Laminaria japonica Extract Enhances Intestinal Barrier Function by Altering Inflammatory Response and Tight Junction-Related Protein in Lipopolysaccharide-Stimulated Caco-2 Cells.

In the normal physiological state, intestinal epithelial cells act as a defensive frontline of host mucosal immunity to tolerate constant exposure to external stimuli. In this study, we investigated the potential anti-inflammatory and gut permeability protective effects of Laminaria japonica (LJ) water extract (LJE) and three types of fermented Laminaria japonica water extracts (LJE-F1, LJE-F2, and LJE-F3) in lipopolysaccharide (LPS)-stimulated Caco-2, human intestinal epithelial cells. All four extracts significantly decreased the production of nitric oxide and interleukin-6 induced by LPS stimulus. In addition, LJE and the three types of LJE-Fs also inhibited LPS-induced loss of monolayer permeability, as assessed by changes in transepithelial electrical resistance. All four LJ extracts significantly prevented the inhibition of the protein levels of occludin, whereas LJE, LJE-F1, and LJE-F3 significantly attenuated the reduction in phosphorylation of adenosine monophosphate-activated protein kinase compared with the LPS-treated group in Caco-2 cells. In conclusion, LJE and its fermented water extracts appear to have potential gut health-promoting effects by reducing inflammation and partially regulating the tight junction-related proteins in human intestinal epithelial cells. Thus, additional studies are warranted to evaluate Laminaria japonica as a therapeutic agent for inflammatory bowel diseases

Hydrogen-Bonding Network Anchors the Cyclic Form of Sugar Arylhydrazones

The “classical” challenge, raised by Emil Fischer as to why one monosaccharide arylhydrazone adopts a cyclic structure but another an acyclic structure, is answered here. The present comprehensive analysis of hexose and hexosamine arylhydrazones, based on 2D NMR spectroscopy and theoretical modeling, has established that the chain of hydrogen bonds needed for conformational selection can only be completed for D-glucosamine derivatives. Thus, D -glucosamine 4-nitrophenyl-hydrazone exclusively adopts its cyclic form, but any configurational changes imply the formation of acyclic structures. In conclusion, three criteria dominate structure selection, namely 1) an amino function at the C-2 position, 2) the “all-equatorial” substitution mode of the pyranoid ring, and 3) an electron- withdrawing group on the arylhydrazone are all needed to get the cyclic form only

Reducing Nurses\u27 Communication Anxiety During End of Life Care

End of life (EOL) conversations are meaningful discussions pertaining to the patient’s disease trajectory, symptom management, and the patient’s goals at end of life. End of life care discussions have been associated with many benefits for the patient including: decreased healthcare costs, less aggressive treatments at EOL, and a good death. These sensitive discussions must occur over the course of several interactions or spontaneously in the moment in order to support the patient during delicate moments. This makes the nurse the prime candidate to help facilitate and harbor these discussions. However, barriers to communication exist, leading to the omission of EOL conversations, which results in inadequate patient care. Research recommends that nurses who are provided with training and adequate support are able to initiate and facilitate in EOL conversations with patients, leading to positive patient outcomes. The purpose of the project is to develop and implement an educational program about EOL discussions for hospice nursing staff at an inpatient hospice unit with the primary goal of enhancing nurses’ knowledge and understanding of EOL communication, leading to nurse empowerment and improved comfort to engage in EOL discussion. It is proposed that a two-hour, face-to-face educational program with a pre and post intervention survey be implemented in order to decrease nurses’ anxiety when participating in EOL conversations. The implications of the educational program support the use of face-to-face on-the-job training as an effective measure in teaching and enhancing nursing skills, and EOL education should be included in professional development and orientation in areas not traditionally educated in palliative and hospice care

The histone deacetylase inhibitor valproic acid alters growth properties of renal cell carcinoma in vitro and in vivo

