Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation.

Bronchial epithelial cells play a pivotal role in airway inflammation, but little is known about posttranscriptional regulation of mediator gene expression during the inflammatory response in these cells. Here, we show that activation of human bronchial epithelial BEAS-2B cells by proinflammatory cytokines interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-alpha) leads to an increase in the mRNA stability of the key chemokines monocyte chemotactic protein 1 and IL-8, an elevation of the global translation rate, an increase in the levels of several proteins critical for translation, and a reduction of microRNA-mediated translational repression. Moreover, using the BEAS-2B cell system and a mouse model, we found that RNA processing bodies (P bodies), cytoplasmic domains linked to storage and/or degradation of translationally silenced mRNAs, are significantly reduced in activated bronchial epithelial cells, suggesting a physiological role for P bodies in airway inflammation. Our study reveals an orchestrated change among posttranscriptional mechanisms, which help sustain high levels of inflammatory mediator production in bronchial epithelium during the pathogenesis of inflammatory airway diseases

Die analyse eines mathematischen Modells und der Regressionsgleichung für die berechnung inerziäller Parameter von Körperteilen in jungen Chinesinnen

Objective: In this study a mathematical model was set up for calculating the inertial parameters of body segments in young Chinese female students. Methods: On the sample of 50 young Chinese women the inertial parameters, mass and mass center of body segments, were determined by using the Computed Tomography – Digital Image Processing (CT-DIP) method. Results: A 16-segment mathematical model of young Chinese women was set up and a binary regression equation for inertial parameters of body segments calculation was established, in which body weight and stature were treated as independent variables. Conclusion: The study provided a method for a simple calculation of mass, mass centre and moment of inertia both of the segments and of the total body in the population of young Chinese womenUvod Od samih početaka proučavanja čovječjeg kretanja, koncept fizikalnog modeliranja tijela bio je imanentan tim istraživanjima. Braune i Fischer, Hanavan, Hatze, Delp – autori su koji su obilježili razvoj modeliranja ljudskog tijela s primjenama u kineziologiji. Ovaj rad temelji se na pristupu Millerove i Nelsona, odnosno Zatsiorskyog i Seluyanova. Cilj je bio odrediti i analizirati inercijske podatke tijela mladih kineskih žena. Metode Uzorak je obuhvatio 50 žena, studentica, u dobi od 18 do 23 godine. Uporabljena je metoda Computed Tomography – Digital Image Processing (CT-DIP) za analizu 16 segmenata tijela. Na temelju razine sive boje i gustoće svake strukture i tkiva u slikama tih segmenata, inercijski parametri segmenata izracunati su metodom konačnih jedinica. Na kraju su procijenjeni masa, središte mase i moment inercije segmenta. Matematički model je opisan kao n-segmentni sustav s više stupnjeva slobode na spojevima segmenata. To je sustav sastavljen od niza pretpostavljeno homogenih krutih tijela jednostavnih geometrijskih oblika koji su spojeni u zglobovima, a na temelju Hanavanova koncepta 16-segmentnog sustava. Utvrđena je binarna regresijska jednadžba kojom se može izračunati masa, središte mase i moment inercije tjelesnih segmenata. Tjelesna masa i visina korištene su kao nezavisne varijable. Rezultati Prikazani su prosječni podaci za masu, središte mase i moment inercije segmenta i cijelog tijela izračunati metodom CT-DIP. Prikazani su kompletni rezultati dizajna 16- segmentnog modela tijela i inercijalni parametri izračunati binarnom regresijskom jednadžbom. Rasprava Uporabom balansne ploče i metode CT-DIP određeni su i uspoređeni inercijski podaci tijela. Pored standardnih nalaza, autori konstatiraju da ovakav pristup odražava različite karakteristike različitih ljudskih rasa. U okviru evaluacije binarne regresijske jednadžbe najveće diskrepancije u rezultatima pojavile su se u rezultatima za šake i stopala. Međutim, te diskrepancije nisu ozbiljnije utjecale na izračune jer su njihovi momenti bili izuzetno maleni u usporedbi s onima ostalih segmenata. U usporedbi izračunatih vrijednosti i onih dobivenih na balansnoj platformi najveća apsolutna varijanca mase cijeloga tijela iznosila je 3.01 kg, najveća relativna varijacija 3.88%, najveća apsolutna varijanca središta mase cijelog tijela 3.78 cm i najveća relativna varijacija 3.75%. Zaključak Provedena studija pokazala je da je pristup matematičkim modeliranjem dobro prilagođen populaciji mladih kineskih žena. Binarna regresijska jednadžba pokazala se jednostavnim, a vrijednim sredstvom u primjeni tog postupka. Potrebna su daljnja istraživanja da bi se povećala pouzdanost zaključaka.Ziel: In dieser Studie wurde ein mathematisches Modell für die Berechunng inerziäller Parameter von Hörperteilen in jungen Chinesinnen gestaltet. Methoden: Inerziälle Parameter, die Masse und das Massenzentrum von Hörperteilen wurden bei 50 jungen Chinesinnen mittels der Digitalen Bildbearbeitungsmethode (Digital Image Processing – DIP) bestimmt. Ergebnisse: 16-teiliges mathematisches Modell von jungen Chinesinnen wurde gestaltet zusammen mit der binären Regressionsgleichung für die Berechnung von inerziällen Parametern des Hörpergewichts und der Hörperteile. Hörpergewicht und Statur wurden als unabhängige Variablen betrachtet. Schlussfolgerung: Das Resultat dieser Analyse war die Gestaltung einer Methode für die einfache Berechnung von Masse, Massenzentrum und Inerzmoment einzelner Hörperteile und des ganzen Hörpers bei jungen Chinesinnen

