Acetylcholine Contributes to Head Direction Cell Stability During Path Integration and Landmark Navigation

Perceived directional heading is represented in the brain by head direction (HD) cells, which fire rapidly when the head is pointed in one direction and become virtually silent when the head is pointed in all other directions. The HD signal is dominantly controlled by the position of visual landmarks, but can be maintained by path integration when familiar landmarks are not available. The neural mechanism(s) that allow path integration to maintain the HD signal have not been investigated, but a possible component of this mechanism is acetylcholine, given that selective cholinergic lesions impair path integration-based navigation. To test this, we recorded HD cell activity from the anterodorsal thalamus while rats foraged for food within a cylinder, or navigated within a dual chamber apparatus, after systemic injection of saline or atropine sulfate. In the cylinder, a prominent cue card served as the sole landmark for a standard session, after which the cue was removed for a no-cue session. Saline or atropine sulfate was then injected, and a second no-cue session was conducted, followed by standard, 90° cue rotation, standard, and no-cue sessions. During the first no-cue session after injection, some cells in atropine-treated rats showed slightly more drift in preferred firing direction (PFD) than control cells, but otherwise appeared to be unaffected by atropine. With the cue rotated 90º, 10 of the 19 (53%) cells in atropine-treated rats and 12 of the 17 (71%) control cells shifted within ± 30° of 90º. In the dual chamber apparatus, rats walked from a familiar cylinder to a novel rectangle via an alleyway, and then returned to the familiar cylinder. Control HD cells (n = 7) showed a slight PFD shift as the rat entered the novel rectangle (mean absolute shift = 17.14 ± 3.80°, range = -30 to 12°), suggesting the HD signal was maintained relatively well between arenas by path integration; upon return, the PFD returned to that of the first session (mean absolute shift = 5.14 ± 1.56°, range = -12 to 6°). In contrast, 7 of the 9 HD cells in atropine-treated rats (78%) showed greater PFD shifts between the familiar cylinder and novel rectangle (mean absolute shift = 86.00 ± 12.17°, angular shift range = -102 to 114°) and between the first and last sessions in the familiar cylinder (mean absolute shift = 24.00 ± 10.16°, angular shift range = 0 to -72°); 2 of the 9 cells (22%) showed considerable PFD drift during the novel rectangle or return cylinder sessions. Thus, acetylcholine is not critical for normal HD cell activity within a familiar environment, but facilitates the stability of the HD signal during both path integration and landmark navigation

Extrasolar planets and brown dwarfs around A-F type stars - VII. Theta Cygni radial velocity variations: planets or stellar phenomenon?

(abridged) In the frame of the search for extrasolar planets and brown dwarfs around early-type main-sequence stars, we present the results obtained on the early F-type star Theta Cygni. Elodie and Sophie at OHP were used to obtain the spectra. Our dedicated radial-velocity measurement method was used to monitor the star's radial velocities over five years. We also use complementary, high angular resolution and high-contrast images taken with PUEO at CFHT. We show that Theta Cygni radial velocities are quasi-periodically variable, with a ~150-day period. These variations are not due to the ~0.35-Msun stellar companion that we detected in imaging at more than 46 AU from the star. The absence of correlation between the bisector velocity span variations and the radial velocity variations for this 7 km/s vsini star, as well as other criteria indicate that the observed radial velocity variations are not due to stellar spots. The observed amplitude of the bisector velocity span variations also seems to rule out stellar pulsations. However, we observe a peak in the bisector velocity span periodogram at the same period as the one found in the radial velocity periodogram, which indicates a probable link between these radial velocity variations and the low amplitude lineshape variations which are of stellar origin. Long-period variations are not expected from this type of star to our knowledge. If a stellar origin (hence of new type) was to be confirmed for these long-period radial velocity variations, this would have several consequences on the search for planets around main-sequence stars, both in terms of observational strategy and data analysis. An alternative explanation for these variable radial velocities is the presence of at least one planet of a few Jupiter masses orbiting at less than 1 AU. (abridged)Comment: 9 pages, accepted in A

Alignment dependent chemisorption of vibrationally excited CH4(ν3) on Ni(100), Ni(110), and Ni(111)

