Transforming High School Teaching and Learning: A District-wide Design

High school improvement is one of the most pressing issues facing American education but little attention has been paid to reform strategies that will improve teaching and learning. Drawing on the expertise of teachers, principals, superintendents, policy makers and researchers, a new paper from the Aspen Institute Program on Education, Transforming High School Teaching and Learning: A District-wide Design by Aspen Senior Fellow Judy Wurtzel, offers both a new framework and concrete suggestions for a new approach to high school improvement across an urban school district. The data on high school student performance and graduation rates make clear that significant increases in student achievement are necessary if all students are to graduate from high school fully prepared for post-secondary education, citizenship, and work. Recent high school reform has focused on organizational aspects of high school, particularly creating a wide variety of smaller schools, smaller learning communities, and alternative learning pathways to meet the needs of young people. However, while smaller schools may create the relationships and conditions that make high quality instruction possible, improved instruction and achievement does not flow directly from them. Given this track record, questions facing the high school reform movement include: -- What will it take to get high school instructional improvement that results in demonstrated increases in student learning? -- What supports do high school teachers need to be successful in improving instruction and from where will they get them? -- What changes affecting the professional role, knowledge, and skills of teachers are needed if reforms are to be successful? Though the ideas represented in the paper are not new -- some school districts and states have implemented some of elements described -- what is useful is the attempt to lay out a fairly comprehensive picture of high school instructional reform and to push the conversation about high school instructional improvement into some new territory. First, the paper builds on work done in many urban districts at the K- 8 level to create systems of "managed instruction," that is, deliberate efforts to align common curriculum and instructional materials, formative and benchmark assessments, extensive professional development, and instructional leaders who support a shared set of instructional practices. Second, the paper suggests how these approaches can be developed and implemented in ways that are both consistent with and reinforcing of a robust vision of teacher professionalism. Third, the paper recognizes the urgency of attracting and retaining a teacher workforce that embraces this new job description for high school teachers and can effect improvements in student learning. Finally, it is useful to note that this paper focuses primarily on the district role in improving high school instruction. This is because it seems increasingly clear that school districts are a key unit for instructional improvement. However, much of what is described here could be initiated or supported by states, by consortia of districts, or by networks of managed schools within or across districts

Planarian Epidermal Stem Cells Respond to Positional Cues to Promote Cell-Type Diversity

Successful regeneration requires that progenitors of different lineages form the appropriate missing cell types. However, simply generating lineages is not enough. Cells produced by a particular lineage often have distinct functions depending on their position within the organism. How this occurs in regeneration is largely unexplored. In planarian regeneration, new cells arise from a proliferative cell population (neoblasts). We used the planarian epidermal lineage to study how the location of adult progenitor cells results in their acquisition of distinct functional identities. Single-cell RNA sequencing of epidermal progenitors revealed the emergence of distinct spatial identities as early in the lineage as the epidermal neoblasts, with further pre-patterning occurring in their post-mitotic migratory progeny. Establishment of dorsal-ventral epidermal identities and functions, in response to BMP signaling, required neoblasts. Our work identified positional signals that activate regionalized transcriptional programs in the stem cell population and subsequently promote cell-type diversity in the epidermis.National Institutes of Health (U.S.) (Grant R01GM080639

GRK6 regulates the hemostatic response to injury through its rate-limiting effects on GPCR signaling in platelets.

G protein-coupled receptors (GPCRs) mediate the majority of platelet activation in response to agonists. However, questions remain regarding the mechanisms that provide negative feedback toward activated GPCRs to limit platelet activation and thrombus formation. Here we provide the first evidence that GPCR kinase 6 (GRK6) serves this role in platelets, using GRK6-/- mice generated by CRISPR-Cas9 genome editing to examine the consequences of GRK6 knockout on GPCR-dependent signaling. Hemostatic thrombi formed in GRK6-/- mice are larger than in wild-type (WT) controls during the early stages of thrombus formation, with a rapid increase in platelet accumulation at the site of injury. GRK6-/- platelets have increased platelet activation, but in an agonist-selective manner. Responses to PAR4 agonist or adenosine 5\u27-diphosphate stimulation in GRK6-/- platelets are increased compared with WT littermates, whereas the response to thromboxane A2 (TxA2) is normal. Underlying these changes in GRK6-/- platelets is an increase in Ca2+ mobilization, Akt activation, and granule secretion. Furthermore, deletion of GRK6 in human MEG-01 cells causes an increase in Ca2+ response and PAR1 surface expression in response to thrombin. Finally, we show that human platelet activation in response to thrombin causes an increase in binding of GRK6 to PAR1, as well as an increase in the phosphorylation of PAR1. Deletion of GRK6 in MEG-01 cells causes a decrease in PAR1 phosphorylation. Taken together, these data show that GRK6 regulates the hemostatic response to injury through PAR- and P2Y12-mediated effects, helping to limit the rate of platelet activation during thrombus growth and prevent inappropriate platelet activation

The Single-Nucleotide Resolution Transcriptome of Pseudomonas aeruginosa Grown in Body Temperature

