Optical biopsy identification and grading of gliomas using label-free visible resonance Raman spectroscopy.

Glioma is one of the most refractory types of brain tumor. Accurate tumor boundary identification and complete resection of the tumor are essential for glioma removal during brain surgery. We present a method based on visible resonance Raman (VRR) spectroscopy to identify glioma margins and grades. A set of diagnostic spectral biomarkers features are presented based on tissue composition changes revealed by VRR. The Raman spectra include molecular vibrational fingerprints of carotenoids, tryptophan, amide I/II/III, proteins, and lipids. These basic in situ spectral biomarkers are used to identify the tissue from the interface between brain cancer and normal tissue and to evaluate glioma grades. The VRR spectra are also analyzed using principal component analysis for dimension reduction and feature detection and support vector machine for classification. The cross-validated sensitivity, specificity, and accuracy are found to be 100%, 96.3%, and 99.6% to distinguish glioma tissues from normal brain tissues, respectively. The area under the receiver operating characteristic curve for the classification is about 1.0. The accuracies to distinguish normal, low grade (grades I and II), and high grade (grades III and IV) gliomas are found to be 96.3%, 53.7%, and 84.1% for the three groups, respectively, along with a total accuracy of 75.1%. A set of criteria for differentiating normal human brain tissues from normal control tissues is proposed and used to identify brain cancer margins, yielding a diagnostic sensitivity of 100% and specificity of 71%. Our study demonstrates the potential of VRR as a label-free optical molecular histopathology method used for in situ boundary line judgment for brain surgery in the margins

Metasomatized lithospheric mantle for Mesozoic giant gold deposits in the North China craton

The origin of giant lode gold deposits of Mesozoic age in the North China craton (NCC) is enigmatic because high-grade metamorphic ancient crust would be highly depleted in gold. Instead, lithospheric mantle beneath the crust is the likely source of the gold, which may have been anomalously enriched by metasomatic processes. However, the role of gold enrichment and metasomatism in the lithospheric mantle remains unclear. Here, we present comprehensive data on gold and platinum group element contents of mantle xenoliths (n = 28) and basalts (n = 47) representing the temporal evolution of the eastern NCC. The results indicate that extensive mantle metasomatism and hydration introduced some gold (<1–2 ppb) but did not lead to a gold-enriched mantle. However, volatile-rich basalts formed mainly from the metasomatized lithospheric mantle display noticeably elevated gold contents as compared to those from the asthenosphere. Combined with the significant inheritance of mantle-derived volatiles in auriferous fluids of ore bodies, the new data reveal that the mechanism for the formation of the lode gold deposits was related to the volatile-rich components that accumulated during metasomatism and facilitated the release of gold during extensional craton destruction and mantle melting. Gold-bearing, hydrous magmas ascended rapidly along translithospheric fault zones and evolved auriferous fluids to form the giant deposits in the crust

Experiments on bright field and dark field high energy electron imaging with thick target material

Using a high energy electron beam for the imaging of high density matter with both high spatial-temporal and areal density resolution under extreme states of temperature and pressure is one of the critical challenges in high energy density physics . When a charged particle beam passes through an opaque target, the beam will be scattered with a distribution that depends on the thickness of the material. By collecting the scattered beam either near or off axis, so-called bright field or dark field images can be obtained. Here we report on an electron radiography experiment using 45 MeV electrons from an S-band photo-injector, where scattered electrons, after interacting with a sample, are collected and imaged by a quadrupole imaging system. We achieved a few micrometers (about 4 micrometers) spatial resolution and about 10 micrometers thickness resolution for a silicon target of 300-600 micron thickness. With addition of dark field images that are captured by selecting electrons with large scattering angle, we show that more useful information in determining external details such as outlines, boundaries and defects can be obtained.Comment: 7pages, 7 figure

TRUSS STRUCTURE OPTIMIZATION BASED ON IMPROVED WOLF PACK ALGORITHM

Aiming at the optimization of truss structure, a wolf pack algorithm based on chaos and improved search strategy was proposed. The mathematical model of truss optimization was constructed, and the classical truss structure was optimized. The results were compared with those of other optimization algorithms. When selecting and updating the initial position of wolves, chaos idea was used to distribute the initial value evenly in the solution space; phase factor was introduced to optimize the formula of wolf detection; information interaction between wolves is increased and the number of runs is reduced. The numerical results show that the improved wolf pack algorithm has the characteristics of fewer parameters, simple programming, easy implementation, fast convergence speed, and can quickly find the optimal solution. It is suitable for the optimization design of the section size of space truss structures

Divergence of the bZIP Gene Family in Strawberry, Peach, and Apple Suggests Multiple Modes of Gene Evolution after Duplication

The basic leucine zipper (bZIP) transcription factors are the most diverse members of dimerizing transcription factors. In the present study, 50, 116, and 47 bZIP genes were identified in Malus domestica (apple), Prunus persica (peach), and Fragaria vesca (strawberry), respectively. Species-specific duplication was the main contributor to the large number of bZIPs observed in apple. After WGD in apple genome, orthologous bZIP genes corresponding to strawberry on duplicated regions in apple genome were retained. However, in peach ancestor, these syntenic regions were quickly lost or deleted. Maybe the positive selection contributed to the expansion of clade S to adapt to the development and environment stresses. In addition, purifying selection was mainly responsible for bZIP sequence-specific DNA binding. The analysis of orthologous pairs between chromosomes indicates that these orthologs derived from one gene duplication located on one of the nine ancient chromosomes in the Rosaceae. The comparative analysis of bZIP genes in three species provides information on the evolutionary fate of bZIP genes in apple and peach after they diverged from strawberry

Towards Realistic Emotional Voice Conversion using Controllable Emotional Intensity

Realistic emotional voice conversion (EVC) aims to enhance emotional diversity of converted audios, making the synthesized voices more authentic and natural. To this end, we propose Emotional Intensity-aware Network (EINet), dynamically adjusting intonation and rhythm by incorporating controllable emotional intensity. To better capture nuances in emotional intensity, we go beyond mere distance measurements among acoustic features. Instead, an emotion evaluator is utilized to precisely quantify speaker's emotional state. By employing an intensity mapper, intensity pseudo-labels are obtained to bridge the gap between emotional speech intensity modeling and run-time conversion. To ensure high speech quality while retaining controllability, an emotion renderer is used for combining linguistic features smoothly with manipulated emotional features at frame level. Furthermore, we employ a duration predictor to facilitate adaptive prediction of rhythm changes condition on specifying intensity value. Experimental results show EINet's superior performance in naturalness and diversity of emotional expression compared to state-of-the-art EVC methods.Comment: Accepted to INTERSPEECH202

Learning Local to Global Feature Aggregation for Speech Emotion Recognition

Transformer has emerged in speech emotion recognition (SER) at present. However, its equal patch division not only damages frequency information but also ignores local emotion correlations across frames, which are key cues to represent emotion. To handle the issue, we propose a Local to Global Feature Aggregation learning (LGFA) for SER, which can aggregate longterm emotion correlations at different scales both inside frames and segments with entire frequency information to enhance the emotion discrimination of utterance-level speech features. For this purpose, we nest a Frame Transformer inside a Segment Transformer. Firstly, Frame Transformer is designed to excavate local emotion correlations between frames for frame embeddings. Then, the frame embeddings and their corresponding segment features are aggregated as different-level complements to be fed into Segment Transformer for learning utterance-level global emotion features. Experimental results show that the performance of LGFA is superior to the state-of-the-art methods.Comment: This paper has been accepted on INTERSPEECH 202