Phytochemical analyses of Ziziphus jujuba Mill. var. spinosa seed by ultrahigh performance liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry

Analyst138226881-688

Enhanced DPPH radical scavenging activity and DNA protection effect of litchi pericarp extract by Aspergillus awamori bioconversion

BACKGROUND: Litchi (Litchi chinensis Sonn.) pericarp is a major byproduct which contains a significant amount of polyphenol. This study was designed to biotransformation litchi pericarp extract (LPE) by Aspergillus awamori to produce more bioactive compounds with stronger antioxidant activities. RESULTS: The study exhibited that the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities significantly (p < 0.05) increased from 15.53% to 18.23% in the water-extracted fraction and from 25.41% to 36.82% in the ethyl acetate-extracted fraction. Application of DNA cleavage assay further demonstrated the enhanced protection effect of the fermented phenolics on DNA damage. It is also noted that the water-extracted fraction of the fermented LPE possessed a much stronger capacity than the ethyl acetate-extracted fraction to prevent from damage of supercoiled DNA. Interestingly, it was found that some new compounds such as catechin and quercetin appeared after of A. awamori fermentation of LPE, which could account for the enhanced antioxidant activity. CONCLUSION: The DPPH radical scavenging activity and DNA protection effect of LPE were increased by Aspergillus awamori bioconversion while some compounds responsible for the enhanced antioxidant activity were identified. This study provided an effective way of utilizing fruit pericarp as a readily accessible source of the natural antioxidants in food industry and, thus, extended the application area such as fruit by-products

An exotic fruit with high nutritional value: Kadsura coccinea fruit

This research was to determine nutritional composition, essential and toxic elemental content, and major phenolic acid with antioxidant activity in Kadsura coccinea fruit. The results indicated that Kadsura coccinea fruit exhibited the high contents of total protein, total fat, ash and essential elements such as calcium (Ca), ferrum (Fe) and phosphorus (P). The levels of four common toxic elements, i.e. cadmium (Cd), mercury (Hg), arsenic (As) and lead (Pb), were lower than legal limits. By high-performance liquid chromatography (HPLC) analysis, gallic acid was identified as major phenolic acid in peel and pulp tissues. Its contents were no significant difference in both tissues. In comparison with two commercial antioxidants, the major phenolic acid extracted from Kadsura coccinea exhibited stronger 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity and reducing power. Kadsura coccinea fruit is a good source of nutrition and natural antioxidant. It is worthwhile to popularize this exotic fruit around the world

COMPARISON OF BRAIN METABOLITE CHANGES IN MANGANESE-EXPOSED WELDERS AND SMELTERS

poster abstractExcessive manganese (Mn) exposure is known to cause cognitive, psychiatric and motor deficits. Mn overexposure occurs in different occupational settings, where the type and level of exposure may vary. Magnetic resonance imaging (MRI) and spectroscopy (MRS) can be used to evaluate brain Mn accumulation and to measure Mn-induced metabolite changes non-invasively. The aim of this study was to compare metabolite changes among different brain regions of welders and smelters following occupational Mn exposure. Nine Mn-exposed smelters, 14 Mn-exposed welders and 23 male matched controls were recruited from a cohort of workers from two factories in China (mean airborne Mn level: 0.227 and 0.025 mg/m3 for smelters and welders, respectively). Short-echo-time 1H MRS spectra were acquired in each subject from four volumes of interest: the frontal cortex, posterior cingulate cortex, hippocampus, and thalamus. We found that 1) in the frontal cortex, significantly decreased creatine (Cr), glutamate (Glu) and glutathione (GSH) were found in welders, whereas decreased Glu was found in smelters as compared to controls. 2) In the thalamus, reduced myo-inositol was found in both smelters and welders, while Glu and GSH were decreased in welders. These results suggest that Mn-induced brain metabolite changes may be regional in nature and more extensive in welders than in smelters. The frontal cortex seems to show a more profound change than the other brain areas tested among Mn exposed subjects. Further studies are needed to investigate the effects of exposure type and length on the mechanism of Mn neurotoxicity. (Supported by NIH/NIEHS R21 ES-017498, National Science Foundation of China Grant #81072320 and 30760210)

Design of a low voltage pipeline ADC with digital calibration and a bandgap reference circuit