One of the hallmarks of opportunistic pathogens is their ability to adjust and respond to a wide range of environmental and host-associated conditions. The human pathogen Pseudomonas aeruginosa has an ability to thrive in a variety of hosts and cause a range of acute and chronic infections in individuals with impaired host defenses or cystic fibrosis. Here we report an in-depth transcriptional profiling of this organism when grown at host-related temperatures. Using RNA-seq of samples from P. aeruginosa grown at 28°C and 37°C we detected genes preferentially expressed at the body temperature of mammalian hosts, suggesting that they play a role during infection. These temperature-induced genes included the type III secretion system (T3SS) genes and effectors, as well as the genes responsible for phenazines biosynthesis. Using genome-wide transcription start site (TSS) mapping by RNA-seq we were able to accurately define the promoters and cis-acting RNA elements of many genes, and uncovered new genes and previously unrecognized non-coding RNAs directly controlled by the LasR quorum sensing regulator. Overall we identified 165 small RNAs and over 380 cis-antisense RNAs, some of which predicted to perform regulatory functions, and found that non-coding RNAs are preferentially localized in pathogenicity islands and horizontally transferred regions. Our work identifies regulatory features of P. aeruginosa genes whose products play a role in environmental adaption during infection and provides a reference transcriptional landscape for this pathogen

Symmetry For Symmetry’s Sake: Why Bose Does Not Require Independent Review of a Trial Court’s First-Amendment-Favorable Findings of Fact

This Note argues that Bose does not support the symmetrical application of independent review of facts by appellate courts in First Amendment cases, regardless of whether the First Amendment claimant won or lost below. While symmetrical procedures and results may be desirable in most parts of the law, symmetry is not required where that symmetry will inhibit a greater constitutional interest. In the independent review context, symmetrical application of Bose results in the reversal of First Amendment wins that would otherwise be upheld under clear error review. This result is clearly antithetical to Bose’s purpose of enhancing First Amendment protections

Becoming an Artist: Embodying Emergent Art Making Practices

Absract: Written in layers, this creative essay invites the reader to consider the relationship between one’s own becoming and emergent practices in the teaching and making of art. Weaving between a discussion on the theoretical concept of becoming and emergence, along with a personal narrative presented with images, the author tries to demonstrate what emergence might feel like in the body while creating alongside-and-with her own child. From points of disruption to points of harmonizing with material and material bodies, this essay examines emergence through the lens of an embodied relationality and offers up potential ways to experience such practices

Unfitness to Plead. Volume 1: Report.

This has been produced along with Volume 2: Draft Legislation as a combined document Presented to Parliament pursuant to section 3(2) of the Law Commissions Act 1965 Ordered by the House of Commons to be printed on 12 January 201

Traces of Communal Touch: Matrixial Encounters With Pre-service Art Educators Across Two States

The matrixial sphere, as put forth by artist and psychoanalyst Bracha L. Ettinger, is a shared and transsubjective space that is beneath the conscious, under the touchable and perceptible; it is a space where we encounter ourselves and one another in shared trauma, healing, art making, and the lived experience overall. This co-authored essay explores how a shared matrixial space and the matrixial gaze, which is not always visual, have been experienced through the square boxes of ZOOM as part of a collaborative, long-distance art exchange project. The project, focused on Spontaneous Creation-Making (SCM) sessions, as outlined in Barbara Bickel and R. Michael Fishers recent book Art-care Practices for Restoring the Communal: Education, Co-inquiry, and Healing. These exchanges took place over the span of one semester and consisted of two higher education pre-service art education professors in different North American states. This essay looks generatively at moments where the authors and their students found themselves in a creative space that was not entirely internal or external, not entirely here nor there in terms of location, product, and predictability of the process. Using the lens of the Ettingerian matrixial, the authors question, examine, and pursue an investigation of moments where they, alongside their students, encountered the matrixial space in and through the cross-country SCM project. Applications of this unique art exchange are suggested for other art educators and those pursuing art-care practices in general

Transcriptional Response of Mucoid Pseudomonas aeruginosa to Human Respiratory Mucus

Adaptation of bacterial pathogens to a host can lead to the selection and accumulation of specific mutations in their genomes with profound effects on the overall physiology and virulence of the organisms. The opportunistic pathogen Pseudomonas aeruginosa is capable of colonizing the respiratory tract of individuals with cystic fibrosis (CF), where it undergoes evolution to optimize survival as a persistent chronic human colonizer. The transcriptome of a host-adapted, alginate-overproducing isolate from a CF patient was determined following growth of the bacteria in the presence of human respiratory mucus. This stable mucoid strain responded to a number of regulatory inputs from the mucus, resulting in an unexpected repression of alginate production. Mucus in the medium also induced the production of catalases and additional peroxide-detoxifying enzymes and caused reorganization of pathways of energy generation. A specific antibacterial type VI secretion system was also induced in mucus-grown cells. Finally, a group of small regulatory RNAs was identified and a fraction of these were mucus regulated. This report provides a snapshot of responses in a pathogen adapted to a human host through assimilation of regulatory signals from tissues, optimizing its long-term survival potential