International audienceWe present a stereodynamics study of the dissociative chemisorption of vibrationally excited methane on the (100), (110), and (111) planes of a nickel single crystal surface. Using linearly polarized infrared excitation of the antisymmetric C-H stretch normal mode vibration (ν3), we aligned the angular momentum and C-H stretch amplitude of CH4(ν3) in the laboratory frame and measured the alignment dependence of state-resolved reactivity of CH4 for the ν3 = 1, J = 0-3 quantum states over a range of incident translational energies. For all three surfaces studied, in-plane alignment of the C-H stretch results in the highest dissociation probability and alignment along the surface normal in the lowest reactivity. The largest alignment contrast between the maximum and minimum reactivity is observed for Ni(110), which has its surface atoms arranged in close-packed rows separated by one layer deep troughs. For Ni(110), we also probed for alignment effects relative to the direction of the Ni rows. In-plane C-H stretch alignment perpendicular to the surface rows results in higher reactivity than parallel to the surface rows. The alignment effects on Ni(110) and Ni(100) are independent of incident translational energy between 10 and 50 kJ/mol. Quantum state-resolved reaction probabilities are reported for CH4(ν3) on Ni(110) for translational energies between 10 and 50 kJ/mol

Advancing Tests of Relativistic Gravity via Laser Ranging to Phobos

Phobos Laser Ranging (PLR) is a concept for a space mission designed to advance tests of relativistic gravity in the solar system. PLR's primary objective is to measure the curvature of space around the Sun, represented by the Eddington parameter $\gamma$, with an accuracy of two parts in $10^7$, thereby improving today's best result by two orders of magnitude. Other mission goals include measurements of the time-rate-of-change of the gravitational constant, $G$ and of the gravitational inverse square law at 1.5 AU distances--with up to two orders-of-magnitude improvement for each. The science parameters will be estimated using laser ranging measurements of the distance between an Earth station and an active laser transponder on Phobos capable of reaching mm-level range resolution. A transponder on Phobos sending 0.25 mJ, 10 ps pulses at 1 kHz, and receiving asynchronous 1 kHz pulses from earth via a 12 cm aperture will permit links that even at maximum range will exceed a photon per second. A total measurement precision of 50 ps demands a few hundred photons to average to 1 mm (3.3 ps) range precision. Existing satellite laser ranging (SLR) facilities--with appropriate augmentation--may be able to participate in PLR. Since Phobos' orbital period is about 8 hours, each observatory is guaranteed visibility of the Phobos instrument every Earth day. Given the current technology readiness level, PLR could be started in 2011 for launch in 2016 for 3 years of science operations. We discuss the PLR's science objectives, instrument, and mission design. We also present the details of science simulations performed to support the mission's primary objectives.Comment: 25 pages, 10 figures, 9 table

Primary cilia elongation in response to interleukin-1 mediates the inflammatory response

Primary cilia are singular, cytoskeletal organelles present in the majority of mammalian cell types where they function as coordinating centres for mechanotransduction, Wnt and hedgehog signalling. The length of the primary cilium is proposed to modulate cilia function, governed in part by the activity of intraflagellar transport (IFT). In articular cartilage, primary cilia length is increased and hedgehog signaling activated in osteoarthritis (OA). Here, we examine primary cilia length with exposure to the quintessential inflammatory cytokine interleukin-1 (IL-1), which is up-regulated in OA. We then test the hypothesis that the cilium is involved in mediating the downstream inflammatory response. Primary chondrocytes treated with IL-1 exhibited a 50 % increase in cilia length after 3 h exposure. IL-1-induced cilia elongation was also observed in human fibroblasts. In chondrocytes, this elongation occurred via a protein kinase A (PKA)-dependent mechanism. G-protein coupled adenylate cyclase also regulated the length of chondrocyte primary cilia but not downstream of IL-1. Chondrocytes treated with IL-1 exhibit a characteristic increase in the release of the inflammatory chemokines, nitric oxide and prostaglandin E2. However, in cells with a mutation in IFT88 whereby the cilia structure is lost, this response to IL-1 was significantly attenuated and, in the case of nitric oxide, completely abolished. Inhibition of IL-1-induced cilia elongation by PKA inhibition also attenuated the chemokine response. These results suggest that cilia assembly regulates the response to inflammatory cytokines. Therefore, the cilia proteome may provide a novel therapeutic target for the treatment of inflammatory pathologies, including OA

