Fluorescent Probes Design Strategies for Imaging Mitochondria and Lysosomes

Modern cellular biology faces several major obstacles, such as the determination of the concentration of active sites corresponding to chemical substances. In recent years, the popular small-molecule fluorescent probes have completely changed the understanding of cellular biology through their high sensitivity toward specific substances in various organisms. Mitochondria and lysosomes are significant organelles in various organisms, and their interaction is closely related to the development of various diseases. The investigation of their structure and function has gathered tremendous attention from biologists. The advanced nanoscopic technologies have replaced the diffraction-limited conventional imaging techniques and have been developed to explore the unknown aspects of mitochondria and lysosomes with a sub-diffraction resolution. Recent progress in this field has yielded several excellent mitochondria- and lysosome-targeted fluorescent probes, some of which have demonstrated significant biological applications. Herein, we review studies that have been carried out to date and suggest future research directions that will harness the considerable potential of mitochondria- and lysosome-targeted fluorescent probes

The impact of a PERMA model-based positive psychology intervention on fear of stroke recurrence: a randomized controlled trial

IntroductionThis study aimed to examine the effects of a positive psychological intervention, grounded in the PERMA model, on fear levels, psychological capital, overall well-being, and quality of life among stroke patients.MethodsA single-blind, two-arm randomized controlled trial with a repeated measures design was conducted at the Affiliated Hospital of Jiangnan University from January to December 2023. A total of 125 patients experiencing fear of stroke recurrence were randomly assigned to either the intervention group (n = 63), which received a positive psychological intervention based on the PERMA model, or the control group (n = 62), which received standard care. We assessed fear levels, psychological capital, well-being, and quality of life at baseline (T0), on the day of discharge (T1), 2 weeks post-discharge (T2), and 4 weeks post-discharge (T3). The scores of the two groups were compared post-intervention using the Generalized Estimation Equation (GEE) model to analyze the effects of time, group membership, and their interaction.ResultsThe intervention group showed statistically significant improvements compared to the control group, including reduced fear levels (T2: t = −2.094, p = 0.038; T3: t = −2.207, p = 0.029), increased psychological capital (T2: t = 2.053, p = 0.042; T3: t = 2.820, p = 0.006), enhanced well-being (T2: t = 2.037, p = 0.044; T3: t = 2.761, p = 0.007), and better quality of life (T2: t = 2.083, p = 0.039; T3: t = 2.453, p = 0.016) at both T2 and T3. Additionally, significant time-related changes were observed in fear levels, psychological capital, well-being, and quality of life (χ2 = 45.275, p < 0.001; χ2 = 37.848, p < 0.001; χ2 = 48.255, p < 0.001; χ2 = 34.231, p < 0.001, respectively). Notably, the interaction effects were statistically significant for well-being (p < 0.05).DiscussionThe PERMA-based intervention had a short-term positive effect, reducing fear levels while enhancing psychological capital, well-being, and quality of life among stroke patients.Clinical Trial Registrationhttps://www.chictr.org.cn/showproj.html?proj=230313