Differential Proteomic Analysis of Human Saliva using Tandem Mass Tags Quantification for Gastric Cancer Detection.

Novel biomarkers and non-invasive diagnostic methods are urgently needed for the screening of gastric cancer to reduce its high mortality. We employed quantitative proteomics approach to develop discriminatory biomarker signatures from human saliva for the detection of gastric cancer. Salivary proteins were analyzed and compared between gastric cancer patients and matched control subjects by using tandem mass tags (TMT) technology. More than 500 proteins were identified with quantification, and 48 of them showed significant difference expression (p < 0.05) between normal controls and gastric cancer patients, including 7 up-regulated proteins and 41 down-regulated proteins. Five proteins were selected for initial verification by ELISA and three were successfully verified, namely cystatin B (CSTB), triosephosphate isomerase (TPI1), and deleted in malignant brain tumors 1 protein (DMBT1). All three proteins could differentiate gastric cancer patients from normal control subjects, dramatically (p < 0.05). The combination of these three biomarkers could reach 85% sensitivity and 80% specificity for the detection of gastric cancer with accuracy of 0.93. This study provides the proof of concept of salivary biomarkers for the non-invasive detection of gastric cancer. It is highly encouraging to turn these biomarkers into an applicable clinical test after large scale validation

microRNA-33a-5p increases radiosensitivity by inhibiting glycolysis in melanoma.

Glycolysis was reported to have a positive correlation with radioresistance. Our previous study found that the miR-33a functioned as a tumor suppressor in malignant melanoma by targeting hypoxia-inducible factor1-alpha (HIF-1α), a gene known to promote glycolysis. However, the role of miR-33a-5p in radiosensitivity remains to be elucidated. We found that miR-33a-5p was downregulated in melanoma tissues and cells. Cell proliferation was downregulated after overexpression of miR-33a-5p in WM451 cells, accompanied by a decreased level of glycolysis. In contrast, cell proliferation was upregulated after inhibition of miR-33a-5p in WM35 cells, accompanied by increased glycolysis. Overexpression of miR-33a-5p enhanced the sensitivity of melanoma cells to X-radiation by MTT assay, while downregulation of miR-33a-5p had the opposite effects. Finally, in vivo experiments with xenografts in nude mice confirmed that high expression of miR-33a-5p in tumor cells increased radiosensitivity via inhibiting glycolysis. In conclusions, miR-33a-5p promotes radiosensitivity by negatively regulating glycolysis in melanoma

Experimental unconditionally secure bit commitment

Bit commitment is a fundamental cryptographic task that guarantees a secure commitment between two mutually mistrustful parties and is a building block for many cryptographic primitives, including coin tossing, zero-knowledge proofs, oblivious transfer and secure two-party computation. Unconditionally secure bit commitment was thought to be impossible until recent theoretical protocols that combine quantum mechanics and relativity were shown to elude previous impossibility proofs. Here we implement such a bit commitment protocol. In the experiment, the committer performs quantum measurements using two quantum key distribution systems and the results are transmitted via free-space optical communication to two agents separated with more than 20 km. The security of the protocol relies on the properties of quantum information and relativity theory. We show that, in each run of the experiment, a bit is successfully committed with less than 5.68*10^-2 cheating probability. Our result demonstrates unconditionally secure bit commitment and the experimental feasibility of relativistic quantum communication.Comment: 15 pages, 2 figure

The Different Nature in Seyfert 2 Galaxies With and Without Hidden Broad-Line Regions

We compile a large sample of 120 Seyfert 2 galaxies (Sy2s) which contains 49 hidden broad-line region (HBLR) Sy2s and 71 non-HBLR Sy2s. From the difference in the power sources between two groups, we test if HBLR Sy2s are dominated by active galactic nuclei (AGNs), and if non-HBLR Sy2s are dominated by starbursts. We show that: (1) HBLR Sy2s have larger accretion rates than non-HBLR Sy2s; (2) HBLR Sy2s have larger \Nev $\lambda 14.32$/\Neii $\lambda 12.81$ and \oiv $\lambda 25.89$/\Neii $\lambda 12.81$ line ratios than non-HBLR Sy2s; (3) HBLR Sy2s have smaller $IRAS$ $f_{60}/f_{25}$ flux ratio which shows the relative strength of the host galaxy and nuclear emission than non-HBLR Sy2s. So we suggest that HBLR Sy2s and non-HBLR Sy2s are AGN-dominated and starburst-dominated, respectively. In addition, non-HBLR Sy2s can be classified into the luminous ($L_{\rm [O III]}>10^{41} \rm ergs s^{-1}$) and less luminous ($L_{\rm [O III]}<10^{41} \rm ergs s^{-1}$) samples, when considering only their obscuration. We suggest that: (1) the invisibility of polarized broad lines (PBLs) in the luminous non-HBLR Sy2s depends on the obscuration; (2) the invisibility of PBLs in the less luminous non-HBLR Sy2s depends on the very low Eddington ratio rather than the obscuration.Comment: Accepted by ApJ, 11 pages, 4 figure