Modeling the resonant planetary system GJ876

The two planets about the star GJ 876 appear to have undergone extensive migration from their point of origin in the protoplanetary disk -- both because of their close proximity to the star (30 and 60 day orbital periods) and because of their occupying three stable orbital resonances at the 2:1 mean-motion commensurability. The resonances were most likely established by converging differential migration of the planets leading to capture into the resonances. A problem with this scenario is that continued migration of the system while it is trapped in the resonances leads to orbital eccentricities that rapidly exceed the observational upper limits of e_1 = 0.31 and e_2 = 0.05. As seen in forced 3-body simulations, lower eccentricities would persist during migration only for an applied eccentricity damping. Here we explore the evolution of the GJ 876 system using two-dimensional hydrodynamical simulations that include viscous heating and radiative effects. We find that a hydrodynamic evolution within the resonance, where only the outer planet interacts with the disk, always rapidly leads to large values of eccentricities that exceed those observed. Only if mass is removed from the disk on a time scale of the order of the migration time scale (before there has been extensive migration after capture), as might occur for photoevaporation in the late phases of planet formation, can we end up with eccentricities that are consistent with the observations.Comment: Paper accepted by A&A, 17 Pages, 17 Figure

Discovery and characterization of WASP-6b, an inflated sub-Jupiter mass planet transiting a solar-type star

We report the discovery of WASP-6b, an inflated sub-Jupiter mass planet transiting every 3.3610060^{\rm + 0.0000022 }_ days a mildly metal-poor solar-type star of magnitude V = 11.9. A combined analysis of the WASP photometry, high-precision followup transit photometry and radial velocities yield a planetary mass M_{\rm p} = 0.503^_ $M_{\rm J}$ and radius R_{\rm p} = 1.224^_ $R_{\rm J}$, resulting in a density $\rho_{\rm p} = 0.27 \pm 0.05$ $\rho_{\rm J}$. The mass and radius for the host star are M_\ast = 0.88^_ $M_\odot$ and R_\ast = 0.870^_ $R_\odot$. The non-zero orbital eccentricity e = 0.054^{\rm +0.018}_ that we measure suggests that the planet underwent a massive tidal heating ~1 Gyr ago that could have contributed to its inflated radius. High-precision radial velocities obtained during a transit allow us to measure a sky-projected angle between the stellar spin and orbital axis \beta = 11^_ deg. In addition to similar published measurements, this result favors a dominant migration mechanism based on tidal interactions with a protoplanetary disk

Defending the genome from the enemy within:mechanisms of retrotransposon suppression in the mouse germline

The viability of any species requires that the genome is kept stable as it is transmitted from generation to generation by the germ cells. One of the challenges to transgenerational genome stability is the potential mutagenic activity of transposable genetic elements, particularly retrotransposons. There are many different types of retrotransposon in mammalian genomes, and these target different points in germline development to amplify and integrate into new genomic locations. Germ cells, and their pluripotent developmental precursors, have evolved a variety of genome defence mechanisms that suppress retrotransposon activity and maintain genome stability across the generations. Here, we review recent advances in understanding how retrotransposon activity is suppressed in the mammalian germline, how genes involved in germline genome defence mechanisms are regulated, and the consequences of mutating these genome defence genes for the developing germline

Uncanny Objects and the Fear of the Familiar:Hiding from Akan Witches in New York City

This article examines the cosmology and secret practices of West African traditional priests in New York City in preventing the spread of witchcraft, an evil invisible spirit transmitted between female members of the Akan matrilineage. Explored is an uncanny dynamic as everyday habitus becomes increasingly strange in the world of a young Ghanaian woman in the Bronx, who has become petrified of insinuations of witchcraft from close family members. In trying to hide the young woman from infection by her fellow witches, Akan priests attempt to ‘capture’ her habits and everyday routines, calling upon the iconic magic of New York City in order to ‘misplace’ familiarity within the anonymity of Manhattan. In this process, the transmission of the witch’s spirit to the intended victim is disturbed as the victim’s life and things are moved. Nowhere to be found, the witch shifts her attention to other victims