Research on the Application of "Internet Plus" Rehabilitation Nursing in Children with Cerebral Palsy

Abstract: Currently, cerebral palsy has emerged as a severe disease deserving significant attention. Amidst the thriving development of internet technology, the "Internet+" platform has brought new support to the medical field. This study aims to explore the application effects of "Internet+"-based rehabilitation nursing for children with cerebral palsy. A total of 60 children with cerebral palsy were randomly divided into a control group and an experimental group. The control group received conventional rehabilitation nursing, while the experimental group underwent "Internet+"-based rehabilitation nursing intervention. Comparisons were made between the two groups in terms of overall disease recovery, fine motor function, gross motor function, activities of daily living (ADL), nursing satisfaction, and quality of life. The results showed that the experimental group surpassed the control group in motor function, ADL, disease recovery efficiency, nursing satisfaction, and quality of life assessments, with statistically significant differences (P<0.05). The application of "Internet+"-based rehabilitation nursing for children with cerebral palsy can lead to more desirable overall nursing outcomes. &nbsp

Effectiveness Observation of Oral Motor Training Games in the Rehabilitation of Children with Articulation Disorders

Oral motor training games provide a novel reference for rehabilitation in children with articulation disorders. This study investigates the application effects of this method. Sixty children undergoing rehabilitation for articulation disorders were randomly divided into an observation group and a control group. The control group received conventional rehabilitation interventions, while the observation group incorporated oral motor training games into the baseline protocol. Comparisons were made between the two groups regarding improvements in oral motor function and speech abilities. Results demonstrated that the observation group exhibited significantly higher scores in both oral motor function and speech function compared to the control group (P < 0.05)23. Compared to conventional methods, oral motor training games show distinct advantages in enhancing articulation abilities, oral motor coordination, and speech outcomes for children with articulation disorders56. These findings suggest that oral motor training games can serve as a scientifically validated and effective rehabilitation intervention, offering valuable insights for clinical practice in articulation disorder management. &nbsp

Characteristic analysis of lightning activities on the Yungui Plateau using ground-based remote sensing

The spatiotemporal distribution of cloud-to-ground (CG) lightning activities on the Yungui Plateau is investigated in this study by using a 5-year dataset (2016–2020) from the ground-based National Lightning Detection Network (CNLDN). The correlations between the lightning activities and different meteorological factors in the region are also analyzed. The results show that there is an obvious difference in the spatial distribution of lightning activities on the Yungui Plateau during the 5 years, with high lightning density in the east and low lightning density in the west. The lightning activities shift and gather more towards the eastern plateau especially after 2019. Affected by the quasi-stationary front in Kunming, the spatial distributions of lightning flashes in cold and warm seasons are different. On the other hand, the frequency of the lightning activities varies from year to year, such as the surge in 2019. But in general, 62% of the lightning activities are produced in summer and the lightning flashes occur more often in the afternoon and evening on the Yungui Plateau. Additionally, it is found that lightning activities in the 5 years are closely related to precipitation and temperature, while there is a weak correlation with relative humidity and almost no correlation with sensible heat flux. The analysis also indicates that the CAPE×P (convective available potential energy times precipitation rate) proxy can be effectively used to describe and predict lightning activities on the Yungui Plateau as the lightning flashes corresponds well to CAPE×P, especially of the spatial distribution

miR-27a-3p regulates intestinal cell proliferation and differentiation through Wnt/β-catenin signalling

Intestinal stem cells differentiate into absorptive enterocytes, characterised by increased brush border enzymes such as intestinal alkaline phosphatase (IAP), making up the majority (95%) of the terminally differentiated cells in the villus. Loss of integ- rity of the intestinal epithelium plays a key role in inflammatory diseases and gastro- intestinal infection. Here, we show that the intestinal microRNA (miR)-27a-3p is an important regulator of intestinal epithelial cell proliferation and enterocyte differenti- ation. Repression of endogenous miR-27a-3p leads to increased enterocyte differen- tiation and decreased intestinal epithelial cell proliferation in mouse and human small intestinal organoids. Mechanistically, miR-27a-3p regulates intestinal cell differentia- tion and proliferation at least in part through the regulation of retinoic acid receptor α (RXRα), a modulator of Wnt/β-catenin signalling. Repression of miR-27a-3p increases the expression of RXRα and concomitantly, decreases the expression of active β-catenin and cyclin D1. In contrast, overexpression of miR-27a-3p mimic decreases the expression of RXRα and increases the expression of active β-catenin and cyclin D1. Moreover, overexpression of the miR-27a-3p mimic results in impaired enterocyte differentiation and increases intestinal epithelial cell prolifera- tion. These alterations were attenuated or blocked by Wnt inhibition. Our study demonstrates an miR-27a-3p/RXRα/Wnt/β-catenin pathway that is important for the maintenance of enterocyte homeostasis in the small intestine

