Serum N-Terminal Type III Procollagen Propeptide: An Indicator of Growth Hormone Excess and Response to Treatment in Feline Hypersomatotropism

BACKGROUND: N‐terminal type III procollagen propeptide (PIIINP) is a biomarker of soft tissue proliferation. Hypersomatotropism (HS) is associated with soft tissue proliferation. HYPOTHESIS: Serum PIIINP is increased in cats with HS and decreases with effective treatment, and may be an additional tool in the diagnosis and treatment of feline HS. ANIMALS: Cats with uncomplicated diabetes mellitus (DM; n = 30) and with HS‐induced DM (HSDM; n = 30). Pre‐ and posttreatment samples were available from 5 cats undergoing radiotherapy (RT) and 16 cats undergoing hypophysectomy (HPX). METHODS: Retrospective and prospective cross‐sectional study. Analytical performance of a serum PIIINP ELISA was assessed and validated for use in cats. PIIINP and insulin‐like growth factor 1 (IGF‐1) radioimmunoassays (RIA) were performed pre‐ and post‐treatment in cats with DM and HSDM. PIIINP and IGF‐1 were compared between cats treated by RT and HPX. RESULTS: Serum PIIINP concentrations were significantly higher (P < .001) in HSDM cats (median, 19.6 ng/mL; range, 1.7–27.9) compared to DM cats (median, 5.0 ng/mL; range, 2.1–10.4). A cut‐off of 10.5 ng/mL allowed differentiation between DM and HSDM cats with 87% sensitivity and 100% specificity (area under the curve [AUC], 0.91; 95% confidence interval [CI], 0.82‐1). After RT, PIIINP increased significantly (P = .043) with no significant change in IGF‐1 concentrations. After HPX, serum PIIINP (P = .034) and IGF‐1 concentrations (P < .001) decreased significantly. CONCLUSION AND CLINICAL IMPORTANCE: PIIINP concentrations are increased in cats with untreated HSDM compared to those with DM, demonstrating the effect of excess GH on soft tissue. PIIINP concentrations decreased after HPX in most HSDM cats

Feline hypersomatotropism and acromegaly tumorigenesis: a potential role for the AIP gene

Acromegaly in humans is usually sporadic, however up to 20% of familial isolated pituitary adenomas are caused by germline sequence variants of the aryl-hydrocarbon-receptor interacting protein (AIP) gene. Feline acromegaly has similarities to human acromegalic families with AIP mutations. The aim of this study was to sequence the feline AIP gene, identify sequence variants and compare the AIP gene sequence between feline acromegalic and control cats, and in acromegalic siblings. The feline AIP gene was amplified through PCR using whole blood genomic DNA from 10 acromegalic and 10 control cats, and 3 sibling pairs affected by acromegaly. PCR products were sequenced and compared with the published predicted feline AIP gene. A single nonsynonymous SNP was identified in exon 1 (AIP:c.9T > G) of two acromegalic cats and none of the control cats, as well as both members of one sibling pair. The region of this SNP is considered essential for the interaction of the AIP protein with its receptor. This sequence variant has not previously been reported in humans. Two additional synonymous sequence variants were identified (AIP:c.481C > T and AIP:c.826C > T). This is the first molecular study to investigate a potential genetic cause of feline acromegaly and identified a nonsynonymous AIP single nucleotide polymorphism in 20% of the acromegalic cat population evaluated, as well as in one of the sibling pairs evaluated

Effect of stellar flares on the upper atmospheres of HD 189733b and HD 209458b

Stellar flares are a frequent occurrence on young low-mass stars around which many detected exoplanets orbit. Flares are energetic, impulsive events, and their impact on exoplanetary atmospheres needs to be taken into account when interpreting transit observations. We have developed a model to describe the upper atmosphere of Extrasolar Giant Planets (EGPs) orbiting flaring stars. The model simulates thermal escape from the upper atmospheres of close-in EGPs. Ionisation by solar radiation and electron impact is included and photochemical and diffusive transport processes are simulated. This model is used to study the effect of stellar flares from the solar-like G star HD209458 and the young K star HD189733 on their respective planets. A hypothetical HD209458b-like planet orbiting the active M star AU Mic is also simulated. We find that the neutral upper atmosphere of EGPs is not significantly affected by typical flares. Therefore, stellar flares alone would not cause large enough changes in planetary mass loss to explain the variations in HD189733b transit depth seen in previous studies, although we show that it may be possible that an extreme stellar proton event could result in the required mass loss. Our simulations do however reveal an enhancement in electron number density in the ionosphere of these planets, the peak of which is located in the layer where stellar X-rays are absorbed. Electron densities are found to reach 2.2 to 3.5 times pre-flare levels and enhanced electron densities last from about 3 to 10 hours after the onset of the flare. The strength of the flare and the width of its spectral energy distribution affect the range of altitudes that see enhancements in ionisation. A large broadband continuum component in the XUV portion of the flaring spectrum in very young flare stars, such as AU Mic, results in a broad range of altitudes affected in planets orbiting this star.Comment: accepted for publication in A&

