Note, The Convention for the Elimination of All Forms of Discrimination Against Women: Radical, Reasonable, or Reactionary?

This Note will explore the merits behind these positions and attempt a resolution. If the potential effect of the Convention can only be to freeze and enshrine sex equality law as it currently exists, one who is interested in achieving changes in the law for the purpose of benefiting women will not want to put her energy into lobbying for ratification. It is therefore important to get past political strategies and determine what promise the Convention might hold for women in the United States. If the United States were to ratify the Convention, what changes, if any, would result

Admissions of a Director

The admissions process at one of the nation\u27s most prestigious law schools is as much art as it is science. Sarah Zearfoss, Director of Admissions and Assistant Dean at the University of Michigan Law School, offers a firsthand account of the school\u27s admissions program. In the process she answers the commonly asked question, just what do admissions committees take into account when they review an application? She describes the Law School\u27s admissions program from a functional point of view, and explains why the concept of diversity embodies far more than race. She also refutes Justice Rehnquist\u27s analysis in dissent in Grutter v. Bollinger by explaining how the number of accepted students from each group is a function of the number of applicants, rather than an intentional numerical quota

Admissions of a Director

The admissions process at one of the nation\u27s most prestigious law schools is as much art as it is science. Sarah Zearfoss, Director of Admissions and Assistant Dean at the University of Michigan Law School, offers a firsthand account of the school\u27s admissions program. In the process she answers the commonly asked question, just what do admissions committees take into account when they review an application? She describes the Law School\u27s admissions program from a functional point of view, and explains why the concept of diversity embodies far more than race. She also refutes Justice Rehnquist\u27s analysis in dissent in Grutter v. Bollinger by explaining how the number of accepted students from each group is a function of the number of applicants, rather than an intentional numerical quota

Interacting partners of the SUNN symbiotic kinase

The control of nodule number in legumes is primarily accomplished through a complex systemic signaling pathway termed autoregulation of nodulation (AON). The protein kinase SUNN, a leucine-rich repeat receptor-like kinase, exhibits shoot control of AON. I show subcellular localization of SUNN to the plasma membrane and specifically to the plasmodesmata, a prime location for systemic signaling. To aid in better understanding the mechanisms of signaling in AON, I used different approaches to identify potential protein-protein interactions involving SUNN, including candidate genes, exploratory proteomics, and forward genetic screens. Candidate interactors, including CLAVATA2 (CLV2), CORYNE (CRN) and guanine exchange factors of the RopGEF family, were investigated using the bimolecular fluorescence complementation (BiFC) assay with SUNN and mutant analysis for their role in AON. I report positive BiFC interactions between SUNN and CLV2, CRN, RopGEF1, RopGEF2, and RopGEF5. Mutant analysis of these candidate proteins provide additional support for a role in AON. In legumes, mutations in either CLV2 or CRN, and RNAi of RopGEF1, RopGEF2, or RopGEF5 in roots render hypernodulation phenotypes. Taken together, my results suggest a signaling complex involving SUNN is formed in the shoots to transduce a signal from the roots into a signal back to the roots through the activation of GTPases by RopGEFs, ultimately regulating nodulation events. I also identified 22 novel putative interactors of SUNN stemming from an exploratory proteomics experiment involving transgenic M. truncatula carrying SUNN-YFP/HA that were subjected to co-immunoprecipitation to isolate protein complexes that contain the transgene. The precipitated proteins were identified by LC-MS/MS and are presented here as putative interacting partners. In addition, a forward genetics approach identified components of the AON signaling pathway. Utilizing a genetic suppressor screen of sunn-1, we identified four independent lines carrying mutations that suppress the supernodulation phenotype of sunn-1. The mapping of one of these suppressor lines pinpointed the location of the lesion to an approximately 150 kB region on Linkage Group 2 harboring about 26 annotated genes

Artificially Alternate Bauhaus: Gremlins of Function, Body, and Pattern

Human and machine intelligence has the potential to re-design objects and spaces of the past and generate an artificially alternate collection of reimagined Bauhaus images. Present A.I. technologies can operate as synthetic imaginations of historic Bauhaus principles and design ambitions to create a possible future. The past becomes altered through our understanding of its contributions to an alternate present

Localisation of RNAs into the germ plasm of vitellogenic xenopus oocytes

We have studied the localisation of mRNAs in full-grown Xenopus laevis oocytes by injecting fluorescent RNAs, followed by confocal microscopy of the oocyte cortex. Concentrating on RNA encoding the Xenopus Nanos homologue, nanos1 (formerly Xcat2), we find that it consistently localised into aggregated germ plasm ribonucleoprotein (RNP) particles, independently of cytoskeletal integrity. This implies that a diffusion/entrapment-mediated mechanism is active, as previously reported for previtellogenic oocytes. Sometimes this was accompanied by localisation into scattered particles of the “late”, Vg1/VegT pathway; occasionally only late pathway localisation was seen. The Xpat RNA behaved in an identical fashion and for neither RNA was the localisation changed by any culture conditions tested. The identity of the labelled RNP aggregates as definitive germ plasm was confirmed by their inclusion of abundant mitochondria and co-localisation with the germ plasm protein Hermes. Further, the nanos1/Hermes RNP particles are interspersed with those containing the germ plasm protein Xpat. These aggregates may be followed into the germ plasm of unfertilized eggs, but with a notable reduction in its quantity, both in terms of injected molecules and endogenous structures. Our results conflict with previous reports that there is no RNA localisation in large oocytes, and that during mid-oogenesis even germ plasm RNAs localise exclusively by the late pathway. We find that in mid oogenesis nanos1 RNA also localises to germ plasm but also by the late pathway. Late pathway RNAs, Vg1 and VegT, also may localise into germ plasm. Our results support the view that mechanistically the two modes of localisation are extremely similar, and that in an injection experiment RNAs might utilise either pathway, the distinction in fates being very subtle and subject to variation. We discuss these results in relation to their biological significance and the results of others

