The Effects of a Non-Ferroelectric Slab on the Polarization and the Susceptibility of the Ferroelectric Multilayer

The polarization and the susceptibility of a ferroelectric multilayer with a non-ferroelectric slab are investigated within the framework of transverse Ising model with a four-spin interaction term. The effect of the thickness and the position of the non-ferroelectric slab are investigated in this paper. We find that the increase of the thickness of the non-ferroelectric will decrease the polarization and the susceptibility of the film. If the position of the non-ferroelcetric slab shifts from the center of the film to the surface, the number of the peaks of the susceptibility will change. And a step-like polarization curve is found.Comment: 15 pages, 4 figure

A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations:implications for peptide-based analgesics

Inhibition of the NGF/TrkA interaction presents an interesting alternative to the use of non-steroidal anti-inflammatories and/or opioids for the control of inflammatory, chronic and neuropathic pain. Most prominent of the current approaches to this therapy is the antibody Tanezumab, which is a late-stage development humanized monoclonal antibody that targets NGF. We sought to determine whether peptides might similarly inhibit the NGF/TrkA interaction and so serve as future therapeutic leads. Starting from two peptides that inhibit the NGF/TrkA interaction, we sought to eliminate a cysteine residue close to the C-terminal of both sequences, by an approach of mutagenic analysis and saturation mutagenesis of mutable residues. Elimination of cysteine from a therapeutic lead is desirable to circumvent manufacturing difficulties resulting from oxidation. Our analyses determined that the cysteine residue is not required for NGF binding, but is essential for inhibition of the NGF/TrkA interaction at pharmacologically relevant peptide concentrations. We conclude that a cysteine residue is required within potential peptide-based therapeutic leads and hypothesise that these peptides likely act as dimers, mirroring the dimeric structure of the TrkA receptor

A2BR Adenosine Receptor Modulates Sweet Taste in Circumvallate Taste Buds

In response to taste stimulation, taste buds release ATP, which activates ionotropic ATP receptors (P2X2/P2X3) on taste nerves as well as metabotropic (P2Y) purinergic receptors on taste bud cells. The action of the extracellular ATP is terminated by ectonucleotidases, ultimately generating adenosine, which itself can activate one or more G-protein coupled adenosine receptors: A1, A2A, A2B, and A3. Here we investigated the expression of adenosine receptors in mouse taste buds at both the nucleotide and protein expression levels. Of the adenosine receptors, only A2B receptor (A2BR) is expressed specifically in taste epithelia. Further, A2BR is expressed abundantly only in a subset of taste bud cells of posterior (circumvallate, foliate), but not anterior (fungiform, palate) taste fields in mice. Analysis of double-labeled tissue indicates that A2BR occurs on Type II taste bud cells that also express Gα14, which is present only in sweet-sensitive taste cells of the foliate and circumvallate papillae. Glossopharyngeal nerve recordings from A2BR knockout mice show significantly reduced responses to both sucrose and synthetic sweeteners, but normal responses to tastants representing other qualities. Thus, our study identified a novel regulator of sweet taste, the A2BR, which functions to potentiate sweet responses in posterior lingual taste fields

Tensile strength of Silicon Nanowires batch-fabricated into electrostatic MEMS testing device

The tensile strength of a silicon nanowire (SiNW) that had been integrated into a silicon-on-insulator (SOI)-based microelectromechanical system (MEMS) device was measured using electrostatic actuation and sensing. SiNWs of about 150 nm diameter and 5 μm length were batch-fabricated into a 5-μm-thick SOI device layer. Since there was no interface between the SiNW and the MEMS device and the alignment was perfect, the SiNW integration into an SOI-MEMS was expected to be useful for developing highly sensitive biochemical sensors or highly reliable torsional mirror devices. The SiNW was tensile tested using the electrostatic MEMS testing device. The integration was achieved using a combination of anisotropic and an isotropic dry etching of silicon, with an inductively coupled plasma reactive ion etching. A fabricated silicon beam of 800 nm square was thinned by a sacrificial oxidation process. The tensile strength of the wire was 2.6–4.1 GPa, which was comparable to that of microscale silicon MEMS structures. The reliability of such a thin device was successfully verified for future applications of the device structures