Prospective evaluation of a protocol for transitioning porcine lente insulintreated diabetic cats to human recombinant protamine zinc insulin

Objectives The objective was to evaluate a nadir-led protocol for transitioning porcine lente insulin suspension (PLIS)-treated diabetic cats onto human recombinant protamine zinc insulin (PZIR). Methods Recently diagnosed (<5 months) diabetic cats, treated with PLIS q12h for 6 weeks, were recruited. Fructosamine, 24 h blood glucose curve (BGC), quality of life assessment (DIAQoL-pet score) and Diabetic Clinical Score (DCS) were assessed at enrolment (PLIS-treated) and 2, 4 and 12 weeks after transitioning to PZIR (starting dose 0.2-0.7 U/kg q12h). Short duration of insulin action was defined as <9 h. Linear mixed effects modelling assessed for change in fructosamine, mean blood glucose (MBG) during BGCs, DIAQoL-pet score, DCS and q12h insulin dose. McNemar's tests compared the proportion of cats with hypoglycaemia at week 0 (PLIS-treated) and week 4 (PZIR-treated). Results Twenty-two cats were recruited. Median PLIS dose at enrolment was 0.5 U/kg (interquartile range 0.3-0.7 U/kg) q12h, equalling median PZIR starting dose (0.5 U/kg; interquartile range 0.3-0.7 U/kg q12h). Transitioning was followed by significant decreases in fructosamine (P = 0.00007), insulin dose (P = 0.02), DCS (P = 8.1 x 10(-8)) and DIAQoL-pet score (P = 0.003), indicating improved quality of life. MBG did not alter significantly (P = 0.1). Five cats (22.7%) achieved remission. Hypoglycaemia was recorded in 30/190 12 h BGCs (15.8%) and five cats experienced clinical hypoglycaemia. The proportion of cats with hypoglycaemia did not differ between PLIS (week 0) and PZIR (week 4) (P = 1.0). Duration of action was analysed in 19 cats. Six cats (31.6%) showed short duration of action on PLIS, compared with two cats (10.5%) after 4 weeks on PZIR. All six cats with short PLIS duration showed duration of 9 h on PZIR. Conclusions and relevance Used alongside a low-carbohydrate diet, transitioning to PZIR was associated with significantly improved clinical signs and quality of life, with some cats achieving remission. Transition to PZIR should be considered for cats with short duration of action on PLIS

XMM-Newton observations of the sigma Ori cluster. II. Spatial and spectral analysis of the full EPIC field

We present the results of an XMM-Newton observation of the young (~2-4 Myr) cluster around the hot star sigma Orionis. In a previous paper we presented the analysis of the RGS spectrum of the central hot star; here we discuss the results of the analysis of the full EPIC field. We have detected 175 X-ray sources, 88 of which have been identified with cluster members, including very low-mass stars down to the substellar limit. We detected eleven new possible candidate members from the 2MASS catalogue. We find that late-type stars have a median log L_X/L_bol ~ -3.3, i.e. very close to the saturation limit. We detected significant variability in ~40% of late-type members or candidates, including 10 flaring sources; rotational modulation is detected in one K-type star and possibly in another 3 or 4 stars. Spectral analysis of the brightest sources shows typical quiescent temperatures in the range T_1 ~ 0.3-0.8 keV and T_2 ~ 1-3 keV, with subsolar abundances Z ~ 0.1-0.3 Z_sun, similar to what is found in other star-forming regions and associations. We find no significant difference in the spectral properties of classical and weak-lined T Tauri stars, although classical T Tauri stars tend to be less X-ray luminous than weak-lined T Tauri stars.Comment: 20 pages, 10 figures, 6 tables. Accepted by A&

FLAMES spectroscopy of low-mass stars in the young clusters sigma Ori and lambda Ori

Aims. We performed a detailed membership selection and studied the accretion properties of low-mass stars in the two apparently very similar young (1-10 Myr) clusters sigma Ori and lambda Ori. Methods. We observed 98 and 49 low-mass (0.2-1.0 M_sun) stars in sigma Ori and lambda Ori respectively, using the multi-object optical spectrograph FLAMES at the VLT, with the high-resolution (R=17,000) HR15N grating (6470-6790 AA). We used radial velocities, Li and Halpha to establish cluster membership and Halpha and other optical emission lines to analyze the accretion properties of members. Results. We identified 65 and 45 members of the sigma Ori and lambda Ori clusters, respectively and discovered 16 new candidate binary systems. We also measured rotational broadening for 20 stars and estimated the mass accretion rates in 25 stars of the sigma Ori cluster, finding values between 10^-11 and 10^-7.7 M_sun yr^-1 and in 4 stars of the lambda Ori cluster, finding values between 10^-11 and 10^-10.1 M_sun yr-1. Comparing our results with the infrared photometry obtained by the Spitzer satellite, we find that the fraction of stars with disks and the fraction of active disks is larger in the sigma Ori cluster (52+-9% and 78+-16%) than in lambda Ori (28+-8% and 40+-20%) Conclusions. The different disk and accretion properties of the two clusters could be due either to the effect of the high-mass stars and the supernova explosion in the lambda Ori cluster or to different ages of the cluster populations. Further observations are required to draw a definitive conclusion.Comment: 14 pages, 9 figures, 9 tables, accepted for publications in A&

