Compactness Determines the Success of Cube and Octahedron Self-Assembly

Nature utilizes self-assembly to fabricate structures on length scales ranging from the atomic to the macro scale. Self-assembly has emerged as a paradigm in engineering that enables the highly parallel fabrication of complex, and often three-dimensional, structures from basic building blocks. Although there have been several demonstrations of this self-assembly fabrication process, rules that govern a priori design, yield and defect tolerance remain unknown. In this paper, we have designed the first model experimental system for systematically analyzing the influence of geometry on the self-assembly of 200 and 500 µm cubes and octahedra from tethered, multi-component, two-dimensional (2D) nets. We examined the self-assembly of all eleven 2D nets that can fold into cubes and octahedra, and we observed striking correlations between the compactness of the nets and the success of the assembly. Two measures of compactness were used for the nets: the number of vertex or topological connections and the radius of gyration. The success of the self-assembly process was determined by measuring the yield and classifying the defects. Our observation of increased self-assembly success with decreased radius of gyration and increased topological connectivity resembles theoretical models that describe the role of compactness in protein folding. Because of the differences in size and scale between our system and the protein folding system, we postulate that this hypothesis may be more universal to self-assembling systems in general. Apart from being intellectually intriguing, the findings could enable the assembly of more complicated polyhedral structures (e.g. dodecahedra) by allowing a priori selection of a net that might self-assemble with high yields

Effect of SiO2 nanoparticles on drought resistance in hawthorn seedlings

Drought is a significant factor limiting crop production in arid regions while hawthorns (Crataegus sp.) are an important component of such region’s forests. Therefore, treatments that increase hawthorn drought resistance may also increase transplanting success. Thus, the physiological and biochemical responses of hawthorn seedlings to a factorial combination of different concentrations of silica nanoparticles (SNPs at 0, 10, 50 and 100 mg L-1) and three soil moisture treatments (without stress, moderate stress and severe stress) were investigated. Seedlings were irrigated with one of the four concentrations of SNPs for 45 days before exposing them to drought stress. Photosynthesis parameters, malondialdehyde (MDA), relative water content (RWC ), membrane electrolyte leakage (ELI) as well as chlorophyll, carotenoid, carbohydrate and proline content were determined. At the end of the experiment, positive effects by SNP pre-treatment on physiological indexes were observed during drought stress. Under drought conditions, the effect of SNPs on photosynthetic rate and stomatal conductance was evident. Although the SNPs increased plant biomass, xylem water potential and MDA content, especially under drought conditions, RWC and ELI were not affected by the SNP pre-treatments. Seedlings pre-treated with SNPs had a decreased carbohydrate and proline content under all water regimes, but especially so under drought. Total chlorophyll content and carotenoid content did not change among the treatments. Generally, the findings imply that SNPs play a positive role in maintaining critical physiological and biochemical functions in hawthorn seedlings under drought stress conditions. However, more studies are needed before the physiological and biochemical basis of induced drought resistance can be determined

Three‐Dimensional Fabrication at Small Size Scales

Despite the fact that we live in a three-dimensional (3D) world and macroscale engineering is 3D, conventional sub-mm scale engineering is inherently two-dimensional (2D). New fabrication and patterning strategies are needed to enable truly three-dimensionally-engineered structures at small size scales. Here, we review strategies that have been developed over the last two decades that seek to enable such millimeter to nanoscale 3D fabrication and patterning. A focus of this review is the strategy of self-assembly, specifically in a biologically inspired, more deterministic form known as self-folding. Self-folding methods can leverage the strengths of lithography to enable the construction of precisely patterned 3D structures and “smart” components. This self-assembling approach is compared with other 3D fabrication paradigms, and its advantages and disadvantages are discussed

Effects of elevational gradient on leaf and stomatal morphology of Caucasian alder (Alnus subcordata) in the Hyrcanian forest, Iran

Land use/land cover change (LULC) is a major threat to natural protected areas worldwide. This paper explores the relationships between four estimated LULC parameters for 17 Mexican biosphere reserves (BRs) for 1993–2002 on a GIS platform, and ten socioeconomic factors obtained from census data. These relationships were tested through linear correlations and multivariate analysis. BRs showed lower human demographic pressure, but higher population dispersion, social marginality, percentage of rain-fed agriculture area, and dependence upon agriculture and cattle compared to nationwide values. BRs also varied in their indigenous population, and showed cattle overpopulation, and low immigration and road density. Socioeconomic factors explained 87% of LULC variation. High population and road density, cattle overpopulation and low percentage indigenous population were related to percentage of transformed area (2002). Conversely, small population and road density, large proportion of indigenous population and high dependency on agriculture and cattle, were related to the rate of change in transformed area (1993–2002). High human population growth and urban concentration occurred when BRs suffered higher LULC than their corresponding ecoregions. Including socioeconomic conditions prevailing in BRs and their influence on LULC in reserve management and rural development planning will improve strategies for the confluence of conservation and development goals

Self-loading lithographically structured microcontainers: 3D patterned, mobile microwells

We demonstrate mass-producible, mobile, self-loading microcontainers that can be used to encapsulate both non-living and living objects, thus forming three-dimensionally patterned, mobile microwells