Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis

AKT3 gene is a constituent of the serine/threonine protein kinase family and plays a crucial role in synthesis of milk fats and cholesterol by regulating activity of the sterol regulatory element binding protein (SREBP). AKT3 is highly conserved in mammals and its expression levels during the lactation periods of cattle are markedly increased. AKT3 is highly expressed in the intestine followed by mammary gland and it is also expressed in immune cells. It is involved in the TLR pathways as effectively as proinflammatory cytokines. The aims of this study were to investigate the sequences differences between buffalo and cow. Our results showed that there were substantial differences between buffalo and cow in some exons and noteworthy differences of the gene size in different regions. We also identified the important consensus sequence motifs, variation in 2000 upstream of ATG, substantial difference in the “3′UTR” region, and miRNA association in the buffalo sequences compared with the cow. In addition, genetic analyses, such as gene structure, phylogenetic tree, position of different motifs, and functional domains, were performed to establish their correlation with other species. This may indicate that a buffalo breed has potential resistance to disease, environment changes, and airborne microorganisms and some good production and reproductive traits

Effect of mass media intervention on HIV and AIDS related stigma and discrimination in Ethiopia.

This study was conducted to examine the effect of the Betegna radio (diary-show) programme in mitigating the tide of HIV related stigma and discrimination which are considered a prominent factor that worsens the impact of the epidemic in Ethiopia. The study employed qualitative Individual In-depth Interviews and Focus Group Discussions to generate relevant qualitative data whose analysis revealed that the programme played a significant role in empowering many members of the programme’s target audience with the necessary coping mechanisms against stigma and discriminations associated with HIV and AIDS. However, its effect was rather lower than expected because the programme was broadcast at an awkward time for some members of its target audience and it lacked the entertainment aspect.Key words: Behaviour Change, Denial, Discrimination, HIV and AIDS, Media, Stigma

Risk factors of visceral leishmaniasis in East Africa: a case-control study in Pokot territory of Kenya and Uganda

BACKGROUND: In East Africa, visceral leishmaniasis (VL) is endemic in parts of Sudan, Ethiopia, Somalia, Kenya and Uganda. It is caused by Leishmania donovani and transmitted by the sandfly vector Phlebotomus martini. In the Pokot focus, reaching from western Kenya into eastern Uganda, formulation of a prevention strategy has been hindered by the lack of knowledge on VL risk factors as well as by lack of support from health sector donors. The present study was conducted to establish the necessary evidence-base and to stimulate interest in supporting the control of this neglected tropical disease in Uganda and Kenya. METHODS: A case-control study was carried out from June to December 2006. Cases were recruited at Amudat hospital, Nakapiripirit district, Uganda, after clinical and parasitological confirmation of symptomatic VL infection. Controls were individuals that tested negative using a rK39 antigen-based dipstick, which were recruited at random from the same communities as the cases. Data were analysed using conditional logistic regression. RESULTS: Ninety-three cases and 226 controls were recruited into the study. Multivariate analysis identified low socio-economic status and treating livestock with insecticide as risk factors for VL. Sleeping near animals, owning a mosquito net and knowing about VL symptoms were associated with a reduced risk of VL. CONCLUSIONS: VL affects the poorest of the poor of the Pokot tribe. Distribution of insecticide-treated mosquito nets combined with dissemination of culturally appropriate behaviour-change education is likely to be an effective prevention strategy

Bodyweight Change and CarcassYield Performance of Somali Goats Fed with Groundnut Pod Hulls and a Mixture of Wheat Branand Mustard Seed Cake

