Reversal or no reversal: the evolution of the star formation rate–density relation up to z ∼ 1.6

We investigate the evolution of the star formation rate (SFR)–density relation in the Extended Chandra Deep Field South and the Great Observatories Origin Deep Survey fields up to z ∼ 1.6. In addition to the ‘traditional method’, in which the environment is defined according to a statistical measurement of the local galaxy density, we use a ‘dynamical’ approach, where galaxies are classified according to three different environment regimes: group, ‘filament-like’ and field. Both methods show no evidence of an SFR–density reversal. Moreover, group galaxies show a mean SFR lower than other environments up to z ∼ 1, while at earlier epochs group and field galaxies exhibit consistent levels of star formation (SF) activity. We find that processes related to a massive dark matter halo must be dominant in the suppression of the SF below z ∼ 1, with respect to purely density-related processes. We confirm this finding by studying the distribution of galaxies in different environments with respect to the so-called main sequence (MS) of star-forming galaxies. Galaxies in both group and ‘filament-like’ environments preferentially lie below the MS up to z ∼ 1, with group galaxies exhibiting lower levels of star-forming activity at a given mass. At z > 1, the star-forming galaxies in groups reside on the MS. Groups exhibit the highest fraction of quiescent galaxies up to z ∼ 1, after which group, ‘filament-like’ and field environments have a similar mix of galaxy types. We conclude that groups are the most efficient locus for SF quenching. Thus, a fundamental difference exists between bound and unbound objects, or between dark matter haloes of different masses

Lipid storage and autophagy in melanoma cancer cells

Cancer stem cells (CSC) represent a key cellular subpopulation controlling biological features such as cancer progression in all cancer types. By using melanospheres established from human melanoma patients, we compared less differentiated melanosphere-derived CSC to differentiating melanosphere-derived cells. Increased lipid uptake was found in melanosphere-derived CSC vs. differentiating melanosphere-derived cells, paralleled by strong expression of lipogenic factors Sterol Regulatory Element-Binding Protein-1 (SREBP-1) and Peroxisome Proliferator-Activated Receptor-γ (PPAR-γ). An inverse relation between lipid-storing phenotype and autophagy was also found, since microtubule-associated protein 1A/1B-Light Chain 3 (LC3) lipidation is reduced in melanosphere-derived CSC. To investigate upstream autophagy regulators, Phospho-AMP activated Protein Kinase (P-AMPK) and Phospho-mammalian Target of Rapamycin (P-mTOR) were analyzed; lower P-AMPK and higher P-mTOR expression in melanosphere-derived CSC were found, thus explaining, at least in part, their lower autophagic activity. In addition, co-localization of LC3-stained autophagosome spots and perilipin-stained lipid droplets was demonstrated mainly in differentiating melanosphere-derived cells, further supporting the role of autophagy in lipid droplets clearance. The present manuscript demonstrates an inverse relationship between lipid-storing phenotype and melanoma stem cells differentiation, providing novel indications involving autophagy in melanoma stem cells biology

The XXL Survey X: K-band luminosity - weak-lensing mass relation for groups and clusters of galaxies

