Report on Validation and Calibration of Fatty Acid Signatures in Blubber as Indicators of Prey in Hawaiian Monk Seal Diet

Pacific Islands Fisheries Science Center Administrative Reports are issued to promptly disseminate scientific and technical information to marine resource managers, scientists, and the general public. Their contents cover a range of topics, including biological and economic research, stock assessment, trends in fisheries, and other subjects. Administrative Reports typically have not been reviewed outside the Center. As such, they are considered informal publications. The material presented in Administrative Reports may later be published in the formal scientific literature after more rigorous verification, editing, and peer review. Other publications are free to cite Administrative Reports as they wish provided the informal nature of the contents is clearly indicated and proper credit is given to the author(s). Administrative Reports may be cited as follows: Iverson, S. J., B. S. Stewart, and P. K. Yochem. 2010. Report on validation and calibration of fatty acid signatures in blubber as indicators o

Health Assessment of Weddell Seals, \u3ci\u3eLeptonychotes weddellii\u3c/i\u3e, in McMurdo Sound, Antarctica

The demography of Weddell seals in eastern McMurdo Sound, Antarctica, has been well studied during the past three decades (e.g. Stirling 1971; Siniff et al. 1977; Testa and Siniff 1987; Hastings and Testa 1998; Gelatt et al. 2001). Detailed life-history data are available on thousands of seals tagged as pups in McMurdo Sound, making this population a rich resource for wildlife health studies because health parameters can be evaluated in the light of reproductive histories and genetic relationships of several generations of tagged seals. Recently, evidence of exposure to diseases generally associated with domestic animals and feral wildlife has been detected in Antarctic wildlife (Austin and Webster 1993; Olsen et al. 1996; Gardner et al. 1997; Retamal et al. 2000; Foster et al. 2002) and this has generated concern and debate regarding the risks of disease introduction to Antarctic wildlife. Antibodies to viruses that have caused large die-offs in phocids in other areas of the world have been detected in Weddell seals (Bengtson et al. 1991), and there is a historical report of a mass die-off of crabeater seals that may have had a viral etiology (Laws and Taylor 1957)

The NM23-H1/H2 homolog NDK-1 is required for full activation of Ras signaling in C. elegans

The group I members of the Nm23 (non-metastatic) gene family encode nucleoside diphosphate kinases (NDPKs) that have been implicated in the regulation of cell migration, proliferation and differentiation. Despite their developmental and medical significance, the molecular functions of these NDPKs remain ill defined. To minimize confounding effects of functional compensation between closely related Nm23 family members, we studied ndk-1, the sole Caenorhabditis elegans ortholog of group I NDPKs, and focused on its role in Ras/mitogen-activated protein kinase (MAPK)-mediated signaling events during development. ndk-1 inactivation leads to a protruding vulva phenotype and affects vulval cell fate specification through the Ras/MAPK cascade. ndk-1 mutant worms show severe reduction of activated, diphosphorylated MAPK in somatic tissues, indicative of compromised Ras/MAPK signaling. A genetic epistasis analysis using the vulval induction system revealed that NDK-1 acts downstream of LIN-45/Raf, but upstream of MPK-1/MAPK, at the level of the kinase suppressors of ras (KSR-1/2). KSR proteins act as scaffolds facilitating Ras signaling events by tethering signaling components, and we suggest that NDK-1 modulates KSR activity through direct physical interaction. Our study reveals that C. elegans NDK-1/Nm23 influences differentiation by enhancing the level of Ras/MAPK signaling. These results might help to better understand how dysregulated Nm23 in humans contributes to tumorigenesis. © 2013. Published by The Company of Biologists Ltd