The study was conducted at Kombolcha ATVET College, Eastern Ethiopia using 25 intact male yearling Somali goats weighing 18.4±1.5kg at the start of the experiment. The objectives of the study was to examine the effects of urea treated groundnut pod hulls and supplementation of wheat bran and mustard seed cake mixture at a ratio of 3:1, respectively at different levels on body weight change and carcass yield performance of Somali goats. A completely randomized block design was employed and the experimental animals were grouped in five blocks of five goats each based on their initial BW. The goats within a block were randomly assigned to one of the five diets. Treatment feeds consisted feeding of sole untreated groundnut pod hulls (UGNPH) (T1) adlibtum, sole urea treated (UTGNPH) (T2) adlibtum, and supplementation of the UGNPH with the concentrate mixture at graded levels of125g (T3), 250g (T4) and 375g DM/head/day (T5). Daily DM intake of UGNPH was higher (P<0.001) in T1 (407.1 g/head/day) and T3 (388.7g/head/day) compared to T4 (331.5 g/head day) and T5 (272.1 g/head/day), whereas that of UTGNPH was higher (P<0.001) for T2 (471.1 g/day/head) compared to all treatments. Total DM intake was higher (P<0.001) in supplemented groups (T3, T4, and T5) compared to non-supplemented groups (T1and T2). Goats fed sole UGNPH (T1) and UTGNH (T2) lost BW at a rate of 31 and 6 g per day, respectively, whereas supplemented goats gained BW at a rate of 18.9(T3), 40.7(T4), and 53.3g(T5)per day. Goats supplemented with concentrate had higher (P<0.001) weight of hot carcass and empty bodyweight (BW). Better dressing percentage was obtained (P<0.01) in supplemented and UTGNPH group on empty and slaughter BW basis. The result of this experiment demonstrated that urea treatment increased intake of groundnut pod hulls and decreased body weight loss as compared to the UGNPH. We recommend supplementation with 375g DM/day/head of the concentrate mixture to UGNPH based diet for small scale goat fattening programs, since neither UGNPH nor UTGNPH provided sufficient nutrients for growing goats

Sharp Injury and Exposure to Blood and Body Fluids among Health Care Workers in Health Care Centers of Eastern Ethiopia

Background: Health care workers are facing certain occupational hazards because of sharp injury and exposure to human blood and body fluids as a result of handling wastes. Though much attention is paid for the protection of these workers, the number of exposures and injuries do not show a sign of decline from time to time. Objective: To examine the occurrence of sharp injury and exposure to blood and body fluids in health care workers in health care centers in Ethiopia. Methods: In a case-control study, a randomly selected sample of 65 health facilities with 391 cases and 429 controls were studied. Data were collected through a self-administered questionnaire. Detailed analysis of exposure among the health care workers was done by logistic regression analysis with generalized estimating equations model to control correlation effects of responses within the cluster of health facilities. Results: The number of health care workers who got sharp injury was 217 (26.5%). 296 (36.1%) had exposure to blood and body fluids. Working at Harari region (adjusted OR 0.44, 95% CI 0.26 to 0.75) and East Hararghea (adjusted OR 0.61, 95% CI 0.40 to 0.94), being male (adjusted OR 0.56, 95% CI 0.44 to 0.91), and a being nurse (adjusted OR 0.188, 95% CI 0.06 to 0.63) were independent risk factors of the exposure. Conclusion: Regardless of the anticipated low self-reporting for exposure status, the number of health care workers reported having sharp injury and exposure to blood and body fluids was high. Such high exposures indicate that health care workers are at high risk of acquiring blood-borne viral infections such as hepatitis B, hepatitis C, and HIV

Determinants of mortality among under-five children admitted with severe acute malnutrition in Addis Ababa, Ethiopia.

BACKGROUND: Management of severe acute malnutrition (SAM) has been a program priority in Ethiopia, but it remains the leading cause of mortality in under-five children. Hence, this study aimed to identify the incidence density rate of mortality and determinants among under-five children with severe acute malnutrition in St. Paul's Hospital Millennium Medical College, 2012 to 2019. METHODS: A retrospective cohort study was conducted and data were collected using a structured checklist from 673 charts, of which 610 charts were included in the final analysis. The Kaplan-Meier survival curve with Log-rank test was used to estimate the survival time. Bi-variable and multi-variable Cox proportional hazard regression models were fitted to identify determinants of death. Schoenfeld residuals test was used to check a proportional hazard assumption. Goodness of fit of the final model was checked using Nelson Aalen cumulative hazard function against Cox-Snell residual. RESULTS: In this study, 61 (10%) children died making the incidence density rate of death 5.6 (95% CI: 4.4, 7.2) per 1000 child-days. Shock (Adjusted Hazard Ratio) [AHR] =3.2; 95% CI: 1.6, 6.3)), IV fluid infusion (AHR = 5.2; 95% CI: 2.4, 10.4), supplementing F100 (AHR = 0.12; 95%CI: 0.06, 0.23) and zinc (AHR = 0.45; 95% CI: 0.22, 0.93) were determinants of death. CONCLUSION: The overall proportion of deaths was within the range put forth by the Sphere standard and the national SAM management protocol. Shock and IV fluid infusion increased the hazard of death, whereas F100 & zinc were found to decrease the likelihood death. Children with SAM presented with shock should be handled carefully and IV fluids should be given with precautions

