Mechanism of pulse magneto-oscillation grain refinement on pure Al

Pulse magneto-oscillation (PMO) was developed as a novel technique to refine the solidification structure of pure aluminium. Its grain refining mechanism was proposed. The PMO refinement mechanism is that the nucleus falls off from the mould wall and drifts into the melt under the action of PMO. The solidification structure of Al melt depends on the linear electric current density, and also the discharge and oscillation frequencies. The radial pressure of PMO sound wave is the major factor that contributes to the migration of nucleus into the melt

Research on the Current Situation and Guiding Path of College Students’ Participation in Charitable Activities

As the traditional virtue of the Chinese nation, the excellent quality of helping others is an important support for building a harmonious socialist society. Charity activities are an important form to carry forward its excellent quality. College students as the future hope of the motherland, by encouraging and guiding college students to participate in charitable activities to cultivate relevant excellent quality is the important content of shaping contemporary higher education talents and realizing their personal life value, but at present, there are still problems in college students’ participation in charitable activities that need to be solved urgently. Taking YuLin University as an example, this paper points out the relevant theoretical concepts, analyzes the significance of college students’ participation in public welfare and charitable activities. Through data investigation, it is concluded that there are some problems in YuLin University students’ participation in public welfare and charitable activities, such as less participation, impure participation motivation, single participation information acquisition channel and distrust of relevant groups. This paper puts forward the improvement path from the aspects of individual, family, school and society in order to play an optimization role

A Common Variant in CLDN14 is Associated with Primary Biliary Cirrhosis and Bone Mineral Density.

Primary biliary cirrhosis (PBC), a chronic autoimmune liver disease, has been associated with increased incidence of osteoporosis. Intriguingly, two PBC susceptibility loci identified through genome-wide association studies are also involved in bone mineral density (BMD). These observations led us to investigate the genetic variants shared between PBC and BMD. We evaluated 72 genome-wide significant BMD SNPs for association with PBC using two European GWAS data sets (n = 8392), with replication of significant findings in a Chinese cohort (685 cases, 1152 controls). Our analysis identified a novel variant in the intron of the CLDN14 gene (rs170183, Pfdr = 0.015) after multiple testing correction. The three associated variants were followed-up in the Chinese cohort; one SNP rs170183 demonstrated consistent evidence of association in diverse ethnic populations (Pcombined = 2.43 × 10(-5)). Notably, expression quantitative trait loci (eQTL) data revealed that rs170183 was correlated with a decline in CLDN14 expression in both lymphoblastoid cell lines and T cells (Padj = 0.003 and 0.016, respectively). In conclusion, our study identified a novel PBC susceptibility variant that has been shown to be strongly associated with BMD, highlighting the potential of pleiotropy to improve gene discovery

A novel ZRS variant causes preaxial polydactyly type I by increased sonic hedgehog expression in the developing limb bud.

PurposePreaxial polydactyly (PPD) is a common congenital hand malformation classified into four subtypes (PPD I-IV). Variants in the zone of polarizing activity regulatory sequence (ZRS) within intron 5 of the LMBR1 gene are linked to most PPD types. However, the genes responsible for PPD I and the underlying mechanisms are unknown.MethodsA rare large four-generation family with isolated PPD I was subjected to genome-wide genotyping and sequence analysis. In vitro and in vivo functional studies were performed in Caco-2 cells, 293T cells, and a knockin transgenic mouse model.ResultsA novel g.101779T>A (reference sequence: NG_009240.2; position 446 of the ZRS) variant segregates with all PPD I-affected individuals. The knockin mouse with this ZRS variant exhibited PPD I phenotype accompanying ectopic and excess expression of Shh. We confirmed that HnRNP K can bind the ZRS and SHH promoters. The ZRS mutant enhanced the binding affinity for HnRNP K and upregulated SHH expression.ConclusionOur results identify the first PPD I disease-causing variant. The variant leading to PPD I may be associated with enhancing SHH expression mediated by HnRNP K. This study adds to the ZRS-associated syndromes classification system for PPD and clarifies the underlying molecular mechanisms

Genome-Wide Identification and Salt Stress Response Analysis of the bZIP Transcription Factor Family in Sugar Beet

As one of the largest transcription factor families in plants, bZIP transcription factors play important regulatory roles in different biological processes, especially in the process of stress response. Salt stress inhibits the growth and yield of sugar beet. However, bZIP-related studies in sugar beet (Beta vulgaris L.) have not been reported. This study aimed to identify the bZIP transcription factors in sugar beet and analyze their biological functions and response patterns to salt stress. Using bioinformatics, 48 BvbZIP genes were identified in the genome of sugar beet, encoding 77 proteins with large structural differences. Collinearity analysis showed that three pairs of BvbZIP genes were fragment replication genes. The BvbZIP genes were grouped according to the phylogenetic tree topology and conserved structures, and the results are consistent with those reported in Arabidopsis. Under salt stress, the expression levels of most BvbZIP genes were decreased, and only eight genes were up-regulated. GO analysis showed that the BvbZIP genes were mainly negatively regulated in stress response. Protein interaction prediction showed that the BvbZIP genes were mainly involved in light signaling and ABA signal transduction, and also played a certain role in stress responses. In this study, the structures and biological functions of the BvbZIP genes were analyzed to provide foundational data for further mechanistic studies and for facilitating the efforts toward the molecular breeding of stress-resilient sugar beet

