Qualité microbiologique du lait cru ovin collecte dans la steppe centrale de l’Algérie

L’objectif de l’étude consiste à évaluer la qualité microbiologique du lait cru ovin collecté localement, en milieu steppique Algérien dans la région de Djelfa situé à 300 km au sud d’Alger. Au total 51 échantillons de lait ont été prélevés pour des fins analytiques. La contamination moyenne est de 2,3.107 cfu/mL pour la flore totale contre 1,1.105 pour les coliformes totaux et 1,5.104 pour les coliformes fécaux. Pour la flore fongique, les laits sont plus contaminés en levures qu’en moisissures (2,4.105 vs 3,4.103 germes/mL respectivement). Les Streptocoques fécaux sont détectés chez 43,14% des échantillons par contre E.coli n’est décelée que chez 17,65% des laits. Par ailleurs, 28% des prélèvements sont positifs vis-à-vis de Clostridium contre 9,8% pour Staphylococcus aureus. En revanche, tous les laits sont exempts de Salmonella. Pour ce qui est de la recherche des anticorps brucelliques, 13,73% des laits analysés sont positifs au Ring-test. La mauvaise qualité du lait reflète bien les conditions déplorables de production et la non-observance des bonnes pratiques hygiéniques lors de la traiteMots-clés : brebis, lait cru, qualité hygiénique, microorganismes pathogènes, Algérie.Microbiological quality of raw ewe’s milk in Algerian middle steppeThe objective of this study was to assess the microbiological quality of raw milk sheep collected in Algerian steppe. A total of 51 samples of bulk milk were examined. The results obtained show the unsatisfactory quality of milk analyzed. The average contamination is 2,3.107 cfu/ml for the total flora against 1,1.105 for total coliforms and 1,5.104 for fecal coliforms. For the fungal flora, milk is more contamined with yeast than fungi (2,4.105 vs 3,4.103 germes/ml respectively). Fecal streptococci were detected in 43,14% of the samples were E.coli is detected in 17,65% of the milk. In addition, 28% of samples are positive for Clostridium against 9,8% for Staphylococcus aureus. All milk are free from Salmonella. In terms of Brucella antibody, 13,73% of milk are ring test positive. The poor quality of milk reflects the deplorable conditions of production and non-observance of good hygiene practices during milking.Keywords : Ewe, Raw milk, Hygienic quality, Pathogens, Algeria

A Prototype Atom Interferometer to Detect Dark Matter and Gravitational Waves

The AION project has built a tabletop prototype of a single-photon long-baseline atom interferometer using the 87Sr clock transition - a type of quantum sensor designed to search for dark matter and gravitational waves. Our prototype detector operates at the Standard Quantum Limit (SQL), producing a signal with no unexpected noise beyond atom shot noise. Importantly, the detector remains at the SQL even when additional laser phase noise is introduced, emulating conditions in a long-baseline detector such as AION or AEDGE where significant laser phase deviations will accumulate during long atom interrogation times. Our results mark a key milestone in extending atom interferometers to long baselines. Such interferometers can complement laser-interferometer gravitational wave detectors by accessing the mid-frequency gravitational wave band around 1 Hz, and can search for physics beyond the Standard Model

A Prototype Atom Interferometer to Detect Dark Matter and Gravitational Waves

The AION project has built a tabletop prototype of a single-photon long-baseline atom interferometer using the 87Sr clock transition - a type of quantum sensor designed to search for dark matter and gravitational waves. Our prototype detector operates at the Standard Quantum Limit (SQL), producing a signal with no unexpected noise beyond atom shot noise. Importantly, the detector remains at the SQL even when additional laser phase noise is introduced, emulating conditions in a long-baseline detector such as AION or AEDGE where significant laser phase deviations will accumulate during long atom interrogation times. Our results mark a key milestone in extending atom interferometers to long baselines. Such interferometers can complement laser-interferometer gravitational wave detectors by accessing the mid-frequency gravitational wave band around 1 Hz, and can search for physics beyond the Standard Model