Blockchain-based and verifiable multidimensional data aggregation and sharing scheme for smart grid

Aiming at the problem that how to support lightweight multi-dimensional data aggregation, achieve double-end integrity verification of multi-dimensional data in the overall process of the system, and deal with the centralization of cloud servers, a blockchain-based and verifiable multidimensional data aggregation and sharing scheme for smart grid was proposed.Firstly, in order to meet the demand for fine-grained analysis of power data in smart grid, privacy-preserving multidimensional data aggregation was achieved by using masked values and Horner’s rule.On this basis, for the data integrity verification problem of the existing data aggregation scheme in the sharing of cloud storage data with the third party, a new signature algorithm was designed by borrowing the RSA-based multiplicative homomorphic commitment scheme and homomorphic hash function homomorphism, which enabled the cloud server to verify the integrity of aggregated data and be used for integrity verification in the data sharing phase, i.e., it achieved double-end verifiability of cloud storage data and was resistant to internal attacks.Meanwhile, an aggregated data sharing architecture based on federated chain multichain was proposed to effectively avoid centralisation problems such as single-machine processing bottleneck and vulnerability to attacks.The theoretical analysis proves the security of the scheme.Performance experiments show that the proposed scheme has lower computation and communication costs compared with existing schemes

Exploration of the Minimum Necessary FVIII Level at Different Physical Activity Levels in Pediatric Patients with Hemophilia A

BACKGROUND: Physical activity can increase joint stability and reduce the risk of injury in hemophilia patients. There is limited clinical data on target trough FVIII levels during physical activity in hemophilia A patients. Hence, this study aimed to explore the target trough FVIII level required to avoid bleeding during different physical activities in hemophilia A patients. METHODS: Patients with severe or moderate hemophilia A, who underwent pharmacokinetics (PK) tests at our center were enrolled in this study. Physical activities and clinical information such as bleeding were recorded. The FVIII level during physical activity was calculated by the WAPPS-Hemo. RESULTS: A total of 105 patients were enrolled in this study. A total of 373 physical activities were recorded, of which 57.6% (215/373) was low-risk activities and the remaining 42.4% (158/373) was medium-risk activities. Most common physical activities were bicycling (59.0%), swimming (43.8%), running (48.6%), and jumping rope (41.0%). The FVIII trough level of low-risk physical activity was 3.8 IU/dl (AUC = 0.781, CONCLUSION: The minimum necessary FVIII level increased with higher risk physical activity, irrespective of arthropathy

Construction Technology and Application of TBM in Shengli Tunnel of Tianshan Mountain under poor geological conditions

            When a tunnel boring machine (TBM) excavates in poor geological conditions like fracture zones, soft rock with high ground stress, hard rock with high ground stress, and water inrush ground, severe problems such as surrounding rock collapse, convergence, rock burst and water and mud outburst are usually encountered. These problems existed in the Tianshan Shengli Tunnel project in Xinjiang, China. Several methods and techniques were adopted to reduce construction risks and improve construction efficiency. The seismic wave reflection long-distance geological forecasting method was employed to forecast and predict these adverse geological conditions. The pre-reinforcement construction method was employed on the surrounding broken rocks in front of the tunnel face to lower the risks of tunnel collapse and machine jamming. A combined steel arch was applied to prevent tunnel arch collapse. An artificial cap method was applied to prevent the jamming caused by high-ground stress soft rock. Measures such as spraying water on the tunnel face and drilling stress holes were employed to prevent rock bursts caused by high-ground stress in hard rock. Implementing the advanced pipe shed method during water influx, sudden mud, and other working conditions reduced the risk of groundwater damage to equipment and lowered risks to personnel safety. The successful application of these techniques can provide a reference for TBM tunnel construction in similar ground conditions

Low-Dose Immune Tolerance Induction in Children With Severe Hemophilia A With High-Titer Inhibitors: Type of Factor 8 Mutation and Outcomes

BACKGROUND: No studies evaluated the role of OBJECTIVES: To explore the association between METHODS: Children SHA with high-titer inhibitors who received low-dose ITI therapy at least for 1 year were included in this study. Based on the risk of inhibitor development, RESULTS: Of 104 children included, 101 had CONCLUSIONS: Types o

Flexible Coherent Optical Access: Architectures, Algorithms, and Demonstrations

To cope with the explosive bandwidth demand, significant progress has been made in the ITU-T standardization sector to define a higher-speed passive optical network (PON) with a 50Gb/s line rate. Recently, 50G PON becomes mature gradually, which means it is time to discuss beyond 50G PON. For ensuring an acceptable optical power budget, beyond 50G PON will potentially use coherent technologies, which can simultaneously promote the applications of flexible multiple access such as time/frequency-domain multiple access (TFDMA). In this paper, we will introduce the architectures, algorithms, and demonstrations for TFDMA-based coherent PON. The system architectures based on an ultra-simple coherent transceiver and specific signal spectra are designed to greatly reduce the cost of ONUs. Meanwhile, fast and low-complexity digital signal processing (DSP) algorithms are proposed for dealing with upstream and downstream signals. Based on the architectures and algorithms, we experimentally demonstrate the first real-time TFDMA-based coherent PON, which can support at most 256 end users, and peak line rates of 100Gb/s and 200Gb/s in the upstream and downstream scenarios, respectively. In conclusion, the proposed technologies for the coherent PON make it more possible to be applied in the future beyond 50G PON.Comment: The paper has been submitted to the Journal of Lightwave Technolog