A General Model for Analyzing the Thermal Characteristics of a Class of Latent Heat Thermal Energy Storage Systems

The present study describes and classifies latent heat thermal energy storage (LHTES

A comprehensive approach to developing a multi-epitope vaccine against Mycobacterium tuberculosis: from in silico design to in vitro immunization evaluation

IntroductionThe Bacillus Calmette-Guérin (BCG) vaccine, currently used against tuberculosis (TB), exhibits inconsistent efficacy, highlighting the need for more potent TB vaccines.Materials and methodsIn this study, we employed reverse vaccinology techniques to develop a promising multi-epitope vaccine (MEV) candidate, called PP13138R, for TB prevention. PP13138R comprises 34 epitopes, including B-cell, cytotoxic T lymphocyte, and helper T lymphocyte epitopes. Using bioinformatics and immunoinformatics tools, we assessed the physicochemical properties, structural features, and immunological characteristics of PP13138R.ResultsThe vaccine candidate demonstrated excellent antigenicity, immunogenicity, and solubility without any signs of toxicity or sensitization. In silico analyses revealed that PP13138R interacts strongly with Toll-like receptor 2 and 4, stimulating innate and adaptive immune cells to produce abundant antigen-specific antibodies and cytokines. In vitro experiments further supported the efficacy of PP13138R by significantly increasing the population of IFN-γ+ T lymphocytes and the production of IFN-γ, TNF-α, IL-6, and IL-10 cytokines in active tuberculosis patients, latent tuberculosis infection individuals, and healthy controls, revealing the immunological characteristics and compare the immune responses elicited by the PP13138R vaccine across different stages of Mycobacterium tuberculosis infection.ConclusionThese findings highlight the potential of PP13138R as a promising MEV candidate, characterized by favorable antigenicity, immunogenicity, and solubility, without any toxicity or sensitization

Laboratory investigation of the first infant botulism case caused by type E botulinum neurotoxin producing Clostridium butyricum in China

Objective Laboratory investigation was performed on a suspected case of infant botulism. Methods Thirty samples of stool, left-over food and environment swabs related to the case were collected, Clostridium spp. isolation, identification and toxicity determination by mouse assay were carried out, and toxin production for isolate was conducted. Results Mice injected with the stool culture supernatant showed the typical signs of botulism including irritable, dyspnea, bellows breathing and quadriplegia followed by death. The toxicity of the stool culture supernatant was enhanced after the treatment by trypsinization but ceased after being heated at 100℃. The polyvalent antibody against botulinum neurotoxins (BoNTs) and the monovalent antibody against BoNT type E could protect mice from death. One gram-positive Clostridium isolate was cultured from infant stool sample. The morphology of the colony on the Columbia blood agar plate showed characteristics of irregular, translucent and flat with rootlike growth. It was positive for type E BoNT-encoding gene and identified as C. butyricum by 16S rRNA sequencing. Toxin production test illustrated that the C. butyricum isolate could produce type E BoNT. Conclusion This was an infant botulism caused by type E BoNT-producing C. butyricum

Structural basis for the ligand-dependent activation of heterodimeric AHR-ARNT complex