Aerial Surveys for Cetaceans in the Former Akutan, Alaska, Whaling Grounds

Randomized aerial surveys were flown between 26 July and 26 August 1984 to search for cetaceans in two areas of southwestern Alaska: one on both Bering Sea and Pacific Ocean sides of the Aleutian Islands near the defunct Akutan shore-whaling station, which operated from 1912 through 1938, the other overlapping continental slope and shallow continental shelf waters between the Aleutians and the Pribilof Islands. ... Searches covered about 3940 nautical miles (nm), including some 2403 nm of random transects. Sightings were made of gray whales (10 sightings, 14 individuals), fin whales (3, 11), minke whales (1, 1), unidentified beaked whales (1, 6), Dall's porpoises (47, 131), killer whales (8, 26), and harbor porpoises (4, 7). A Fourier series model was used to estimate density of Dall's porpoises as 115 individuals (CV=0.263) per 1000 sq nm on the whaling grounds and 16.6 individuals (CV=0.0) per sq nm in the Bering Sea north of the whaling grounds. These estimates are comparable to those previously reported for the same general areas (97.2 animals per 1000 sq nm, SD=49.5). There were too few sightings of other cetaceans to permit calculation of meaningful density estimates. At least four species of great whales (blue, fin, humpback and sperm) were sufficiently abundant during the first four decades of this century to support significant whaling activities within about 100 sq nm of Akutan (more than 5300 whales were caught during 23 years of whaling, 1912-39). Although previous studies of the fisheries showed a downward trend in catch per unit of effort and an increase in distance traveled to take whales, whales were still being taken at relatively high rates (0.28-0.51 whales per gross catcher day) at the end of the fishery in 1939. Populations of fin, humpback, blue and sperm whales were probably significantly reduced by shore and pelagic whaling conducted widely in the North Pacific since 1939. ...Key words: aerial surveys, cetaceans, Bering Sea, North Pacific Ocean, historical whaling Des relevés aériens ont été effectués au hasard entre le 26 juillet et le 26 août 1984, afin de déterminer la présence de cétacés dans deux régions du Sud-Ouest de l'Alaska : l'une située des deux côtés des îles Aléoutiennes (du côté de la mer de Béring et du côté de l'océan Pacifique), près de ce qui fut jadis le port baleinier d'Akukan qui resta en opération de 1912 à 1939; l'autre couvrant à la fois les eaux du talus continental et celles, peu profondes, de la plate-forme continentale, entre les îles Aléoutiennes et les îles Pribilof. Les relevés furent effectuées à des altitudes comprises entre 150 et 245 m, d'un appareil d'observation Partenavia P68, muni d'un nez de plexiglas, permettant de voir dans l'axe de déplacement. Les recherches ont été effectuées sur environ 3940 milles nautiques (mn), y compris 2403 mn de recoupements au hasard. One a relevé la présence de baleines grises (10 relevés, 14 individus), de rorquals communs (3, 11), de petits rorquals (1, 1), de baleines à bec non identifiées (1, 6), de marsouins de Dall (47, 131), d'épaulards (8, 26) et de marsouins communs. On a utilisé un modèle en séries de Fourier pour déterminer approximativement la densité de marsouins de Dall à 115 individus (CV = 0.263) aux 1000 mn² dans les zones de pêche à la baleine, et à 16.6 individus (CV = 0.0) aux 1000 mn² dans la mer de Béring au nord des zones de pêche. Ces évaluations sont comparables à celles rapportées précédemment pour ces mêmes zones en général (97.2 animaux aux 1000 mn², DS = 49.5). Trop peu d'autres cétacés ont été aperçus pour justifier le calcul des densités approximatives. Durant les quarante premières années de ce siècle, il y avait au moins quatre espèces de grandes baleines (rorquals bleus, rorquals communs, rorquals à bosse et cachalots) en quantité suffisante pour alimenter une industrie baleinière dans un rayon d'environ 100 mn d'Akutan. (Plus de 5300 baleines furent pêchées durant les 23 années que dura la pêche à la baleine, de 1912 à 1939). Bien que des études précédentes sur la pêche aient montré une tendance à la baisse du nombre de prises par rapport au nombre d'unités d'effort et une augmentation de la distance à parcourir pour capturer les baleines, celles-ci étaient capturées à un taux relativement élevé (de 0.28 à 0.51 baleine par unité d'effort brute par jour) à la fin de la pêche en 1939. Les populations de rorquals communs, de rorquals à bosse, de rorquals bleus et de cachalots ont probablement été réduites de façon significative par le pêche côtière et la pêche pélagique, qui ont été pratiquées à grande échelle dans le Pacifique Nord depuis 1939. Le petit nombre de cétacés aperçus durant les présents relevés porte à croire que les populations dans les zones de pêche et dans leur vicinité, restent peu élevées en raison de ces activités. Mots clés : relevé aérien, cétacés, mer de Béring, Pacifique Nord, ancienne pêche à la balein

Bursting the Burden Bubble? An Assessment of Sharma et al.'s Counterfactual-based Fairness Metric

