Enrichment of a new species of sulfate-reducing bacteria from the Loki´s Castle hydrothermal vent field

Deep-sea hydrothermal vent ecosystems, such as the Barite Field of Loki´s Castle Vent Field (LCVF), host unique microbial communities that drive essential biogeochemical processes, including anaerobic sulfate reduction. This study aimed to enrich and characterize sulfate-reducing bacteria (SRB) from hydrothermal sediments collected at LCVF. Using anaerobic cultivation techniques and metagenomic data to guide substrate selection, 27 enrichment cultures were supplemented with substrates: sodium DL-lactate, sodium 4-hydroxybenzoate, methylamine hydrochloride and trimethylamine hydrochloride in addition to control cultures. Among the tested substrates, only cultures amended with 10 mM sodium DL-lactate stimulated SRB activity, shown by increased sulfide (H2S) concentration measured over time. Repeated dilution series of sodium DL-lactate enrichment culture 904 led to sediment free cultures, dominated by ~5 m rod-shaped cells, consistently associated with high sulfide concentrations. Nanopore long-read sequencing and taxonomic profiling revealed a strong community shift from a diverse environmental inoculum to a low-diversity community dominated by Desulfohalotomaculaceae, a sulfate-reducing bacterial family with limited representatives. Relative abundance of this taxa increased to over 40% in 10 mM lactate enriched cultures and decreased to 1.93% by reducing lactate concentration to 1 mM. This indicated substrate selectivity and potential metabolic specialization for lactate utilization for Desulfohalotomaculaceae. Reduced lactate concentration stimulated increase in fermentative and heterotrophic lineages such as Caminicellaceae. The successful enrichment of this group highlights the value of substrate guided cultivation and support future effort towards isolating and characterizing novel SRB lineages such from extreme environments such as Desulfohalotomaculaceae. Still, in order to fully characterize this species and uncover potential novelty, polyphonic analysis combining genomic, phenotype and phylogenic analyses are necessary.Masteroppgave i biologiBIO399MAMN-BIOMAMN-HAVS

A "Handy" Identification of Artists in the Chocholá Style Ceramic Corpus

Latin American Studie

Bacteria in relation to every day life.

It is to be noticed that if there be any marshy places, certain animals breed there, which are invisible to the eye and yet getting into the system through the mouth and nostrils cause serious disorders. This quaint observation is taken from a manuscript written over two thousand years ago, showing that bacteria even if not seen, were living and active. Latin writers of about the same time recorded a relation between insects and malaria which has but lately been proved and explained. The infectious character of leprosy has long been recognized, since the ancient Hebrews caused the isolation of the afflicted. The laws of Moses point to some knowledge of the nature of infections: This is the law, when a man dieth in a tent; all that come into the tent, and all that is in the tent shall be unclean for several days. And every open vessel which has no covering upon it shall be unclean

Whitman and Van Gogh: Starry Nights and Other Similarities

Explores the similarities between Whitman\u27s poetry and Van Gogh\u27s painting "Starry Night," with a particular emphasis on their similar use of "star imagery.

Perceptual compression of magnitude-detected synthetic aperture radar imagery

A perceptually-based approach for compressing synthetic aperture radar (SAR) imagery is presented. Key components of the approach are a multiresolution wavelet transform, a bit allocation mask based on an empirical human visual system (HVS) model, and hybrid scalar/vector quantization. Specifically, wavelet shrinkage techniques are used to segregate wavelet transform coefficients into three components: local means, edges, and texture. Each of these three components is then quantized separately according to a perceptually-based bit allocation scheme. Wavelet coefficients associated with local means and edges are quantized using high-rate scalar quantization while texture information is quantized using low-rate vector quantization. The impact of the perceptually-based multiresolution compression algorithm on visual image quality, impulse response, and texture properties is assessed for fine-resolution magnitude-detected SAR imagery; excellent image quality is found at bit rates at or above 1 bpp along with graceful performance degradation at rates below 1 bpp

Visual Master Plan, Carver, Minnesota.

Prepared for the City of Carver with financial assistance from the Phelps Fund Committee, the Center for Community Studies (a program of CURA), and the City of Carver

Parametric analysis of dynamic postural responses

A detailed theoretical understanding of postural control mechanisms must be preceded by careful quantification of both the deterministic and stochastic aspects of postural behavior of normal and abnormal subjects under various dynamic conditions. Toward this end, concise parametric transfer function plus noise models were derived for both shoulder and waist position data obtained by applying a linear anteriorposterior bandlimited pseudorandom disturbance to the base of support of human subjects. Model orders as well as model parameters were determined empirically. One advantage of this modeling procedure is the conciseness of the postural models, permitting easy statistical analysis of the data obtained under different dynamic conditions from many subjects. Model features, including pole and zero locations, from 6 normal subjects each tested on 5 consecutive days under 3 input amplitudes and eyes open and closed conditions are presented. The resulting transfer function models consist of only 1 or 2 poles near the integration position on the Z plane unit circle and 0 to 2 zeros. Locations of the poles indicate that the eyes closed responses are more oscillatory, less damped, and with higher gains than the eyes open responses. These transfer functions are similar to nonparametric ones of other authors. The noise model orders are also small. Their spectra are those of low pass systems. Also, the quantity and frequency range of the postural noise is positively related to the amplitude of platform motion as well as related to the presence or absence of vision.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47433/1/422_2004_Article_BF00346137.pd

User manual for IDENT, a parametric and nonparametric linear systems identification package

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/24919/1/0000346.pd