Differential effect of chronic aflatoxin B1 intoxication on the growth performance and incidence of hepatic lesions in triploid and diploid rainbow trout (Oncorhynchus mykiss)

Triploid trout has been considered to be more resistant than diploid trout to many diseases and to some adverse aquaculture conditions. Considering the common problems with animal food contamination by aflatoxins, the purpose of this research was to compare the incidence of liver lesions and growth performance in triploid and diploid trout (O. mykiss) exposed to chronic contamination with aflatoxin B1. A total of 240 samples formed 4 groups, diploid and triploid control without aflatoxin in food and diploid and triploid treated with 80 ppb of aflatoxin/Kg of food. Five samples in each group were monthly sacrificed during one year. After the measurement of body weight and length, hepatic samples were obtained and fixed in 10% formalin-saline solution. The histopathological analyses were performed with liver slides stained with hematoxylin and eosin. The comparative analyses of the growth performance showed significant difference between control and treated diploid, suggesting that aflatoxin B1 affects the growing of diploid trout. In triploid groups this aspect was not observed. The histopathological analyses indicated that triploid trout is more resistant to aflatoxin B1, since this group did not present neoplastic lesions. Diploid fishes however, showed 4 samples with neoplastic lesion. The triploid treated group had preneoplastic lesion, but with minor incidence and slower progression than diploid trout.34225326

Tensile fracture of integrated single-crystal silicon nanowire using MEMS electrostatic testing device

AbstractTensile testing of single-crystal silicon (SCS) nanowire integrated into electrostatic micro electro mechanical system (MEMS) device was conducted. The nanowire was fabricated using batch process for future integration of nanowires to MEMS sensors or actuators. The tensile specimen of SCS nanowire has a circular cross section of 100 to 200 nm in diameter, 5 μm long. The diameter was controlled by oxidizing 800-nm square cross-section wires fabricated using electron beam lithography. The oxidizing thinning process also reduced the surface roughness. On-chip tensile testing using an electrostatic actuator and sensor was conducted for the specimen of 190 nm in cross-section size. The tensile strength of the wire was 2.6 GPa. The strength and fracture properties were discussed by comparing with a silicon nanowire fabricated using two-step Bosch process to examine the difference in surface finishing

Relationships among growth and different NOR phenotypes in a specific stock of rainbow trout (Oncorhynchus mykiss)

Growth is one of the most important aspects in the genetic improvement of cultured fish species. Consequently, genetic parameters related to this feature and their response to selection have been the focus of most research in this area. Such research indicates that, in general, there is enough additive genetic variance related to growth, justifying the use of selection. Based on the usefulness of cytogenetic and molecular markers in the fish culture, the aim of the present work was to analyze the possible relationships among cytogenetic characteristics, specifically the NOR phenotypes, and the increase in length and weight in specimens of the rainbow trout (Oncorhynchus mykiss), resultant from directed mating between homozygous females and heterozygous males according to their NOR phenotypic patterns. The equations of the relationship between length and weight of the analyzed specimens followed the model Wt = a Lt b, showing b values higher than 3, determinant of a positive allometric growth. The results showed that the different NOR phenotypes were not related with the growth values for length and weight in any statistical test

Relationships among growth and different NOR phenotypes in a specific stock of rainbow trout (Oncorhynchus mykiss)

Growth is one of the most important aspects in the genetic improvement of cultured fish species. Consequently, genetic parameters related to this feature and their response to selection have been the focus of most research in this area. Such research indicates that, in general, there is enough additive genetic variance related to growth, justifying the use of selection. Based on the usefulness of cytogenetic and molecular markers in the fish culture, the aim of the present work was to analyze the possible relationships among cytogenetic characteristics, specifically the NOR phenotypes, and the increase in length and weight in specimens of the rainbow trout (Oncorhynchus mykiss), resultant from directed mating between homozygous females and heterozygous males according to their NOR phenotypic patterns. The equations of the relationship between length and weight of the analyzed specimens followed the model Wt = a Lt b, showing b values higher than 3, determinant of a positive allometric growth. The results showed that the different NOR phenotypes were not related with the growth values for length and weight in any statistical test