Machine learning has seen an increase in negative publicity in recent years, due to biased, unfair, and uninterpretable models. There is a rising interest in making machine learning models more fair for unprivileged communities, such as women or people of color. Metrics are needed to evaluate the fairness of a model. A novel metric for evaluating fairness between groups is Burden, which uses counterfactuals to approximate the average distance of negatively classified individuals in a group to the decision boundary of the model. The goal of this study is to compare Burden to statistical parity, a well-known fairness metric, and discover Burden's advantages and disadvantages. We do this by calculating the Burden and statistical parity of a sensitive attribute in three datasets: two synthetic datasets are created to display differences between the two metrics, and one real-world dataset is used. We show that Burden can show unfairness where statistical parity can not, and that the two metrics can even disagree on which group is treated unfairly. We conclude that Burden is a valuable metric, but does not replace statistical parity: it rather is valuable to use both.Comment: 11 pages, 3 figures, conference: BNAIC/BeNeLearn (https://bnaic2022.uantwerpen.be/accepted-submissions/

The EFF-1A Cytoplasmic Domain Influences Hypodermal Cell Fusions in C. elegans But Is Not Dependent on 14-3-3 Proteins.

BACKGROUND: Regulatory and biophysical mechanisms of cell-cell fusion are largely unknown despite the fundamental requirement for fused cells in eukaryotic development. Only two cellular fusogens that are not of clear recent viral origin have been identified to date, both in nematodes. One of these, EFF-1, is necessary for most cell fusions in Caenorhabditis elegans. Unregulated EFF-1 expression causes lethality due to ectopic fusion between cells not developmentally programmed to fuse, highlighting the necessity of tight fusogen regulation for proper development. Identifying factors that regulate EFF-1 and its paralog AFF-1 could lead to discovery of molecular mechanisms that control cell fusion upstream of the action of a membrane fusogen. Bioinformatic analysis of the EFF-1A isoform\u27s predicted cytoplasmic domain (endodomain) previously revealed two motifs that have high probabilities of interacting with 14-3-3 proteins when phosphorylated. Mutation of predicted phosphorylation sites within these motifs caused measurable loss of eff-1 gene function in cell fusion in vivo. Moreover, a human 14-3-3 isoform bound to EFF-1::GFP in vitro. We hypothesized that the two 14-3-3 proteins in C. elegans, PAR-5 and FTT-2, may regulate either localization or fusion-inducing activity of EFF-1. METHODOLOGY/PRINCIPAL FINDINGS: Timing of fusion events was slightly but significantly delayed in animals unable to produce full-length EFF-1A. Yet, mutagenesis and live imaging showed that phosphoserines in putative 14-3-3 binding sites are not essential for EFF-1::GFP accumulation at the membrane contact between fusion partner cells. Moreover, although the EFF-1A endodomain was required for normal rates of eff-1-dependent epidermal cell fusions, reduced levels of FTT-2 and PAR-5 did not visibly affect the function of wild-type EFF-1 in the hypodermis. CONCLUSIONS/SIGNIFICANCE: Deletion of the EFF-1A endodomain noticeably affects the timing of hypodermal cell fusions in vivo. However, prohibiting phosphorylation of candidate 14-3-3-binding sites does not impact localization of the fusogen. Hypodermal membrane fusion activity persists when 14-3-3 expression levels are reduced

Utilization of the Clinical Laboratory for the Implementation of Concussion Biomarkers in Collegiate Football and the Necessity of Personalized and Predictive Athlete Specific Reference Intervals

Background: A continued interest in concussion biomarkers makes the eventual implementation of identified biomarkers into routine concussion assessment an eventual reality. We sought to develop and test an interdisciplinary approach that could be used to integrate blood-based biomarkers into the established concussion management program for a collegiate football team. Methods: We used a CLIA-certified laboratory for all testing and chose biomarkers where clinically validated testing was available as would be required for results used in clinical decision making. We summarized the existing methods and results for concussion assessment across an entire season to identify and demonstrate the challenges with the eventual integration of a parallel process using blood-based tests for concussion management. We analyzed the results of the biomarkers chosen for trends consistent with the outcome assessments provided from the current concussion management protocols. Results: Baseline samples were collected with three additional post-concussion samples collected at three separate time points from players with a diagnosed concussion (n = 12). A summary of results from currently used concussion assessment tools were compared to the representative biomarkers S100B and NSE results. Nine sport-related concussions occurred during practice and three during play. For S100B, 50 % had follow-up testing results lower than the post-injury result. In contrast, 92 % of NSE follow-up results were lower than post-injury. One hundred percent of the results for S100B and NSE were within the athlete-derived reference intervals upon return-to-play and season end. Conclusions: The reported workflow provides a framework for the eventual implementation of biomarkers for concussion assessment into existing assessment protocols and strengthens the need for reliance on clinical laboratory testing. Athlete-specific reference intervals will be required to adequately interpret results