We present the K-band luminosity-halo mass relation, $L_{K,500}-M_{500,WL}$, for a subsample of 20 of the 100 brightest clusters in the XXL Survey observed with WIRCam at the Canada-France-Hawaii Telescope (CFHT). For the first time, we have measured this relation via weak-lensing analysis down to $M_{500,WL} =3.5 \times 10^{13}\,M_\odot$. This allows us to investigate whether the slope of the $L_K-M$ relation is different for groups and clusters, as seen in other works. The clusters in our sample span a wide range in mass, $M_{500,WL} =0.35-12.10 \times 10^{14}\,M_\odot$, at $0<z<0.6$. The K-band luminosity scales as $\log_{10}(L_{K,500}/10^{12}L_\odot) \propto \beta log_{10}(M_{500,WL}/10^{14}M_\odot)$ with $\beta = 0.85^{+0.35}_{-0.27}$ and an intrinsic scatter of $\sigma_{lnL_K|M} =0.37^{+0.19}_{-0.17}$. Combining our sample with some clusters in the Local Cluster Substructure Survey (LoCuSS) present in the literature, we obtain a slope of $1.05^{+0.16}_{-0.14}$ and an intrinsic scatter of $0.14^{+0.09}_{-0.07}$. The flattening in the $L_K-M$ seen in previous works is not seen here and might be a result of a bias in the mass measurement due to assumptions on the dynamical state of the systems. We also study the richness-mass relation and find that group-sized halos have more galaxies per unit halo mass than massive clusters. However, the brightest cluster galaxy (BCG) in low-mass systems contributes a greater fraction to the total cluster light than BCGs do in massive clusters; the luminosity gap between the two brightest galaxies is more prominent for group-sized halos. This result is a natural outcome of the hierarchical growth of structures, where massive galaxies form and gain mass within low-mass groups and are ultimately accreted into more massive clusters to become either part of the BCG or one of the brighter galaxies. [Abridged]Comment: A&A, in pres

TLR3 engagement induces IRF-3-dependent apoptosis in androgen-sensitive prostate cancer cells and inhibits tumour growth in vivo

Toll-like receptors (TLRs) are a family of highly conserved transmembrane proteins expressed in epithelial and immune cells that recognize pathogen associated molecular patterns. Besides their role in immune response against infections, numerous studies have shown an important role of different TLRs in cancer, indicating these receptors as potential targets for cancer therapy. We previously demonstrated that the activation of TLR3 by the synthetic double-stranded RNA analogue poly I:C induces apoptosis of androgen-sensitive prostate cancer (PCa) LNCaP cells and, much less efficiently, of the more aggressive PC3 cell line. Therefore, in this study we selected LNCaP cells to investigate the mechanism of TLR3-mediated apoptosis and the in vivo efficacy of poly I:C-based therapy. We show that interferon regulatory factor-3 (IRF-3) signalling plays an essential role in TLR3-mediated apoptosis in LNCaP cells through the activation of the intrinsic and extrinsic apoptotic pathways. Interestingly, hardly any apoptosis was induced by poly I:C in normal prostate epithelial cells RWPE-1. We also demonstrate for the first time the direct anticancer effect of poly I:C as a single therapeutic agent in a well-established human androgen-sensitive PCa xenograft model, by showing that tumour growth is highly impaired in poly I:C-treated immunodeficient mice. Immunohistochemical analysis of PCa xenografts highlights the antitumour role of poly I:C in vivo both on cancer cells and, indirectly, on endothelial cells. Notably, we show the presence of TLR3 and IRF-3 in both human normal and PCa clinical samples, potentially envisaging poly I:C-based therapy for PCa

Interference coloration as an anti-predator defence

Interference coloration, in which the perceived colour varies predictably with the angle of illumination or observation, is extremely widespread across animal groups. However, despite considerable advances in our understanding of the mechanistic basis of interference coloration in animals, we still have a poor understanding of its function. Here, I show, using avian predators hunting dynamic virtual prey, that the presence of interference coloration can significantly reduce a predator's attack success. Predators required more pecks to successfully catch interference-coloured prey compared with otherwise identical prey items that lacked interference coloration, and attacks against prey with interference colours were less accurate, suggesting that changes in colour or brightness caused by prey movement hindered a predator's ability to pinpoint their exact location. The pronounced antipredator benefits of interference coloration may explain why it has evolved independently so many times. © 2015 The Author(s) Published by the Royal Society. All rights reserved