The aryl hydrocarbon receptor (AHR) possesses an extraordinary capacity to sense and respond to a wide range of small-molecule ligands, ranging from polycyclic aromatic hydrocarbons to endogenous compounds. Upon ligand binding, AHR translocates from the cytoplasm to nucleus, forming a transcriptionally active complex with aryl hydrocarbon receptor nuclear translocator (ARNT), for DNA binding and initiation of gene expression programs that include cellular detoxification pathways and immune responses. Here, we examine the molecular mechanisms governing AHR’s high-affinity binding and activation by a diverse group of ligands. Crystal structures of the AHR-ARNT-DNA complexes, bound with each of six established AHR ligands, including Tapinarof, 6-formylindolo[3,2-b]carbazole (FICZ), benzo[a]pyrene (BaP), β-naphthoflavone (BNF), Indigo and Indirubin, reveal an unconventional mode of subunit assembly with intimate association between the PAS-B domains of AHR and ARNT. AHR’s PAS-B domain utilizes eight conserved residues whose dynamic rearrangements account for the ability to bind to ligands through hydrophobic and π-π interactions. Our findings further reveal the structural underpinnings of a ligand-driven activation mechanism, whereby a segment of the AHR protein undergoes a structural transition from chaperone engagement to ARNT heterodimer stabilization, to generate the transcriptionally competent assembly. Our results provide key information for the future development of AHR-targeting drugs

Serotype distribution and antimicrobial resistance characteristics of Salmonella in chicken meat collected from wholesale markets in Beijing City

ObjectiveTo investigate the serotype distribution and antimicrobial resistance characteristics of Salmonella isolated from retail chicken in Beijing City.MethodsA total of 135 Salmonella strains were isolated from retail chicken meat collected from three large wholesale markets in Fengtai District in Beijing City from 2014 to 2015. The serotype of these Salmonella strains was identified using both a Luminex serotyping kit and a traditional serum agglutination method. Multilocus sequence typing was performed according to the recommended conditions of the Enterobase database. Antimicrobial susceptibility testing plates were used to determine the susceptibility of the isolates against 12 classes of drugs representing 25 antimicrobial agents. PCR was used to detect the prevalence of the 10 mobile colistin resistance genes （mcr） and three NDM-type carbapenemases genes （blaNDM）.ResultsAmong the 135 Salmonella strains， 6 serogroups were detected， with D1 and B being the main serogroups； 20 serotypes were detected， in which Enteritidis， Indiana， and Thompson were the dominant ones with percentages of 50.4%， 15.6%， and 8.1%， respectively； 21 ST types were obtained， and a good correspondence was observed between serotypes and ST types. 91.9% of all tested isolates showed resistance to the tested drugs and showed the highest resistance to nalidixic acid， ampicillin， ampicillin-sulbactam， tetracycline. Multi-drug resistant strains accounted for 71.1% of all strains and were resistant to as many as 18 kinds of antimicrobial compounds belonging to 10 categories. Among the 17 strains co-resistant to 8 or more classes of drugs， 16 were of the Indiana serotype. The proportion of strains with high multi-drug resistance was different in different packaging methods， different seasons， and different wholesale markets. All strains were negative for the mcr and blaNDM genes.ConclusionThe serotypes of Salmonella isolates from chicken meat in wholesale markets in Beijing City from 2014 to 2015 were complex， with the dominant serotype of Salmonella Enterititis， and the overall antimicrobial resistance level of all isolates was high. To assess the possible food safety risks， continuous monitoring for Salmonella Indiana with high levels of antimicrobial resistance must be carried out and their drug resistance and transmission mechanisms must be studied to help develop first-line and ultimate drugs for clinical treatment of Salmonella diseases

Arterial stiffness in subclinical atherosclerosis quantified with ultrafast pulse wave velocity measurements: a comparison with a healthy population using propensity score matching

Purpose This study aimed to evaluate changes in ultrafast pulse wave velocity (ufPWV) in individuals with arterial stiffness and subclinical atherosclerosis (subAS), and to provide cutoff values. Methods This retrospective study recruited 231 participants, including 67 patients with subAS. The pulse wave velocity was measured at the beginning and end of systole (PWV-BS and PWVES, respectively) using ultrafast ultrasonography to assess arterial stiffness. The right and left common carotid arteries were measured separately, and laboratory metabolic parameters were also collected. Participants were balanced between groups using propensity score matching (PSM) at a 1:1 ratio, adjusting for age, sex, and waist-to-hip ratio as potential confounders. Cutoff values of ufPWV for monitoring subAS were determined via receiver operating characteristic (ROC) curve analysis. Results PWV-ES, unlike PWV-BS, was higher in the subAS subgroup than in the subAS-free group after PSM (all P0.05). Conclusion PWV-ES measured using ultrafast ultrasonography was significantly higher in individuals with subAS than in those without. Specific PWV-ES cutoff values showed potential for predicting an increased risk of subAS