Carboxy-Terminal Truncation Activates glp-1 Protein to Specify Vulval Fates in Caenorhabditis elegans

The glp-1 and lin-12 genes encode homologous transmembrane proteins that may act as receptors for cell interactions during development. The glp-1 product is required for induction of germ-line proliferation and for embryogenesis. By contrast, lin-12 mediates somatic cell interactions, including those between the precursor cells that form the vulval hypodermis (VPCs). Here we analyse an unusual allele of glp-1, glp-1(q35), which displays a semidominant multivulva phenotype (Muv), as well as the typical recessive, loss-of-function Glp phenotypes (sterility and embryonic lethality). We find that the effects of glp-1(q35) on VPC development mimic those of dominant lin-12 mutations, even in the absence of lin-12 activity. The glp-1(q35) gene bears a nonsense mutation predicted to eliminate the 122 C-terminal amino acids, including a ProGluSerThr (PEST) sequence thought to destabilize proteins. We suggest that the carboxy terminus bears a negative regulatory domain which normally inactivates glp-1 in the VPCs. We propose that inappropriate glp-1(q35) activity can substitute for lin-12 to determine vulval fate, perhaps by driving the VPCs to proliferate

Health Assessment of Weddell Seals, Leptonychotes weddellii, in McMurdo Sound, Antarctica

This Article is brought to you for free and open access by the US Department of Commerce at DigitalCommons@University of Nebraska- Lincoln. I

Visualization of C. elegans transgenic arrays by GFP

BACKGROUND: Targeting the green fluorescent protein (GFP) via the E. coli lac repressor (LacI) to a specific DNA sequence, the lac operator (lacO), allows visualization of chromosomes in yeast and mammalian cells. In principle this method of visualization could be used for genetic mosaic analysis, which requires cell-autonomous markers that can be scored easily and at single cell resolution. The C. elegans lin-3 gene encodes an epidermal growth factor family (EGF) growth factor. lin-3 is expressed in the gonadal anchor cell and acts through LET-23 (transmembrane protein tyrosine kinase and ortholog of EGF receptor) to signal the vulval precursor cells to generate vulval tissue. lin-3 is expressed in the vulval cells later, and recent evidence raises the possibility that lin-3 acts in the vulval cells as a relay signal during vulval induction. It is thus of interest to test the site of action of lin-3 by mosaic analysis. RESULTS: We visualized transgenes in living C. elegans by targeting the green fluorescent protein (GFP) via the E. coli lac repressor (LacI) to a specific 256 sequence repeat of the lac operator (lacO) incorporated into transgenes. We engineered animals to express a nuclear-localized GFP-LacI fusion protein. C. elegans cells having a lacO transgene result in nuclear-localized bright spots (i.e., GFP-LacI bound to lacO). Cells with diffuse nuclear fluorescence correspond to unbound nuclear localized GFP-LacI. We detected chromosomes in living animals by chromosomally integrating the array of the lacO repeat sequence and visualizing the integrated transgene with GFP-LacI. This detection system can be applied to determine polyploidy as well as investigating chromosome segregation. To assess the GFP-LacI•lacO system as a marker for mosaic analysis, we conducted genetic mosaic analysis of the epidermal growth factor lin-3, expressed in the anchor cell. We establish that lin-3 acts in the anchor cell to induce vulva development, demonstrating this method's utility in detecting the presence of a transgene. CONCLUSION: The GFP-LacI•lacO transgene detection system works in C. elegans for visualization of chromosomes and extrachromosomal transgenes. It can be used as a marker for genetic mosaic analysis. The lacO repeat sequence as an extrachromosomal array becomes a valuable technique allowing rapid, accurate determination of spontaneous loss of the array, thereby allowing high-resolution mosaic analysis. The lin-3 gene is required in the anchor cell to induce the epidermal vulval precursors cells to undergo vulval development