The evolution of galaxy star formation activity in massive halos

There is now a large consensus that the current epoch of the Cosmic Star Formation History (CSFH) is dominated by low mass galaxies while the most active phase at 1<z<2 is dominated by more massive galaxies, which undergo a faster evolution. Massive galaxies tend to inhabit very massive halos such as galaxy groups and clusters. We aim to understand whether the observed "galaxy downsizing" could be interpreted as a "halo downsizing", whereas the most massive halos, and their galaxy populations, evolve more rapidly than the halos of lower mass. Thus, we study the contribution to the CSFH of galaxies inhabiting group-sized halos. This is done through the study of the evolution of the Infra-Red (IR) luminosity function of group galaxies from redshift 0 to ~1.6. We use a sample of 39 X-ray selected groups in the Extended Chandra Deep Field South (ECDFS), the Chandra Deep Field North (CDFN), and the COSMOS field, where the deepest available mid- and far-IR surveys have been conducted with Spitzer MIPS and Hersche PACS. Groups at low redshift lack the brightest, rarest, and most star forming IR-emitting galaxies observed in the field. Their IR-emitting galaxies contribute <10% of the comoving volume density of the whole IR galaxy population in the local Universe. At redshift >~1, the most IR-luminous galaxies (LIRGs and ULIRGs) are preferentially located in groups, and this is consistent with a reversal of the star-formation rate vs .density anti-correlation observed in the nearby Universe. At these redshifts, group galaxies contribute 60-80% of the CSFH, i.e. much more than at lower redshifts. Below z~1, the comoving number and SFR densities of IR-emitting galaxies in groups decline significantly faster than those of all IR-emitting galaxies. Our results are consistent with a "halo downsizing" scenario and highlight the significant role of "environment" quenching in shaping the CSFH.Comment: 14 pages, 10 figures, accepted for publication by A&

Stem-like and highly invasive prostate cancer cells expressing CD44v8-10 marker originate from CD44-negative cells

In human prostate cancer (PCa), the neuroendocrine cells, expressing the prostate cancer stem cell (CSC) marker CD44, may be resistant to androgen ablation and promote tumor recurrence. During the study of heterogeneity of the highly aggressive neuroendocrine PCa cell lines PC3 and DU-145, we isolated and expanded in vitro a minor subpopulation of very small cells lacking CD44 (CD44neg). Unexpectedly, these sorted CD44neg cells rapidly and spontaneously converted to a stable CD44high phenotype specifically expressing the CD44v8-10 isoform which the sorted CD44high subpopulation failed to express. Surprisingly and potentially interesting, in these cells expression of CD44v8-10 was found to be induced in stem cell medium. CD44 variant isoforms are known to be more expressed in CSC and metastatic cells than CD44 standard isoform. In agreement, functional analysis of the two sorted and cultured subpopulations has shown that the CD44v8-10pos PC3 cells, resulting from the conversion of the CD44neg subpopulation, were more invasive in vitro and had a higher clonogenic potential than the sorted CD44high cells, in that they produced mainly holoclones, known to be enriched in stem-like cells. Of interest, the CD44v8-10 is more expressed in human PCa biopsies than in normal gland. The discovery of CD44v8-10pos cells with stem-like and invasive features, derived from a minoritarian CD44neg cell population in PCa, alerts on the high plasticity of stem-like markers and urges for prudency on the approaches to targeting the putative CSC

