Recent developments on fractal-based approaches to nanofluids and nanoparticle aggregation

This project was supported by the National Natural Science Foundation of China (Nos. 41572116, 51576114, ​41630317), the Fundamental Research Funds for the Central Universities, China University of Geosciences (Wuhan) (No. CUG160602) and the Natural Science Foundation of Fujian Province of China (No. 2016J01254). The authors of the figures that used in presented review are also highly appreciated.Peer reviewedPostprin

Fractal analysis of the effect of particle aggregation distribution on thermal conductivity of nanofluids

This project was supported by the National Natural Science Foundation of China (No. 41572116), the Fundamental Research Funds for the Central Universities, China University of Geosciences, Wuhan) (No. CUG160602).Peer reviewedPostprin

Inversion and Interpretation of Magnetic Anomaly in the Presence of Significant Remanence and Self-Demagnetization Based on Magnetic Amplitude

Remanent magnetization and self-demagnetization effects of high-susceptibility body distort the intensity and direction of internal magnetization and hence complicate the inversion and interpretation of magnetic anomaly. The magnitude magnetic anomaly, which is weakly sensitive to the magnetization direction, provides an indirect way to investigate these complex anomalies. We study the sensitivity characteristics of 2D magnitude magnetic anomaly to magnetization direction and source shapes, implement the magnetization intensity inversion, and further estimate the magnetization direction by inverting for the total field data. The magnetic amplitude inversion is tested by the use of synthetic data, which are caused by prism models with strong remanent magnetization and high susceptibility. It is also applied to the field data of an iron-ore deposit in South Australia. The primary advantage of magnitude anomaly inversion is that the magnetization directions are not assumed to parallel the geomagnetic field. The magnetization intensity inversion and magnetization direction estimation make full use of the amplitude and phase information of magnetic anomalies. Magnetic amplitude inversion including other amplitude quantities such as normalized source strength and analytic signal offers an effective approach to investigate and interpret the magnetic anomalies affected by complicated remanence and self-demagnetization

Enhanced Automated Quality Assessment Network for Interactive Building Segmentation in High-Resolution Remote Sensing Imagery

In this research, we introduce the enhanced automated quality assessment network (IBS-AQSNet), an innovative solution for assessing the quality of interactive building segmentation within high-resolution remote sensing imagery. This is a new challenge in segmentation quality assessment, and our proposed IBS-AQSNet allievate this by identifying missed and mistaken segment areas. First of all, to acquire robust image features, our method combines a robust, pre-trained backbone with a lightweight counterpart for comprehensive feature extraction from imagery and segmentation results. These features are then fused through a simple combination of concatenation, convolution layers, and residual connections. Additionally, ISR-AQSNet incorporates a multi-scale differential quality assessment decoder, proficient in pinpointing areas where segmentation result is either missed or mistaken. Experiments on a newly-built EVLab-BGZ dataset, which includes over 39,198 buildings, demonstrate the superiority of the proposed method in automating segmentation quality assessment, thereby setting a new benchmark in the field.Comment: The manuscript is submitted to IEEE International Geoscience and Remote Sensing Symposium(IGARSS2024

Construction of a camelid VHH yeast two-hybrid library and the selection of VHH against haemagglutinin-neuraminidase protein of the Newcastle disease virus

Humoral immune response after immunization. Sera from IIama was collected, two-fold diluted and tested by HI using LaSota as antigen. Figure S1 Amplification of VHH through a nested PCR. (A) First round PCR to separate VH from VHH. The upper 900 bp bands represent the VH-CH1-Hinge-CH2 of conventional Abs (lane 1–8). The lower 600 bp bands represent the VHH-Hinge-CH2 of HCAbs (lane 1–8). (B) VHH amplified through nested PCR using 600 bp fragment recovered from first round PCR as template (lane 1–4). M in A and B was the DL2000 DNA marker. C in A and B represent the negative control. Figure S2 PCR identification of inserted VHH. 47 clones were randomly picked to determine the library functional diversity by PCR using universal primers T7 and 3’AD (Table 1). Meanwhile, Sterile water was used as negative controls. 45 clones have amplified the 500 bp VHH fragments (lane 1–47), while negative templates control haven’t amplified any bands (lane C). M indicated the DL2000 DNA marker. Figure S3 Detection of library capacity and library titer. (A) 10-3 dilution plating of the transformed cells calculated a library capacity of 1.25 × 107 independent clones. (B) 10-5 dilution plating of the cultured library indicated a library titer of 3.45 × 108 cfu/mL. Figure S4 Deduced amino acid aligment of 10 random picked VHH. Deduced amino acid sequences were analyzed according to the Kabat numbering. Differences in the sequences are pinked, and the dash represent the missing sequences. Two hallmark Cys residues are labeled by the thick-line boxes. The four conservative hallmark residues of VHH in FR2 are labeled by the dotted line boxes. Figure S5 pGBKT7-HN bait plasmid construction. (A) PCR was carried out to amplify a truncate HN gene (without transmembrane region) from La Sota strain. M, 5000 DNA marker. 1, Truncate HN. C, Negative control. (B) A truncate HN was cloned into pGBKT7 through BamH I and Sal I. M, 5000 DNA marker. 1, Double restriction enzyme digestion of pGBKT7-HN. Figure S6 pHSIE-VHH plasmid construction. (A) 7 positive VHH fragment were amplified from recovered positive clones containing pGADT7-VHH by PCR. M, 5000 DNA marker. 1–7, VHH 1–7. C, Negative control. (B) Double restriction enzyme digestion of pHSIE-VHHs. M, 5000 DNA marker. 1–7, pHSIE-VHH 1–7. Figure S7 Western blot analysis of bait protein expression. 2 mL of Y2HGold(pGBKT7-HN) culture liquid was extracted using yeast protein extraction reagent (Takara). c-Myc tag monoclonal antibody (1:4000 dilution) was used as first antibody and HRP-labeled goat anti-mouse antibody (1:5000) was used as second antibody. The immunoreactive was visualized with cECL Plus Western blotting detection reagent (CWBIO). (DOC 1129 kb