The advances of sub-micron integrated circuit (IC) technology demands reduction of supply voltage due to reliability issues and power dissipation considerations. However, conventional analog circuits have difficulty to function when supply voltage is less than 1.5 V. Emphasis of this work was placed on the circuit architecture at low supply voltage where conventional switched-capacitor circuits cannot work. A 1 V, 12-bit, 5MS/s pipeline analog-to-digital converter was implemented in TSMC 0.25 um CMOS technology. Biased inverting op amp was used to realize sample-and-hold and sample-and-amplify without critical MOS switches. Current mode approaches were used in comparator and common-mode feedback circuit to successfully replace traditional voltage input approaches. High accuracy was achieved by 1.5-bit per stage architecture and the use of digital calibration, which have been demonstrated the capability to linearize the system in spite of the presence of large comparator offsets, stage gain errors and non-ideal reference voltage levels. An on chip low voltage bandgap reference using transimpedance amplifier was also implemented in a 1.2 um CMOS. The variations of the output voltage over temperature (O 0C [Less than or equal to] T [Less than or equal to] 100 0C) were measured to be less than ±l % without resistor trimming

RS-Corrector: Correcting the Racial Stereotypes in Latent Diffusion Models

Recent text-conditioned image generation models have demonstrated an exceptional capacity to produce diverse and creative imagery with high visual quality. However, when pre-trained on billion-sized datasets randomly collected from the Internet, where potential biased human preferences exist, these models tend to produce images with common and recurring stereotypes, particularly for certain racial groups. In this paper, we conduct an initial analysis of the publicly available Stable Diffusion model and its derivatives, highlighting the presence of racial stereotypes. These models often generate distorted or biased images for certain racial groups, emphasizing stereotypical characteristics. To address these issues, we propose a framework called "RS-Corrector", designed to establish an anti-stereotypical preference in the latent space and update the latent code for refined generated results. The correction process occurs during the inference stage without requiring fine-tuning of the original model. Extensive empirical evaluations demonstrate that the introduced \themodel effectively corrects the racial stereotypes of the well-trained Stable Diffusion model while leaving the original model unchanged.Comment: 16 pages, 15 figures, conferenc

RoDyn-SLAM: Robust Dynamic Dense RGB-D SLAM with Neural Radiance Fields

Leveraging neural implicit representation to conduct dense RGB-D SLAM has been studied in recent years. However, this approach relies on a static environment assumption and does not work robustly within a dynamic environment due to the inconsistent observation of geometry and photometry. To address the challenges presented in dynamic environments, we propose a novel dynamic SLAM framework with neural radiance field. Specifically, we introduce a motion mask generation method to filter out the invalid sampled rays. This design effectively fuses the optical flow mask and semantic mask to enhance the precision of motion mask. To further improve the accuracy of pose estimation, we have designed a divide-and-conquer pose optimization algorithm that distinguishes between keyframes and non-keyframes. The proposed edge warp loss can effectively enhance the geometry constraints between adjacent frames. Extensive experiments are conducted on the two challenging datasets, and the results show that RoDyn-SLAM achieves state-of-the-art performance among recent neural RGB-D methods in both accuracy and robustness.Comment: IEEE RAL 202

Anoxia Treatment for Delaying Skin Browning, Inhibiting Disease Development and Maintaining the Quality of Litchi Fruit

Litchi fruit has a very short shelf life after harvest, so marketers and consumers alike desire longer periods of storage, transportation and distribution. To extend shelf life, anoxia treatments were used for the fruit. Litchi fruit were exposed to pure N2 for 0, 3, 6, 12 or 24 h. They were then kept individually in closed but vented containers for 6 days in the dark at 20 °C and 95–100 % relative humidity. Exposure of litchi fruit to N2 for 3 or 6 h markedly delayed skin browning, reduced rot development and maintained higher concentrations of total soluble solids, titratable acidity and ascorbic acid after 6 days of storage. Anoxia treatment for 24 h reduced browning index, but it accelerated disease development, compared to the control. Thus, a pre-storage pure N2 treatment for 3 or 6 h can be an effective means of reducing rotting while maintaining the physical quality of the fruit

Effects of Hot Water Immersion on Storage Quality of Fresh Broccoli Heads

Freshly harvested broccoli heads were immersed for 0, 1, 4 or 8 min into hot water at 45 °C, and then were hydrocooled rapidly for 10 min at 10 °C. Following these treatments, the broccoli were air-dried for 30 min, then packed in commercial polymeric film bags, and, finally, stored for 16 days at –1, 1, and 12 °C. The samples treated with hot water maintained high contents of chlorophyll concentrations, their yellowing rate was delayed, and fungal infection and chilling or freezing injury were inhibited markedly. Compared to non-heat-treated broccoli, a lower level of peroxidase activity with a relatively higher chlorophyll concentration was observed when broccoli were treated with hot water. Among these heat treatments, immersion in hot water for 4 min at 45 °C was the most effective for maintaining the quality of harvested broccoli heads