Histone deacetylase (HDAC) inhibitors represent a promising class of antineoplastic agents which affect tumour growth, differentiation and invasion. The effects of the HDAC inhibitor valproic acid (VPA) were tested in vitro and in vivo on pre-clinical renal cell carcinoma (RCC) models. Caki-1, KTC-26 or A498 cells were treated with various concentrations of VPA during in vitro cell proliferation 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays and to evaluate cell cycle manipulation. In vivo tumour growth was conducted in subcutaneous xenograft mouse models. The anti-tumoural potential of VPA combined with low-dosed interferon-α (IFN-α) was also investigated. VPA significantly and dose-dependently up-regulated histones H3 and H4 acetylation and caused growth arrest in RCC cells. VPA altered cell cycle regulating proteins, in particular CDK2, cyclin B, cyclin D3, p21 and Rb. In vivo, VPA significantly inhibited the growth of Caki-1 in subcutaneous xenografts, accompanied by a strong accumulation of p21 and bax in tissue specimens of VPA-treated animals. VPA–IFN-α combination markedly enhanced the effects of VPA monotherapy on RCC proliferation in vitro, but did not further enhance the anti-tumoural potential of VPA in vivo. VPA was found to have profound effects on RCC cell growth, lending support to the initiation of clinical testing of VPA for treating advanced RCC

Thermal Degradation of Adsorbed Bottle-Brush Macromolecules: Molecular Dynamics Simulation

The scission kinetics of bottle-brush molecules in solution and on an adhesive substrate is modeled by means of Molecular Dynamics simulation with Langevin thermostat. Our macromolecules comprise a long flexible polymer backbone with $L$ segments, consisting of breakable bonds, along with two side chains of length $N$, tethered to each segment of the backbone. In agreement with recent experiments and theoretical predictions, we find that bond cleavage is significantly enhanced on a strongly attractive substrate even though the chemical nature of the bonds remains thereby unchanged. We find that the mean bond life time decreases upon adsorption by more than an order of magnitude even for brush molecules with comparatively short side chains $N=1 \div 4$. The distribution of scission probability along the bonds of the backbone is found to be rather sensitive regarding the interplay between length and grafting density of side chains. The life time declines with growing contour length $L$ as $\propto L^{-0.17}$, and with side chain length as $\propto N^{-0.53}$. The probability distribution of fragment lengths at different times agrees well with experimental observations. The variation of the mean length $L(t)$ of the fragments with elapsed time confirms the notion of the thermal degradation process as a first order reaction.Comment: 15 pages, 7 figure

Transformative Shift: Exploring Teachers\u27 Dispositions Toward Culturally Relevant Pedagogy in a Community of Practice

In this collaborative, embedded mixed-method study I explored the impact of teacher participation in a community of practice focused on improving attitudes toward and implementation of culturally relevant and responsive pedagogy. Using the Dispositions for Culturally Responsive Pedagogy Scale as a tool to induce critical reflection and dialogue; teachers collaboratively examined their beliefs, practices and perceptions regarding CRP. Through focus group interviews and exit tickets, changes in teacher behaviors and attitudes toward CRP were examined and identified. The goal of this study was to contribute to advancing knowledge on teacher empowerment through transformative learning experiences, CRP implementation, and professional learning in diverse rural settings

Modeling Initial Participation of Diverse Communities in Competitive Swimming

This research note introduces the Initial Participation Model, which theorizes continued participation in a activity or group before individuals make commitment is a function of: enjoyment, feeling of inclusion, and/or involvement opportunities. The specific focus of this research is investigating how deficiency in enjoyment, feeling of inclusion, and involvement opportunities may discourage continuing participation in competitive swimming by underrepresented populations such as African American, Black, Hispanic, Latino, Native American, Pacific Islander and low-socioeconomic communities. Details explain how initial participation differs from other sport stages by emphasizing participation; relating to program instead of sport; and resetting each time an individual joins a new activity or group. Two examples are offered illustrating how the model may be used for identifying points of intervention that stimulate continued initial participation. Also included are specific factors constructing the model and future testing plans for validation