hnRNP A1 and secondary structure coordinate alternative splicing of Mag

Myelin-associated glycoprotein (MAG) is a major component of myelin in the vertebrate central nervous system. MAG is present in the periaxonal region of the myelin structure, where it interacts with neuronal proteins to inhibit axon outgrowth and protect neurons from degeneration. Two alternatively spliced isoforms of Mag mRNA have been identified. The mRNA encoding the shorter isoform, known as S-MAG, contains a termination codon in exon 12, while the mRNA encoding the longer isoform, known as L-MAG, skips exon 12 and produces a protein with a longer C-terminal region. L-MAG is required in the central nervous system. How inclusion of Mag exon 12 is regulated is not clear. In a previous study, we showed that heteronuclear ribonucleoprotein A1 (hnRNP A1) contributes to Mag exon 12 skipping. Here, we show that hnRNP A1 interacts with an element that overlaps the 5\u27 splice site of Mag exon 12. The element has a reduced ability to interact with the U1 snRNP compared with a mutant that improves the splice site consensus. An evolutionarily conserved secondary structure is present surrounding the element. The structure modulates interaction with both hnRNP A1 and U1. Analysis of splice isoforms produced from a series of reporter constructs demonstrates that the hnRNP A1-binding site and the secondary structure both contribute to exclusion of Mag exon 12

A molecular circuit composed of CPEB-1 and c-Jun controls growth hormone-mediated synaptic plasticity in the mouse hippocampus

Cytoplasmic polyadenylation element binding protein 1 (CPEB-1) resides at postsynaptic sites in hippocampal neurons in which it controls polyadenylation-induced translation. CPEB-1 knock-out (KO) mice display defects in some forms of synaptic plasticity and hippocampal-dependent memories. To identify CPEB-1-regulated mRNAs, we used proteomics to compare polypeptides in wild-type (WT) and CPEB-1 KO hippocampus. Growth hormone (GH) was reduced in the KO hippocampus, as were the GH signaling molecules phospho-JAK2 and phospho-STAT3. GH mRNA and pre-mRNA were reduced in the KO hippocampus, suggesting that CPEB-1 controls GH transcription. The transcription factor c-Jun, which binds the GH promoter, was also reduced in the KO hippocampus, as was its ability to coimmunoprecipitate chromatin containing the GH promoter. CPEB-1 binds c-Jun 3\u27 untranslated region CPEs in vitro and coimmunoprecipitates c-Jun RNA in vivo. GH induces long-term potentiation (LTP) when applied to hippocampal slices from WT and CPEB-1 KO mice, but the magnitude of LTP induced by GH in KO mice is reduced. Pretreatment with GH did not reverse the LTP deficit observed in KO mice after theta-burst stimulation (TBS). Cordycepin, an inhibitor of polyadenylation, disrupted LTP induced by either GH application or TBS. Finally, GH application to hippocampal slices induced JAK2 phosphorylation in WT but not KO animals. These results indicate that CPEB-1 control of c-Jun mRNA translation regulates GH gene expression and resulting downstream signaling events (e.g., synaptic plasticity) in the mouse hippocampus

Assistive Intelligence: Replication and Mediation of Modern

This project is an exploration of frames of reference that guide human authorship and how they influence machine output . “Reference frames are [used] to make predictions, create plans, and perform movements.” (Hawkins 131). We guided our exploration under the principle that if we control who authored the frames of reference that the AI receives, then that would influence the output of the machine. However, we began to question to what degree we need to control the machine in order to have a valuable output –is human-intelligent authorship necessary for machine intelligence to function productively, or is machine intelligence enough of an author on its own? Through this exploration we began to understand machine and human intelligences as interdependent

A Molecular Circuit Composed of CPEB-1 and c-Jun Controls Growth Hormone-Mediated Synaptic Plasticity in the Mouse Hippocampus

Cytoplasmic polyadenylation element binding protein 1 (CPEB-1) resides at postsynaptic sites in hippocampal neurons in which it controls polyadenylation-induced translation. CPEB-1 knock-out (KO) mice display defects in some forms of synaptic plasticity and hippocampal-dependent memories. To identify CPEB-1-regulated mRNAs, we used proteomics to compare polypeptides in wild-type (WT) and CPEB-1 KO hippocampus. Growth hormone (GH) was reduced in the KO hippocampus, as were the GH signaling molecules phospho-JAK2 and phospho-STAT3. GH mRNA and pre-mRNA were reduced in the KO hippocampus, suggesting that CPEB-1 controls GH transcription. The transcription factor c-Jun, which binds the GH promoter, was also reduced in the KO hippocampus, as was its ability to coimmunoprecipitate chromatin containing the GH promoter. CPEB-1 binds c-Jun 3' untranslated region CPEs in vitro and coimmunoprecipitates c-Jun RNA in vivo. GH induces long-term potentiation (LTP) when applied to hippocampal slices from WT and CPEB-1 KO mice, but the magnitude of LTP induced by GH in KO mice is reduced. Pretreatment with GH did not reverse the LTP deficit observed in KO mice after theta-burst stimulation (TBS). Cordycepin, an inhibitor of polyadenylation, disrupted LTP induced by either GH application or TBS. Finally, GH application to hippocampal slices induced JAK2 phosphorylation in WT but not KO animals. These results indicate that CPEB-1 control of c-Jun mRNA translation regulates GH gene expression and resulting downstream signaling events (e.g., synaptic plasticity) in the mouse hippocampus.VoRSUNY DownstateN/