GWAS Identifies Novel Susceptibility Loci on 6p21.32 and 21q21.3 for Hepatocellular Carcinoma in Chronic Hepatitis B Virus Carriers

Genome-wide association studies (GWAS) have recently identified KIF1B as susceptibility locus for hepatitis B virus (HBV)–related hepatocellular carcinoma (HCC). To further identify novel susceptibility loci associated with HBV–related HCC and replicate the previously reported association, we performed a large three-stage GWAS in the Han Chinese population. 523,663 autosomal SNPs in 1,538 HBV–positive HCC patients and 1,465 chronic HBV carriers were genotyped for the discovery stage. Top candidate SNPs were genotyped in the initial validation samples of 2,112 HBV–positive HCC cases and 2,208 HBV carriers and then in the second validation samples of 1,021 cases and 1,491 HBV carriers. We discovered two novel associations at rs9272105 (HLA-DQA1/DRB1) on 6p21.32 (OR = 1.30, P = 1.13×$10^{−19}$) and rs455804 (GRIK1) on 21q21.3 (OR = 0.84, P = 1.86×$10^{−8}$), which were further replicated in the fourth independent sample of 1,298 cases and 1,026 controls (rs9272105: OR = 1.25, P = 1.71×$10^{−4}$; rs455804: OR = 0.84, P = 6.92×$10^{−3}$). We also revealed the associations of HLA-DRB1*0405 and 0901*0602, which could partially account for the association at rs9272105. The association at rs455804 implicates GRIK1 as a novel susceptibility gene for HBV–related HCC, suggesting the involvement of glutamate signaling in the development of HBV–related HCC

Design and analysis of longitudinal-flexural hybrid transducer for ultrasonic peen forming

Ultrasonic peen forming (UPF) is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming methods. Although ultrasonic transducers with longitudinal vibration have been widely studied, they have seldom been incorporated into UPF devices for machining in confined spaces. To meet the requirements of this type of machining, a sandwich-type piezoelectric transducer with coupled longitudinal–flexural vibrational modes is proposed. The basic structure of the transducer is designed to obtain large vibrational amplitudes in both modes. Experimental results obtained with a prototype device demonstrate the feasibility of the proposed transducer. The measured vibrational amplitude for the working face in the longitudinal vibrational mode is 1.0 μm, and electrical matching increases this amplitude by 40%. The flexural vibration characteristics of the same prototype transducer are also tested and are found to be slightly smaller than those of longitudinal mode. The resultant working strokes of the UPF impact pins reach 1.7 mm and 1.2 mm in the longitudinal and flexural modes, respectively. The forming capability of the prototype has been evaluated via 15-min machining on standard 2024-T351 aluminum plates. After UPF, an improved surface morphology with lower surface roughness is obtained. The aluminum plate test piece has an apparent upper deformation with an arc height of 0.64 mm. The measured peak value of the compressive residual stress is around 250 MPa, appearing at a depth of 100 μm. The proposed longitudinal–flexural hybrid transducer thus provides a high-performance tool for plate peen forming in confined spaces

Master-Slave Synchronization of Stochastic Neural Networks with Mixed Time-Varying Delays

This paper investigates the problem on master-salve synchronization for stochastic neural networks with both time-varying and distributed time-varying delays. Together with the drive-response concept, LMI approach, and generalized convex combination, one novel synchronization criterion is obtained in terms of LMIs and the condition heavily depends on the upper and lower bounds of state delay and distributed one. Moreover, the addressed systems can include some famous network models as its special cases, which means that our methods extend those present ones. Finally, two numerical examples are given to demonstrate the effectiveness of the presented scheme

Attitudes toward statistics in medical postgraduates: measuring, evaluating and monitoring

Abstract Background In medical training, statistics is considered a very difficult course to learn and teach. Current studies have found that students’ attitudes toward statistics can influence their learning process. Measuring, evaluating and monitoring the changes of students’ attitudes toward statistics are important. Few studies have focused on the attitudes of postgraduates, especially medical postgraduates. Our purpose was to understand current attitudes regarding statistics held by medical postgraduates and explore their effects on students’ achievement. We also wanted to explore the influencing factors and the sources of these attitudes and monitor their changes after a systematic statistics course. Methods A total of 539 medical postgraduates enrolled in a systematic statistics course completed the pre-form of the Survey of Attitudes Toward Statistics −28 scale, and 83 postgraduates were selected randomly from among them to complete the post-form scale after the course. Results Most medical postgraduates held positive attitudes toward statistics, but they thought statistics was a very difficult subject. The attitudes mainly came from experiences in a former statistical or mathematical class. Age, level of statistical education, research experience, specialty and mathematics basis may influence postgraduate attitudes toward statistics. There were significant positive correlations between course achievement and attitudes toward statistics. In general, student attitudes showed negative changes after completing a statistics course. Conclusions The importance of student attitudes toward statistics must be recognized in medical postgraduate training. To make sure all students have a positive learning environment, statistics teachers should measure their students’ attitudes and monitor their change of status during a course. Some necessary assistance should be offered for those students who develop negative attitudes. </jats:sec