A Qualitative Examination of Graduate Advising Relationships: The Advisor Perspective

Nineteen counseling psychology faculty members were interviewed regarding their advising relationships with doctoral students. Advisors informally learned to advise from their experiences with their advisor and their advisees and defined their role as supporting and advocating for advisees as they navigated their doctoral program. Advisors identified personal satisfaction as a benefit and time demands as a cost of advising. Good advising relationships were facilitated by advisees’ positive personal or professional characteristics, mutual respect, open communication, similarity in career path between advisor and advisee, and lack of conflict. Difficult relationships were affected by advisees’ negative personal or professional characteristics, lack of respect, research struggles, communication problems, advisors feeling ineffective working with advisees, disruption or rupture of the relationship, and conflict avoidance. Implications for research and training are discussed

Upscaling fluxes from towers to regions, continents and global scales using datadriven approaches

Quantifying the current carbon cycle of terrestrial ecosystems requires that we translate spatially sparse measurements into consistent, gridded flux estimates at the regional scale. This is particularly challenging in heterogeneous regions such as the northern forests of the United States. We use a network of 17 eddy covariance flux towers deployed across the Upper Midwest region of northern Wisconsin and Michigan and upscale flux observations from towers to the regional scale. This region is densely instrumented and provides a unique test bed for regional upscaling. We develop a simple Diagnostic Carbon Flux Model (DCFM) and use flux observations and a data assimilation approach to estimate the model parameters. We then use the optimized model to produce gridded flux estimates across the region. We find that model parameters vary not only across plant functional types (PFT) but also within a given PFT. Our results show that the parameter estimates from a single site are not representative of the parameter values of a given PFT; cross-site (or joint) optimization using observations from multiple sites encompassing a range of site and climate conditions considerably improves the representativeness and robustness of parameter estimates. Parameter variability within a PFT can result in substantial variability in regional flux estimates. We also find that land cover representation including land cover heterogeneity and the spatial resolution and accuracy of land cover maps can lead to considerable uncertainty in regional flux estimates. In heterogeneous, complex regions, detailed and accurate land cover maps are essential for accurate estimation of regional fluxes

Upscaling carbon fluxes from towers to the regional scale: Influence of parameter variability and land cover representation on regional flux estimates

Quantifying the current carbon cycle of terrestrial ecosystems requires that we translate spatially sparse measurements into consistent, gridded flux estimates at the regional scale. This is particularly challenging in heterogeneous regions such as the northern forests of the United States. We use a network of 17 eddy covariance flux towers deployed across the Upper Midwest region of northern Wisconsin and Michigan and upscale flux observations from towers to the regional scale. This region is densely instrumented and provides a unique test bed for regional upscaling. We develop a simple Diagnostic Carbon Flux Model (DCFM) and use flux observations and a data assimilation approach to estimate the model parameters. We then use the optimized model to produce gridded flux estimates across the region. We find that model parameters vary not only across plant functional types (PFT) but also within a given PFT. Our results show that the parameter estimates from a single site are not representative of the parameter values of a given PFT; cross-site (or joint) optimization using observations from multiple sites encompassing a range of site and climate conditions considerably improves the representativeness and robustness of parameter estimates. Parameter variability within a PFT can result in substantial variability in regional flux estimates. We also find that land cover representation including land cover heterogeneity and the spatial resolution and accuracy of land cover maps can lead to considerable uncertainty in regional flux estimates. In heterogeneous, complex regions, detailed and accurate land cover maps are essential for accurate estimation of regional fluxes

Interfacing peripheral nerve with macro-sieve electrodes following spinal cord injury

Macro-sieve electrodes were implanted in the sciatic nerve of five adult male Lewis rats following spinal cord injury to assess the ability of the macro-sieve electrode to interface regenerated peripheral nerve fibers post-spinal cord injury. Each spinal cord injury was performed via right lateral hemisection of the cord at the T9–10 site. Five months post-implantation, the ability of the macro-sieve electrode to interface the regenerated nerve was assessed by stimulating through the macro-sieve electrode and recording both electromyography signals and evoked muscle force from distal musculature. Electromyography measurements were recorded from the tibialis anterior and gastrocnemius muscles, while evoked muscle force measurements were recorded from the tibialis anterior, extensor digitorum longus, and gastrocnemius muscles. The macro-sieve electrode and regenerated sciatic nerve were then explanted for histological evaluation. Successful sciatic nerve regeneration across the macro-sieve electrode interface following spinal cord injury was seen in all five animals. Recorded electromyography signals and muscle force recordings obtained through macro-sieve electrode stimulation confirm the ability of the macro-sieve electrode to successfully recruit distal musculature in this injury model. Taken together, these results demonstrate the macro-sieve electrode as a viable interface for peripheral nerve stimulation in the context of spinal cord injury

Can one hear the shape of a population history?

Reconstructing past population size from present day genetic data is a major goal of population genetics. Recent empirical studies infer population size history using coalescent-based models applied to a small number of individuals. Here we provide tight bounds on the amount of exact coalescence time data needed to recover the population size history of a single, panmictic population at a certain level of accuracy. In practice, coalescence times are estimated from sequence data and so our lower bounds should be taken as rather conservative.Comment: 22 pages, 7 figures; v2 is significantly revised from v

Novel tumor suppressor microRNA at frequently deleted chromosomal region 8p21 regulates epidermal growth factor receptor in prostate cancer.

Genomic loss of chromosome (chr) 8p21 region, containing prostate-specific NKX3.1 gene, is a frequent alteration of the prostate cancer (PCa) oncogenome. We propose a novel, paradigm shifting hypothesis that this frequently deleted locus is also associated with a cluster of microRNA genes- miR-3622a/b- that are lost in PCa and play an important mechanistic role in progression and metastasis. In this study, we demonstrate the role of miR-3622b in prostate cancer. Expression analyses in a cohort of PCa clinical specimens and cell lines show that miR-3622b expression is frequently lost in prostate cancer. Low miR-3622b expression was found to be associated with tumor progression and poor biochemical recurrence-free survival. Further, our analyses suggest that miR-3622b expression is a promising prostate cancer diagnostic biomarker that exhibits 100% specificity and 66% sensitivity. Restoration of miR-3622b expression in PCa cell lines led to reduced cellular viability, proliferation, invasiveness, migration and increased apoptosis. miR-3622b overexpression in vivo induced regression of established prostate tumor xenografts pointing to its therapeutic potential. Further, we found that miR-3622b directly represses Epidermal Growth Factor Receptor (EGFR). In conclusion, our study suggests that miR-3622b plays a tumor suppressive role and is frequently downregulated in prostate cancer, leading to EGFR upregulation. Importantly, miR-3622b has associated diagnostic, prognostic and therapeutic potential. Considering the association of chr8p21 loss with poor prognosis, our findings are highly significant and support a novel concept that associates a long standing observation of frequent loss of a chromosomal region with a novel miRNA in prostate cancer

Functional Evolution of a cis-Regulatory Module

Lack of knowledge about how regulatory regions evolve in relation to their structure–function may limit the utility of comparative sequence analysis in deciphering cis-regulatory sequences. To address this we applied reverse genetics to carry out a functional genetic complementation analysis of a eukaryotic cis-regulatory module—the even-skipped stripe 2 enhancer—from four Drosophila species. The evolution of this enhancer is non-clock-like, with important functional differences between closely related species and functional convergence between distantly related species. Functional divergence is attributable to differences in activation levels rather than spatiotemporal control of gene expression. Our findings have implications for understanding enhancer structure–function, mechanisms of speciation and computational identification of regulatory modules