Ultra-deep catalog of X-ray groups in the Extended Chandra Deep Field South

Ultra-deep observations of ECDF-S with Chandra and XMM-Newton enable a search for extended X-ray emission down to an unprecedented flux of $2\times10^{-16}$ ergs s$^{-1}$ cm$^{-2}$. We present the search for the extended emission on spatial scales of 32$^{\prime\prime}$ in both Chandra and XMM data, covering 0.3 square degrees and model the extended emission on scales of arcminutes. We present a catalog of 46 spectroscopically identified groups, reaching a redshift of 1.6. We show that the statistical properties of ECDF-S, such as logN-logS and X-ray luminosity function are broadly consistent with LCDM, with the exception that dn/dz/d$\Omega$ test reveals that a redshift range of $0.2<z<0.5$ in ECDF-S is sparsely populated. The lack of nearby structure, however, makes studies of high-redshift groups particularly easier both in X-rays and lensing, due to a lower level of clustered foreground. We present one and two point statistics of the galaxy groups as well as weak-lensing analysis to show that the detected low-luminosity systems are indeed low-mass systems. We verify the applicability of the scaling relations between the X-ray luminosity and the total mass of the group, derived for the COSMOS survey to lower masses and higher redshifts probed by ECDF-S by means of stacked weak lensing and clustering analysis, constraining any possible departures to be within 30% in mass. Abridged.Comment: 20 pages, 21 figures, 3 tables, to match the journal versio

The role of massive halos in the Star Formation History of the Universe

The most striking feature of the Cosmic Star Formation History (CSFH) of the Universe is a dramatic drop of the star formation (SF) activity, since z~1. In this work we investigate if the very same process of assembly and growth of structures is one of the major drivers of the observed decline. We study the contribution to the CSFH of galaxies in halos of different masses. This is done by studying the total SFR-halo mass-redshift plane from redshift 0 to redshift z~1.6 in a sample of 57 groups and clusters by using the deepest available mid- and far-infrared surveys conducted with Spitzer MIPS and Herschel PACS and SPIRE. Our results show that low mass groups provide a 60-80% contribution to the CSFH at z~1. Such contribution declines faster than the CSFH in the last 8 billion years to less than 10% at z<0.3, where the overall SF activity is sustained by lower mass halos. More massive systems provide only a marginal contribution (<10%) at any epoch. A simplified abundance matching method shows that the large contribution of low mass groups at z~1 is due to a large fraction (>50%) of very massive, highly star forming Main Sequence galaxies. Below z~1 a quenching process must take place in massive halos to cause the observed faster suppression of their SF activity. Such process must be a slow one though, as most of the models implementing a rapid quenching of the SF activity in accreting satellites significantly underpredicts the observed SF level in massive halos at any redshift. Starvation or the transition from cold to hot accretion would provide a quenching timescale of 1 Gyrs more consistent with the observations. Our results suggest a scenario in which, due to the structure formation process, more and more galaxies experience the group environment and, thus, the associated quenching process. This leads to the progressive suppression of their SF activity shaping the CSFH below z~1.Comment: 18 pages, 21 figures, accepted for publication by A&

Reversal or no reversal: the evolution of the star formation rate-density relation up to z~1.6

We investigate the evolution of the star formation rate (SFR)-density relation in the Extended Chandra Deep Field South (ECDFS) and the Great Observatories Origin Deep Survey (GOODS) fields up to z~1.6. In addition to the "traditional method", in which the environment is defined according to a statistical measurement of the local galaxy density, we use a "dynamical" approach, where galaxies are classified according to three different environment regimes: group, "filament-like", and field. Both methods show no evidence of a SFR-density reversal. Moreover, group galaxies show a mean SFR lower than other environments up to z~1, while at earlier epochs group and field galaxies exhibit consistent levels of star formation (SF) activity. We find that processes related to a massive dark matter halo must be dominant in the suppression of the SF below z~1, with respect to purely density-related processes. We confirm this finding by studying the distribution of galaxies in different environments with respect to the so-called Main Sequence (MS) of star-forming galaxies. Galaxies in both group and "filament-like" environments preferentially lie below the MS up to z~1, with group galaxies exhibiting lower levels of star-forming activity at a given mass. At z>1, the star-forming galaxies in groups reside on the MS. Groups exhibit the highest fraction of quiescent galaxies up to z~1, after which group, "filament-like", and field environments have a similar mix of galaxy types. We conclude that groups are the most efficient locus for star-formation quenching. Thus, a fundamental difference exists between bound and unbound objects, or between dark matter haloes of different masses.Comment: Accepted for publication in MNRA