human heterochromatin protein 1 isoforms HP1(Hsα) and HP1(Hsβ) interfere with hTERT-telomere interactions and correlate with changes in cell growth and response to ionizing radiation

Telomeres are associated with the nuclear matrix and are thought to be heterochromatic. We show here that in human cells the overexpression of green fluorescent protein-tagged heterochromatin protein 1 (GFP-HP1) or nontagged HP1 isoforms HP1(Hsα) or HP1(Hsβ), but not HP1(Hsγ), results in decreased association of a catalytic unit of telomerase (hTERT) with telomeres. However, reduction of the G overhangs and overall telomere sizes was found in cells overexpressing any of these three proteins. Cells overexpressing HP1(Hsα) or HP1(Hsβ) also display a higher frequency of chromosome end-to-end associations and spontaneous chromosomal damage than the parental cells. None of these effects were observed in cells expressing mutants of GFP-ΔHP1(Hsα), GFP-ΔHP1(Hsβ), or GFP-ΔHP1(Hsγ) that had their chromodomains deleted. An increase in the cell population doubling time and higher sensitivity to cell killing by ionizing radiation (IR) treatment was also observed for cells overexpressing HP1(Hsα) or HP1(Hsβ). In contrast, cells expressing mutant GFP-ΔHP1(Hsα) or GFP-ΔHP1(Hsβ) showed a decrease in population doubling time and decreased sensitivity to IR compared to the parental cells. The effects on cell doubling times were paralleled by effects on tumorigenicity in mice: overexpression of HP1(Hsα) or HP1(Hsβ) suppressed tumorigenicity, whereas expression of mutant HP1(Hsα) or HP1(Hsβ) did not. Collectively, the results show that human cells are exquisitely sensitive to the amount of HP1(Hsα) or HP1(Hsβ) present, as their overexpression influences telomere stability, population doubling time, radioresistance, and tumorigenicity in a mouse xenograft model. In addition, the isoform-specific effects on telomeres reinforce the notion that telomeres are in a heterochromatinized state

Easy and green route towards nanostructured ZnO as active sensing material with unexpected H2S dosimeter-type behaviour

Nanostructured ZnO particles were prepared through a straightforward, quick and low\u2010temperature synthesis route involving coprecipitation of the metal precursor salts with oxalic acid, followed by hydrothermal treatment at 135 or 160 \ub0C. The synthesised nanostructured powders were thoroughly characterised by a wide array of analytical techniques from the morphological (Scanning Electron Microscopy \u2013SEM\u2010, Transmission Electron Microscopy \u2010TEM\u2010, Energy\u2010dispersive X\u2010ray Spectroscopy \u2010EDXS\u2010), structural (Powder X\u2010Ray Diffraction \u2010PXRD\u2010, Selected Area Electron Diffraction \u2010SAED\u2010), compositional (X\u2010ray Photoelectron Spectroscopy \u2010XPS\u2010) and physical (thermal stability) point of view. As far as functional applications are concerned, the powders were tested as gas sensor materials for H2S detection. Thereby these ZnO particles show unexpected gas dosimeter behaviour at 150 \ub0C. Based on these observations and on a comparison with literature a new model for the interaction of ZnO nanostructures with H2S is proposed

Non‐Canonical Replication Initiation: You’re Fired!

The division of prokaryotic and eukaryotic cells produces two cells that inherit a perfect copy of the genetic material originally derived from the mother cell. The initiation of canonical DNA replication must be coordinated to the cell cycle to ensure the accuracy of genome duplication. Controlled replication initiation depends on a complex interplay of cis‐acting DNA sequences, the so‐called origins of replication (ori), with trans‐acting factors involved in the onset of DNA synthesis. The interplay of cis‐acting elements and trans‐acting factors ensures that cells initiate replication at sequence‐specific sites only once, and in a timely order, to avoid chromosomal endoreplication. However, chromosome breakage and excessive RNA:DNA hybrid formation can cause breakinduced (BIR) or transcription‐initiated replication (TIR), respectively. These non‐canonical replication events are expected to affect eukaryotic genome function and maintenance, and could be important for genome evolution and disease development. In this review, we describe the difference between canonical and non‐canonical DNA replication, and focus on mechanistic differences and common features between BIR and TIR. Finally, we discuss open issues on the factors and molecular mechanisms involved in TIR.España, MINECO BFU2015-69183-

Yeast as a model system to study metabolic impact of selenium compounds

Inorganic Se forms such as selenate or selenite (the two more abundant forms in nature) can be toxic in Saccharomyces cerevisiae cells, which constitute an adequate model to study such toxicity at the molecular level and the functions participating in protection against Se compounds. Those Se forms enter the yeast cell through other oxyanion transporters. Once inside the cell, inorganic Se forms may be converted into selenide through a reductive pathway that in physiological conditions involves reduced glutathione with its consequent oxidation into diglutathione and alteration of the cellular redox buffering capacity. Selenide can subsequently be converted by molecular oxygen into elemental Se, with production of superoxide anions and other reactive oxygen species. Overall, these events result in DNA damage and dose-dependent reversible or irreversible protein oxidation, although additional oxidation of other cellular macromolecules cannot be discarded. Stress-adaptation pathways are essential for efficient Se detoxification, while activation of DNA damage checkpoint and repair pathways protects against Se-mediated genotoxicity. We propose that yeast may be used to improve our knowledge on the impact of Se on metal homeostasis, the identification of Se-targets at the DNA and protein levels, and to gain more insights into the mechanism of Se-mediated apoptosis.España, Junta de Andalucía P11-CTS- 796