Hydroiden von Amboina und Thursday Islands

Die von Herrn Professor Semon bei seinem Besuche der Thursday-Insel und Ambon 1892-1893 gesammelten Hydroidpolypen setzten sich aus 12 Arten zusammen, darunter 5 aus Ambon und 7 von der Thursday-Insel. Eine davon ist neu (Acanthocladium studeri), eine andere Art konnte mangels der Gonotheken nicht näher bestimmt werden. Die Hydroidenfauna Ambons hat PICTET (93) während eines zweimonatlichen Aufentlialtes eingehend studirt und 32 Arten von dort bekannt gemacht, PICTHT hebt die Armuth Ambons an litoralen Hydroiden gegenüber den übrigen dort lebenden Evertebraten hervor. Die wenigen (4) Arten, welche SEMON von hier mitgebracht hat, hat auch PICTET dort beobachtet und beschrieben. Folgende Species sind bei Ambon und der Thursday-lnsel von Herrn Professor SEMON erbeutet worden: ..

Prismane C_8: A New Form of Carbon?

Our numerical calculations on small carbon clusters point to the existence of a metastable three-dimensional eight-atom cluster C$_8$ which has a shape of a six-atom triangular prism with two excess atoms above and below its bases. We gave this cluster the name "prismane". The binding energy of the prismane equals to 5.1 eV/atom, i.e., is 0.45 eV/atom lower than the binding energy of the stable one-dimensional eight-atom cluster and 2.3 eV/atom lower than the binding energy of the bulk graphite or diamond. Molecular dynamics simulations give evidence for a rather high stability of the prismane, the activation energy for a prismane decay being about 0.8 eV. The prismane lifetime increases rapidly as the temperature decreases indicating a possibility of experimental observation of this cluster.Comment: 5 pages (revtex), 3 figures (eps

Generation of a SOX2 reporter human induced pluripotent stem cell line using CRISPR/SaCas9

SOX2 is an important transcription factor involved in pluripotency maintenance, pluripotent reprogramming and differentiation towards neural lineages. Here we engineered the previously described HEL24.3 hiPSC to generate a SOX2 reporter by knocking-in a T2A fused nuclear tdTomato reporter cassette before the STOP codon of the SOX2 gene coding sequence. CRISPR/SaCas9-mediated stimulation of homologous recombination was utilized to facilitate faithful targeted insertion. This line accurately reports the expression of endogenous SOX2 and therefore constitutes a useful tool to study the SOX2 expression dynamics upon hiPSC culture, differentiation and somatic cell reprogramming. (C) 2017 The Authors. Published by Elsevier B.V.Non peer reviewe

Solution Synthesis and Additive Manufacturing of Bismuth Telluride Nanoflakes for Flexible Energy Harvesting

Flexible thermoelectric generators (TEGs) are energy harvesting devices which convert temperature differences into electrical power. These devices require no moving parts and offer silent and autonomous operation. The variety of suitable materials covering a broad range of operating temperatures positions TEGs as a promising renewable energy source using waste heat recovery, especially for space and microgravity applications. Conventional manufacturing of flexible electronic and thermoelectric devices requires complicated and relatively expensive processing, which limits the capabilities of in-space manufacturing. Additive manufacturing (AM) expands the use of flexible electronics to new surfaces, applications, and recently, low gravity conditions. Plasma jet printing (PJP) is a new AM modality in which material is deposited via a gravity- independent plasma. This thesis demonstrates solution processing, ink synthesis, and PJP of bismuth telluride (Bi2Te3) nanoflakes for low temperature energy harvesting. Synthesis conditions were tailored to control nanoflake morphology and ink processing was optimized for direct printing on flexible polyimide substrates. The thermoelectric films demonstrate promising thermoelectric properties, material adhesion, and flexibility, with only a 7.2% variation in performance after 10,000 bending cycles over a 16 mm radius of curvature. This advancement of Bi2Te3 solution processing and demonstration of PJP of thermoelectric films marks a significant contribution to in space manufacturing of flexible thermoelectric devices for wearable technology and low temperature energy harvesting

Verbal Abuse in Romantic Relationships

In this qualitative study, six participants aged 19 to 71 were interviewed regarding the evolution between past verbally abusive relationships to their current romantic relationship. The findings showed that verbal abuse is destructive and can undermine one\u27s self-worth. It also determined that reflecting on one\u27s experience can better prepare them for finding healthy relationships and in order to maintain a healthy relationship with their current partner, communication and compromise is crucial

Mathematical modeling of transient state transdermal drug delivery

In this work, a two-pathway mathematical model for transdermal drug delivery with iontophoresis is presented. The partial differential equations are described and then solved. An alternative, two-pathway, three-layer model is also presented, and the implications of the coefficients within the equation are discussed. Using Franz cell iontophoretic delivery data from three drug substances (amitriptyline HCl, clomipramine HCl, and nortriptyline HCl), the two-pathway model is regressed to determine the diffusion coefficient and the concentration within the skin at the drug reservoir interface. ANOVA analysis indicates a correlation between iontophoretic current and concentration of drug within the stratum corneum

Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators

CRISPR-mediated gene activation (CRISPRa) can be used to target endogenous genes for activation. By targeting pluripotency-associated reprogramming factors, human fibroblasts can be reprogrammed into induced pluripotent stem cells (iPSCs). Here, we describe a method for the derivation of iPSCs from human fibroblasts using episomal plasmids encoding CRISPRa components. This chapter also provides procedure to assemble guide RNA cassettes and generation of multiplexed guide plasmids for readers who want to design their own guide RNAs.Peer reviewe