Magnitude and Associated Factors of Neural Tube Defects in Ethiopia: A Systematic Review and Meta-Analysis.

Neural tube defects remain a major problem in developing countries, but there are limited comprehensive national reports to date in Ethiopia. Therefore, this study aimed to assess the prevalence of neural tube defects and associated factors in Ethiopia. Electronic databases and other sources were used to retrieve studies. Fifteen out of 862 studies were included in the final analysis. The estimated pooled prevalence of neural tube defects among children in Ethiopia was 63.3 cases per 10 000 children. The pooled prevalence of spinal bifida, anencephaly, and encephalocele was 41.09, 18.90, and 1.07 per 10 000 children, respectively. Previous family history and unplanned pregnancy were risk factors for neural tube defects. Folic acid supplementation during the first trimester of pregnancy was found to be protective. Neural tube defects are widespread in Ethiopia. Hence, fortification of food with folic acid or folic acid supplementation during childbearing age is recommended

Statistical bias correction of regional climate model simulations for climate change projection in the Jemma sub-basin, upper Blue Nile Basin of Ethiopia

This study evaluates bias correction methods and develops future climate scenarios using the output of a better bias correctiontechnique at the Jemma sub-basin. The performance of different bias correction techniques was evaluated using several statisticalmetrics. The bias correction methods performance under climate condition different from the current climate was also evaluatedusing the differential split sample testing (DSST) and reveals that the distribution mapping technique is valid under climatecondition different from the current climate. All bias correction methods were effective in adjusting mean monthly and annualRCM simulations of rainfall and temperature to the observed rainfall and temperature values. However, distribution mappingmethod was better in capturing the 90th percentile of observed rainfall and temperature and wet day probability of observedrainfall than other methods. As a result, we use the future (2021–2100) simulation of RCMs which are bias corrected usingdistribution mapping technique. The output of bias-adjusted RCMs unfolds a decline of rainfall, a persistent increase of temperature and an increase of extremes of rainfall and temperature in the future climate under emission scenarios of RepresentativeConcentration Pathways 4.5, 8.5 and 2.6 (RCP4.5, RCP8.5 and RCP2.6). Thus, climate adaptation strategies that can provideoptimal benefits under different climate scenarios should be developed to reduce the impact of future climate change

Observed changes in extremes of daily rainfall and temperature in Jemma Sub-Basin, Upper Blue Nile Basin, Ethiopia

Climate variability has been a threat to the socio-economic development of Ethiopia. This paper examined the changes in rainfall, minimum, and maximum temperature extremes of Jemma Sub-Basin of the Upper Blue Nile Basin for the period of 1981 to 2014. The nonparametric Mann-Kendall, seasonal Mann-Kendall, and Sen’s slope estimator were used to estimate annual trends. Ten rainfall and 12 temperature indices were used to study changes in rainfall and temperature extremes. The results showed an increasing trend of annual and summer rainfall in more than 78% of the stations and a decreasing trend of spring rainfall in most of the stations. An increase in rainfall extreme events was detected in the majority of the stations. Several rainfall extreme indices showed wetting trends in the sub-basin, whereas limited indices indicated dryness in most of the stations. Annual maximum and minimum temperature and extreme temperature indices showed warming trend in the sub-basin. Presence of extreme rainfall and a warming trend of extreme temperature indices may suggest signs of climate change in the Jemma Sub-Basin. This study, therefore, recommended the need for exploring climate induced risks and implementing appropriate climate change adaptation and mitigation strategies