Introduced marine macroflora of Lebanon and its distribution on the Levantine coast

The marine macroflora introduced into Lebanon was studied through an analysis of historical data and field studies carried out since 1991. A total of 29 introduced species have been listed: 3 Chromobionta, 13 Rhodobionta, 12 Chlorobionta and 1 Streptobionta. Among them, Caulerpa taxifolia var. distichophylla, Spatoglossum variabile and Womersleyella setacea are recorded for the first time from Lebanon. Among them, Asparagopsis taxiformis, C. taxifolia var. distichophylla, Cladophora patentiramea, Codium arabicum, Codium parvulum, Galaxaura rugosa, Halophila stipulacea, Hypnea cornuta, Laurencia cf. chondrioides, Lophocladia lallemandii, Stypopodium schimperi, Ulva lactuca and Womersleyella setacea are potentially invasive species, and several of them already occupy extensive stretches of the Lebanese coast. The distribution of species on the Levantine coast, their dynamics and the current status of their populations are discussed.The surveys in 2012, 2013 and 2016 were conducted within the framework of the MedMPAnet Project, implemented by UNEP/MAP-RAC/SPA and funded by RAC/SPA, the European Commission (EC), the Spanish Agency for International Cooperation for Development (AECID), and the French Global Environment Facility (FFEM)

Low-resolution spectroscopy and spectral energy distributions of selected sources towards sigma Orionis

Aims: We investigated in detail nine sources in the direction of the young sigma Orionis cluster, which is considered a unique site for studying stellar and substellar formation. The nine sources were selected because of some peculiar properties, such as extremely red infrared colours or too strong Halpha emission for their blue optical colours. Methods: We took high-quality, low-resolution spectroscopy (R ~ 500) of the nine targets with ALFOSC at the Nordic Optical Telescope. We also re-analyzed [24]-band photometry from MIPS/Spitzer and compiled the best photometry available at the ViJHKs passbands and the four IRAC/Spitzer channels for constructing accurate spectral energy distributions covering from 0.55 to 24 mum. Results: The nine targets were classified into: one Herbig Ae/Be star with a scatterer edge-on disc, two G-type stars, one X-ray flaring, early-M, young star with chromospheric Halpha emission, one very low-mass, accreting, young spectroscopic binary, two young objects at the brown dwarf boundary with the characteristics of classical T Tauri stars, and two emission-line galaxies, one undergoing star formation, and another one whose spectral energy distribution is dominated by an active galactic nucleus. Besides, we discover three infrared sources associated to overdensities in a cold cloud in the cluster centre. Conclusions: Low-resolution spectroscopy and spectral energy distributions are a vital tool for measuring the physical properties and the evolution of young stars and candidates in the sigma Orionis cluster.Comment: Accepted for publication in A&

HRC-I/Chandra X-ray observations towards sigma Orionis

Aims: We investigated the X-ray emission from young stars and brown dwarfs in the sigma Orionis cluster (tau~3 Ma, d~385 pc) and its relation to mass, presence of circumstellar discs, and separation to the cluster centre by taking advantage of the superb spatial resolution of the Chandra X-ray Observatory. Methods: We used public HRC-I/Chandra data from a 97.6 ks pointing towards the cluster centre and complemented them with X-ray data from IPC/Einstein, HRI/ROSAT, EPIC/XMM-Newton, and ACIS-S/Chandra together with optical and infrared photometry and spectroscopy from the literature and public catalogues. On our HRC-I/Chandra data, we measured count rates, estimated X-ray fluxes, and searched for short-term variability. We also looked for long-term variability by comparing with previous X-ray observations. Results: Among the 107 detected X-ray sources, there were 70 cluster stars with known signposts of youth, two young brown dwarfs, 12 cluster member candidates, four field dwarfs, and two galaxies with optical-infrared counterpart. The remaining sources had extragalactic nature. Based on a robust Poisson-chi^2 analysis, nine cluster stars displayed flares or rotational modulation during the HRC-I observations, while other eight stars and one brown dwarf showed long-term X-ray flux variations. We constructed a cluster X-ray luminosity function from O9.5 (~18 Msol) to M6.5 (~0.06 Msol). We found: a tendency of early-type stars in multiple systems or with spectroscopic peculiarities to display X-ray emission, that the two detected brown dwarfs and the least-massive star are among the sigma Orionis objects with the highest L_X/L_J ratios, and that a large fraction of known classical T Tauri stars in the cluster are absent in this and other X-ray surveys. We concluded that dozens X-ray sigma Orionis stars and brown dwarfs are still to be detected [abridged].Comment: A&A, in pres