Profils en acides gras libres, cholestérol et indices lipidiques du lait cru ovin issu de deux races algériennes (Ouled-Djellal et Rumbi) collecté en milieu steppique

Free fatty acid profiles and cholesterol and lipid indexes of raw ewe's milk from two Algerian breeds (Ouled-Djellal and Rumbi), collected in the steppe area. Description of the subject. This article aims to characterize the fat content of raw ewe's milk, collected locally in the Algerian central steppe, an area highly appreciated for its production of smen (traditional butter). Objectives. The objective of this study was to investigate the free fatty acid profile and the cholesterol and lipid indexes of raw ewe's milk from two Algerian local breeds (Ouled-Djellal and Rumbi). Method. Milk was collected from 20 ewes of each breed three times during the middle stage of lactation. The milk fat was extracted using the Rose-Gottlieb method. Fatty acids were analyzed by gas chromatography. Results. Palmitic (24.4% ± 3.3), oleic (24.2% ± 4.6), stearic (10.8% ± 3.5), myristic (10.4% ± 1.9) and capric (8.3% ± 3.5) acids dominated and accounted for 78.1% of the total fatty acids. Saturated fatty acids predominated compared to unsaturated fatty acids (69.6% ± 5.3 vs 30.8% ± 5.1), and monounsaturated fatty acids outweighed the polyunsaturated (26.3% ± 4.7 vs 4.5% ± 1.1). The Ouled-Djellal milk had a higher short-chain fatty acid content than the Rumbi milk (p < 0.05). On the other hand, the Rumbi milk was richer in medium chain fatty acids. The two types of milk differed significantly regarding their palmitic and palmitoleic acid content (p < 0.05). Within the long chain of fatty acids, only the stearic acid (10.88% ± 2.83 vs 12.34% ± 2.75; p < 0.01) and the arachidic acid (0.26% ± 0.18 vs 0.35% ± 0.09; p < 0.05) were influenced by the breed. Conclusions. Results showed that milk from the Rumbi ewe was richer in fatty acids than the milk from the Ouled-Djellal and that Rumbi ewe's milk may be of value in dairy technology due to the socio-economic impact that it can generate

Inflammation and sepsis as part of hematopoietic stem cell transplantation: a transcriptomic approach

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Reactive oxygen species and aldehyde dehydrogenase 1A as prognosis and theragnostic biomarker in acute myeloid leukaemia patients

International audiencecute myeloid leukaemia (AML) remains a major unmet medical, despite recent progress in targeted molecular therapies. One aspect of leukaemic cell resistance to chemotherapy is the development of clones with increased capacity to respond to cellular stress and the production of reactive oxygen species (ROS), thanks in particular to a high aldehyde dehydrogenases (ALDH) 1A1/2 activity. At diagnosis, ROS level and ALDH1A1/2 activity in AML patients BM are correlated with the different ELN 2022 prognostic groups and overall survival (OS). A significant lower ALDH1A1/2 activity in BM was observed in the favourable ELN2022 subgroup compared to the intermediate and adverse group ( p &lt; 0.01). In the same way, the ROS levels were significantly lower in the favourable ELN 2022 subgroup compared to the intermediate group ( p &lt; 0.0001) and adverse group ( p &lt; 0.0002). ROS high AML patients had a significantly lower median overall survival (OS) (8.2 months) than ROS low patients (24.6 months) ( p = 0.0368). After first‐line therapy, a significant increase of ROS level ( p = 0.015) and ALDH1A1/2 activity (0 = 0.0273) in leukaemic blasts was observed, especially in the refractory ones. ABD‐3001, a competitive and irreversible inhibitor of ALDHs 1 and 3, can in vitro inhibit the proliferation of patient‐derived leukaemic cells in accordance with redox balance. In multivariate analysis, ROS level was the most significant ( p &lt; 0.05) and the strongest predictive factor for the sensitivity of cells to ABD‐3001. The safety profile of ABD‐3001 is currently being assessed through the first inhuman multicenter phase 1 clinical trial “ODYSSEY” (NCT05601726) for patients with relapsed AML

Aldehyde dehydrogenases inhibition eradicates leukemia stem cells while sparing normal progenitors

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